ABSTRACT The genes encoding tetrachloroethene reductive dehalogenase, a corrinoid-Fe/S protein, of Dehalospirillum multivorans were cloned and sequenced. pceA gene is upstream pceB overlaps it by 4 bp. presence ς70-like promoter sequence ρ-independent terminator downstream indicated that both are cotranscribed. This assumption supported reverse transcriptase PCR data. encode putative 501- 74-amino-acid proteins, respectively, with calculated molecular masses 55,887 8,354 Da, respectively....

Abstract The yeast strains Cryptococcus podzolicus , Trichosporon porosum and Pichia segobiensis were isolated from soil samples identified as oleaginous beneficial for the establishment of microbial production processes sustainable lipid suitable several industrial applications. When cultured in bioreactors with glucose sole carbon source C. yielded 31.8% per dry biomass at 20°C, while T. 34.1% 25°C P. 24.6% 25°C. These amounts correspond to concentrations 17.97 g/L, 17.02 g/L 12.7...

Synthesis gas (syngas) fermentation using acetogenic bacteria is an approach for production of bulk chemicals like acetate, ethanol, butanol or 2,3-butandiol avoiding the fuel vs. food debate by carbon monoxide, dioxide and hydrogen from gasification biomass industrial waste gases. Suffering energetic limitations, yields C4-molecules produced syngas are quite low compared with ABE sugars as a substrate. On other hand, fungal malic acid has high product per gram metabolized substrate but...

Low productivities of bioprocesses using gaseous carbon and energy sources are usually caused by the low solubility those gases (e.g., H2 CO). It has been suggested that increasing partial pressure will result in higher dissolved concentrations should, therefore, be helpful to overcome this obstacle. Investigations late 1980s with mixtures hydrogen monoxide showed inhibitory effects pressures above 0.8 bar. Avoiding any monoxide, we investigate growth product formation Clostridium...

The overreliance on dwindling fossil fuel reserves and the negative climatic effects of using such fuels are driving development new clean energy sources. One alternative source is hydrogen (H2), which can be generated from renewable Parageobacillus thermoglucosidasius a facultative anaerobic thermophilic bacterium frequently isolated high temperature environments including hot springs compost.Comparative genomics performed in present study showed that P. encodes two evolutionary distinct...

The integration of gasification with syngas fermentation is still in an early stage development, where many questions exist concerning the quality required. challenge to define conditions that will provide a H2:CO:CO2 ratio suitable for fermentation, as well identify and remove compounds can inhibit performance microorganisms. right balance between line up minimal gas cleaning needs be assessed improve economic feasibility process. In this work first attempt combine two processes presented,...

Abstract Feeding microbial communities with both organic and inorganic substrates can improve sustainability feasibility of chain elongation processes. Sustainably produced H 2 , CO be co‐fed to microorganisms as a source for acetyl‐CoA, while small amount an ATP‐generating substrate helps overcome the kinetic hindrances associated autotrophic carboxylate production. Here, we operated two semi‐continuous bioreactor systems continuous recirculation co‐feeding model feedstock (lactate acetate)...

Production of the Alternaria mycotoxins alternariol (AOH), monomethylether (AME) and tenuazonic acid (TA) by alternata DSM 12633 was influenced pH carbon to nitrogen (C:N) ratio growth medium both in shaking flasks bioreactor cultivation. The impact on mycotoxin production studied range 3.5 - 8. values above 5.5 led a decreased or inhibited formation completely whereas an acidic 4.0-4.5 optimal for production. influence C:N evaluated over 24 96. Glucose used as source its concentration...

A series of studies on the toxicity and nitrogen utilization biuret as a source NPN for sheep cattle is reported. Critical tests were conducted with sheep. Metabolism cattle. growth reproduction study was over period 593 days. The following general conclusions are drawn: Biuret fed at levels to supply significant part requirements neither acutely nor cumulatively toxic Growth, reproduction, wool production did not seem be adversely affected when biuret-containing rations. Positive balances...

The reductive dehalogenation of chlorinated propenes was studied with the tetrachloroethene dehalogenase purified from Sulfurospirillum multivorans to obtain indications for a radical mechanism this reaction. When reduced methyl viologen (MV), which is cation, applied as electron donor reduction different chloropropenes, significant part MV could not be rereduced Ti(III) citrate, indicating that consumed in side Mass spectrometric analysis assays revealed formation products, masses might...

Cyanide is a minor constituent of crude syngas whose content depends on the feedstock and gasification procedure. It known poison to metal catalysts inhibits iron-containing enzymes like carbon monoxide dehydrogenase acetogenic organisms. Therefore it considered component that has be removed from gas stream prior use in chemical synthesis or fermentation. We show growth rate maximum biomass concentration Clostridium ljungdahlii are unaffected by cyanide at concentrations up 1.0 mM with...

Feeder steers were depleted of liver vitamin A stores while wintered on corn silages containing carotenes in quantity considerably excess supposed needs. Steers maintained silage feeds through winter and spring developed deficiency even though supplemented throughout with 7,000 to 8,000 I.U. daily as preformed A. Such found be capable storing administered massive dose either orally or parenterally. feeds, failed improve during a subsequent period which mixed hay grain provided carotene...

Abstract Trichosporonaceae incorporates six genera of physiologically and ecologically diverse fungi including both human pathogenic taxa as well yeasts biotechnological interest, especially those oleagenic that accumulate large amounts single cell oils (SCOs). Here, we have undertaken comparative genomic analysis thirty-three members the family with a view to gain insight into molecular determinants underlying their lifestyles niche specializations. Phylogenomic revealed potential...

Syngas, the product of biomass gasification, can play an important role in moving towards production renewable chemical commodities, by using acetogenic bacteria to ferment those gaseous mixtures. Due complex and changing nature biomass, composition impurities present final biomass-derived syngas will vary. Because this, it is assess impact these factors on fermentation outcome, terms yields, productivity, formation ratio. In this study, Clostridium ljungdahlii was used a fed-batch system...