A5061498808- X-ray Diffraction in Crystallography
- Crystallization and Solubility Studies
- Microfluidic and Bio-sensing Technologies
- Microfluidic and Capillary Electrophoresis Applications
- Mass Spectrometry Techniques and Applications
- RNA and protein synthesis mechanisms
- Supramolecular Chemistry and Complexes
- Arsenic contamination and mitigation
- Dye analysis and toxicity
- Plant Virus Research Studies
- Analytical Chemistry and Chromatography
- Insect-Plant Interactions and Control
- Antimicrobial agents and applications
- Plant biochemistry and biosynthesis
- Metal-Organic Frameworks: Synthesis and Applications
- Pesticide and Herbicide Environmental Studies
- Genomics and Phylogenetic Studies
- biodegradable polymer synthesis and properties
- Garlic and Onion Studies
- bioluminescence and chemiluminescence research
- Advanced battery technologies research
- Pesticide Exposure and Toxicity
- Pharmaceutical and Antibiotic Environmental Impacts
- Nanoplatforms for cancer theranostics
- Biofuel production and bioconversion
Texas A&M University
2010-2022
College Station Medical Center
2014
Mitchell Institute
2014
Louisiana State University
2007
Louisiana State University Agricultural Center
2006
University of Tennessee at Knoxville
2002
Porous coordination nanocages covered with alkyne groups are synthesized through judicious selection of the ligand and reaction conditions. The surface functionalization via click azide-terminated polyethylene glycol turns them into water-stable colloids, which exhibit controlled release an anticancer drug 5-fluorouracil.
We have used an infrared laser for desorption of material and ionization by interaction with electrosprayed solvent. Infrared laser-assisted electrospray (IR LADESI) mass spectrometry was the direct analysis water-containing samples under ambient conditions. An ion trap spectrometer modified to include a pulsed Er:YAG at 2.94 µm wavelength coupled into germanium oxide optical fiber atmospheric pressure nanoelectrospray source ionization. Analytes in aqueous solution were placed on stainless...
The development of well-defined polymeric nanoparticles (NPs) as delivery carriers for antimicrobials targeting human infectious diseases requires rational design the polymer template, an efficient synthetic approach, and fundamental understanding developed NPs, e.g., drug loading/release, particle stability, other characteristics. Herein, we evaluated in vitro antimicrobial activity silver-bearing, fully biodegradable functional NPs. A series degradable (dNPs), composed phosphoester...
A novel polyphosphoester (PPE) with vinyl ether side chain functionality was developed as a versatile template for postpolymerization modifications, and its degradability biocompatibility were evaluated. An organo-catalyzed ring-opening polymerization of ethylene glycol ether-pendant cyclic phosphotriester monomer allowed construction poly(ethylene phosphotriester) (PEVEP). This ether-functionalized PPE scaffold coupled hydroxyl- or thiol-containing model small molecules via three different...
Systematic studies of nonsense and sense suppression the original three derivative Methanosarcina mazei PylRS-tRNAPyl pairs cross recognition between codons various tRNAPyl anticodons in Escherichia coli BL21(DE3) cell strain are reported. is orthogonal E. able to induce strong amber when it co-expressed with pyrrolysyl-tRNA synthetase (PylRS) charged a PylRS substrate, Nε-tert-butoxycarbonyl-l-lysine (BocK). Similar to, also can be coupled genetically incorporate BocK at an ochre mutation...
Thirteen novel non-canonical amino acids were synthesized and tested for suppression of an amber codon using a mutant pyrrolysyl-tRNA synthetase–tRNAPylCUA pair.
An aerobic bacterium capable of breaking down the pesticide acephate (O,S-dimethyl acetyl phosphoramidothioic acid) was isolated from activated sludge collected a manufacturing facility. A phylogenetic tree based on 16 S rRNA gene sequence determined that isolate lies within Pseudomonads. The able to grow in presence at concentrations up 80 mM, with maximum growth 40 mM. HPLC and LC-MS/MS analysis spent medium experiments resting cell assay detected accumulation methamidophos acetate,...
Porous coordination cages (PCCs) constructed from a zirconocene‐based cluster have drawn great attention for their high stability and structural tunability. These features enabled these to be utilized wide array of applications. This work demonstrates the flexibility stimulus‐responsive two interconvertible PCCs. The subjects, PCC‐20c (capsular) PCC‐20t (tetrahedral), are same ligand but exhibit different shapes properties. In synthetic study cages, it is found that higher temperatures...
Abstract Acylsugars, specialized metabolites with defense activities, are secreted by trichomes of many solanaceous plants. Several acylsugar metabolic genes (AMGs) remain unknown. We previously reported multiple candidate AMGs. Here, using approaches, we characterized additional First, identified differentially expressed between high- and low-acylsugar-producing F2 plants derived from a cross cultivated tomato (Solanum lycopersicum) wild relative pennellii), which produce acylsugars that...
Strategies for limiting, or reversing, the degradation of air-sensitive, base metal catalysts hydrogen evolution/oxidation reaction on contact with adventitious O2 are guided by nature′s design hydrogenase active sites. The affinity oxygen sulfur and selenium, in [NiFeS]- [NiFeSe]-H2ase, yields oxygenated chalcogens under aerobic conditions, delays irreversible damage at metals maintaining NiFe core structures. To identify controlling features S-site uptake, related Ni(μ-EPhX)(μ-S′N2)Fe (E =...
Separation and detection of individual submicron polystyrene spheres using capillary electrophoresis with laser-light-scattering has been demonstrated. Electrophoretically separated particles were passed through a focused laser beam light scattered from was collected at 90°. Each diameter injected (from 110 to 992 nm) resulted in the observation well-defined migration window containing multiple peaks, each arising by an particle. The time for particular size corresponded well peak population...
We have applied desorption electrospray ionization to aerosol particles. Ions were formed from aerosols by merging suspended dry particles with an of solvent in a modified ion trap mass spectrometer. Dry generated using fluidized bed powder disperser and directed toward the inlet A nanospray source was used create spray droplets at right angle respect aerosol. interaction transferred into Using this method, pure samples caffeine erythromycin analyzed. In addition, commonly available food...
The azinomycins are potent antitumor agents produced by the soil bacterium Streptomyces sahachiroi and contain a novel aziridino[1,2-a]pyrrolidine core; its synthesis involves at least 14 steps. This study reports first reconstitution of N-acetylglutamine semialdehyde formation two enzymes encoded in azinomycin biosynthetic gene cluster. reaction proceeds through an acylphosphate establishes N-acetyl-glutamyl 5-phosphate as intermediates complex biosynthesis moiety.
A simple and reproducible technique for constructing perfectly aligned gaps in fused-silica capillaries has been developed postcolumn reagent addition with capillary electrophoresis. This uses laser ablation the second harmonic of a Nd:YAG (532 nm) at 13.5 mJ/pulse repetition rate 15 Hz to create these gaps. is glued microscope slide positioned focal point cylindrical lens using focused beam from pointer as reference. Gaps 14.0 ± 2.2 μm (n = 33) bore are produced success 94% by 400 pulses....