A5100602077- Enzyme Production and Characterization
- Protein Hydrolysis and Bioactive Peptides
- Advanced biosensing and bioanalysis techniques
- Biosensors and Analytical Detection
- Microbial Metabolites in Food Biotechnology
- Crystallization and Solubility Studies
- Probiotics and Fermented Foods
- Phytase and its Applications
- X-ray Diffraction in Crystallography
- Oral microbiology and periodontitis research
- Aquaculture disease management and microbiota
- Enzyme Catalysis and Immobilization
- Bacteriophages and microbial interactions
- Studies on Chitinases and Chitosanases
- Food composition and properties
- RNA and protein synthesis mechanisms
- Porphyrin and Phthalocyanine Chemistry
- Biofuel production and bioconversion
- Meat and Animal Product Quality
- Collagen: Extraction and Characterization
- RNA Interference and Gene Delivery
- Plant Pathogens and Resistance
- Microbial Metabolic Engineering and Bioproduction
- Insect Utilization and Effects
- Seaweed-derived Bioactive Compounds
China Animal Disease Control Center
2024
Shandong University of Technology
2024
Jiangsu Yanjiang Chemical Resources Development Research Institute (China)
2014-2021
Shanghai Jiao Tong University
2003-2021
Anhui University
2019-2020
Shanxi University
2020
Jiangsu Ocean University
2010-2019
Environment and Plant Protection Research Institute
2019
Chinese Academy of Tropical Agricultural Sciences
2019
China University of Petroleum, Beijing
2005-2017
Enrofloxacin (ENR) is widely used as a synthetic fluoroquinolone antibiotic for disease control in aquatic animals. ENR aptamers were screened this study using the magnetic bead-SELEX method, and graphene oxide fluorescent sensor was developed to detect residues products. Firstly, conjugated amino beads by amidation reaction, then aptamer sequences showing high affinity step SELEX screening method. Finally, after 10 rounds of screening, six candidate with obtained. Among these, ENR-Apt 6...
Ofloxacin (OFL) is widely used in animal husbandry and aquaculture due to its low price broad spectrum of bacterial inhibition, etc. However, it difficult degrade retained animal-derived food products, which are hazardous human health. In this study, a simple efficient method was developed for the detection OFL residues meat products. coupled with amino magnetic beads by an amination reaction as stationary phase. Aptamer AWO-06, showed high affinity specificity OFL, screened using...
Porous starch, known for its large specific surface area due to internal pores, exhibits excellent adsorption capabilities. In this study, we successfully produced porous starch from lotus seeds using dextranase and conducted a comprehensive analysis of morphology, crystalline structure, pasting behavior, characteristics. The enzymatic treatment resulted in the development pore structure on seed (LS) without altering as confirmed by Fourier transform infrared spectroscopy X-ray diffraction....
This study evaluated the ability of a dextranase from marine bacterium Catenovulum sp. (Cadex) to impede formation Streptococcus mutans biofilms, primary pathogen dental caries, one most common human infectious diseases. Cadex was purified 29.6-fold and had specific activity 2309 U/mg protein molecular weight 75 kDa. showed maximum at pH 8.0 40 °C stable temperatures under 30 ranging 5.0 11.0. A metal ion chemical dependency that Mn2+ Sr2+ exerted positive effects on Cadex, whereas Cu2+,...
Vibrio anguillarum is a bacterial pathogen that causes serious damage to aquatic fish, and its rapid detection prevention are critical. DNAzymes DNA-based catalysts with excellent stability. In this study, in vitro selection of was performed using the crude extracellular matrix (CEM) V. Anguillarum as target. Different from previous selections targeting CEM, work used an unmodified DNA library, allowing easier adoption technology. After seven rounds selection, DNAzyme named VAE-2 high...
Peptides possessing antihypertensive attributes via inhibiting the angiotensin-converting enzyme (ACE) were derived through enzymatic degradation of Trichiurus lepturus (ribbonfish) using alkaline protease. The resulting mixture underwent filtration centrifugation, ultrafiltration tubes, and Sephadex G-25 gels. exhibiting ACE-inhibitory properties DPPH free-radical-scavenging abilities isolated subsequently purified LC/MS-MS, leading to identification over 100 peptide components. In silico...
ABSTRACT Parvalbumin is a major allergen in fish. However, there currently no effective and safe way to remove this from In study, protease gene VSP2V‐280 of marine bacteria Virgibacillus sp. SP2 was cloned expressed. The enzyme showed maximum activity at 50°C pH 10.0. Ca 2+ Cu promoted the enzyme. good parvalbumin degradation efficiency Based on gel analysis, when 0.3 mg/mL incubated with (30 U/mL) containing 1 mM for 3 h, removal rate reached 97%. further used Ctenopharyngodon idella ,...
Abstract Porous starch is a type of modified that contains high number tiny micropores. Due to its natural absorbent properties, porous has broad application prospects. Currently, enzymatic hydrolysis predominantly used for production starch, however, the efficiency relatively low. In this study, sweet potato was prepared by combining physical pretreatments with dextranase, and then adsorption properties were investigated. Ultrasonic, moist heat, microwave pretreatment methods used,...
As biological control agents, bacteriophages can both inhibit the pathogenic bacteria and remove bacterial biofilms from seafood. Vibrio cholerae is pathogen of cholera severe infection could lead watery diarrhea even death. The double-layer agar plate method was used to isolate screen V. samples aquaculture water sewage. Purified were examined through genome sequencing, as well morphological characterizations. Among isolated bacteriophages, bacteriophage VC3 found be a long-tailed...
Collagen from a marine resource is believed to have more potential activity in bone tissue engineering and their bioactivity depends on biochemical structural properties. Considering the above concept, pepsin soluble collagen (PSC) acid (ASC) blue shark (Prionace glauca) skin were extracted its osteogenic properties investigated. The hydroxyproline content was higher PSC than ASC purified collagens contained three distinct bands α₁, α2, β dimer. purity of confirmed by RP-HPLC profile...
Helicobacter pylori was first isolated from gastritis patients by Barry J. Marshall and Robin Warren in 1982, more than 90% of duodenal ulcers about 80% gastric are caused H. infection. Most detection methods require sophisticated instruments professional operators, making slow expensive. Therefore, it is critical to develop a simple, fast, highly specific, practical strategy for the pylori. In this study, we used as target select unique aptamers that can be our random ssDNA an initial...