A5054578101- Muscle Physiology and Disorders
- RNA Research and Splicing
- RNA Interference and Gene Delivery
- Virus-based gene therapy research
- Adipose Tissue and Metabolism
- Oral microbiology and periodontitis research
- RNA modifications and cancer
- Extracellular vesicles in disease
- Antimicrobial Peptides and Activities
- Adipokines, Inflammation, and Metabolic Diseases
- RNA regulation and disease
- CRISPR and Genetic Engineering
- Genetic Neurodegenerative Diseases
- Microtubule and mitosis dynamics
- Salivary Gland Disorders and Functions
- Diet and metabolism studies
- Neurogenetic and Muscular Disorders Research
- Viral Infections and Immunology Research
- Bacterial Infections and Vaccines
- Silk-based biomaterials and applications
- Cardiomyopathy and Myosin Studies
- Cellular Mechanics and Interactions
- Neonatal and Maternal Infections
Nationwide Children's Hospital
2021-2025
The Ohio State University
2023
The Ohio State University Wexner Medical Center
2021
Central Michigan University
2015
Therapeutic exon skipping as a treatment for Duchenne muscular dystrophy (DMD) has largely concentrated on the delivery of antisense oligomers to treat out-of-frame deletions. Here we report preclinical development an adeno-associated virus (AAV)-encapsidated viral vector containing four copies noncoding U7 small nuclear RNA (U7snRNA), each targeted either splice donor or acceptor sites DMD 2. We have previously shown that this (scAAV9.U7.ACCA) Dup2 mouse model results in expression...
Porphyromonas gingivalis (Pg) is an oral bacterial pathogen that has been associated with systemic inflammation and adverse pregnancy outcomes such as low birth weight pre-term birth. Pg drives these sequelae through virulence factors decorating the outer membrane are present on non-replicative vesicles (OMV) suspected to be transmitted systemically. Given abundance can increase during pregnancy, it not well known whether Pg-OMV have deleterious effects brain of developing fetus. We tested...
Abstract Aims Dystrophin, the protein product of DMD gene, plays a critical role in muscle integrity by stabilising sarcolemma during contraction and relaxation. The gene is vulnerable to variety mutations that may cause complete loss, depletion or truncation protein, leading Duchenne Becker muscular dystrophies. Precise reproducible dystrophin quantification essential characterising evaluating outcome efforts induce through therapies. Immunofluorescence microscopy offers high sensitivity...
Duchenne muscular dystrophy (DMD) is a devastating muscle-wasting disease that arises due to the loss of dystrophin expression, leading progressive motor and cardiorespiratory function. Four exon-skipping approaches using antisense phosphorodiamidate morpholino oligomers (PMOs) have been approved by FDA restore DMD open reading frame, resulting in expression functional but internally deleted protein, patients with single-exon duplications, exon skipping has potential full-length expression....
Duchenne muscular dystrophy (DMD) is a progressive X-linked disease caused by mutations in the DMD gene that prevent expression of functional dystrophin protein. Exon duplications represent 6%-11% mutations, and exon 2 (Dup2) are most common (∼11%) duplication mutations. An exon-skipping strategy for Dup2 presents large therapeutic window. Skipping one copy results full-length expression, whereas skipping both copies (Del2) activates an internal ribosomal entry site (IRES) 5, inducing highly...
Abstract Porphyromonas gingivalis (Pg) is an oral bacterial pathogen that has been associated with systemic inflammation and adverse pregnancy outcomes such as low birth weight preterm birth. Pg drives these sequelae through virulence factors decorating the outer membrane are present on non-replicative vesicles (OMV) suspected to be transmitted systemically. Given abundance can increase during pregnancy, it not well known whether Pg-OMV have deleterious effects brain of developing fetus. We...
A single bout of exercise (EX) can have benefits on glucose metabolism in both normal and insulin-resistant individuals. Several plasma proteins secreted by muscle other tissues been demonstrated to roles regulating energy including irisin, brain-derived neurotrophic factor (BDNF), fibroblast growth factor-21 (FGF-21). However the effect circulating levels these proteins, after an acute a model dietary-induced insulin resistance is not known. Rats (male Sprague-Dawley, ~9-mos old) were fed...