A5033142284- T-cell and B-cell Immunology
- Immunotherapy and Immune Responses
- Single-cell and spatial transcriptomics
- Immune Cell Function and Interaction
- Immune Response and Inflammation
- vaccines and immunoinformatics approaches
- RNA and protein synthesis mechanisms
- CRISPR and Genetic Engineering
- RNA regulation and disease
McGovern Institute for Brain Research
2024
Massachusetts Institute of Technology
2024
ETH Zurich
2022-2023
RNA editing offers the opportunity to introduce either stable or transient modifications nucleic acid sequence without permanent off-target effects, but installation of arbitrary edits into transcriptome is currently infeasible. Here, we describe Programmable Editing & Cleavage for Insertion, Substitution, and Erasure (PRECISE), a versatile method writing length existing pre-mRNAs via 5' 3' trans-splicing. In trans-splicing, an exogenous template introduced compete with endogenous pre-mRNA,...
Abstract Adaptive immune repertoires are composed by the ensemble of B and T-cell receptors within an individual, reflecting both past current responses. Recent advances in single-cell sequencing enable recovery complete adaptive receptor sequences addition to transcriptional information. Here, we recovered transcriptome repertoire information for polyclonal T follicular helper cells following lymphocytic choriomeningitis virus (LCMV) infection, CD8+ with binding specificity restricted two...
Abstract B cells contribute to the pathogenesis of both cellular- and humoral-mediated central nervous system (CNS) inflammatory diseases through a variety mechanisms. In such conditions, may enter CNS parenchyma local tissue destruction. It remains unexplored, however, how infection autoimmunity drive transcriptional phenotypes, repertoire features, antibody functionality. Here, we profiled from murine models intracranial (i.c.) viral infections autoimmunity. We identified population...
Abstract Motivation The maturation of systems immunology methodologies requires novel and transparent computational frameworks capable integrating diverse data modalities in a reproducible manner. Results Here, we present the ePlatypus ecosystem for immunogenomics analysis, with focus on adaptive immune repertoires single-cell sequencing. is an open-source web-based platform provides programming tutorials integrative database that helps elucidate signatures B T cell clonal selection....
Abstract Adaptive immune repertoires are composed by the ensemble of B and T cell receptors (BCR, TCR) within an individual reflect both past current responses. Recent advances in single-cell sequencing enable recovery complete adaptive receptor sequences addition to transcriptional information. Such high-dimensional datasets molecular quantification clonal selection cells across a wide variety conditions such as infection disease. Due costs, time required for analysis practices academic...
Abstract B cells contribute to the pathogenesis of both cellular- and humoral-mediated central nervous system (CNS) inflammatory diseases through a variety mechanisms. In such conditions, may enter CNS parenchyma local tissue destruction. It remains unexplored, however, how infection autoimmunity drive transcriptional phenotypes, repertoire features, antibody functionality. Here, we profiled from murine models intracranial (i.c.) viral infections autoimmunity. We identified population...
Abstract The maturation of systems immunology methodologies requires novel and transparent computational frameworks capable integrating diverse data modalities in a reproducible manner. Here, we present the ePlatypus ecosystem for immunogenomics analysis, with focus on adaptive immune repertoires single-cell sequencing. is web-based platform provides programming tutorials an integrative database that elucidates selection patterns immunity. Furthermore, links established bioinformatics...