A5072921858- Muscle Physiology and Disorders
- RNA Research and Splicing
- RNA Interference and Gene Delivery
- Single-cell and spatial transcriptomics
- CRISPR and Genetic Engineering
- Molecular Biology Techniques and Applications
- Virus-based gene therapy research
- RNA and protein synthesis mechanisms
- CAR-T cell therapy research
- Neurogenetic and Muscular Disorders Research
- Viral Infections and Immunology Research
- Advanced biosensing and bioanalysis techniques
- Genomics and Chromatin Dynamics
- Reproductive Biology and Fertility
Broad Institute
2019-2023
Nationwide Children's Hospital
2020-2022
Single-cell RNA sequencing has revealed extensive molecular diversity in gene programs governing mammalian spermatogenesis but fails to delineate their dynamics the native context of seminiferous tubules, spatially confined functional units spermatogenesis. Here, we use Slide-seq, a spatial transcriptomics technology, generate an atlas that captures expression patterns at near-single-cell resolution mouse and human testis. Using Slide-seq data, devise computational framework accurately...
Abstract In biological systems, spatial organization is interconnected with genome function and regulation. However, methods that couple high-throughput genomic epigenomic profiling information are lacking. Here, we developed Photoselective Sequencing, a spatially-informed DNA sequencing method to assay collections of cells or subcellular regions share unifying morphological trait. prepare blocked fragment library within fixed specimen. Guided by fluorescence imaging, remove the block in...