A5067642980- Methane Hydrates and Related Phenomena
- Astro and Planetary Science
- Microbial Community Ecology and Physiology
- Offshore Engineering and Technologies
- Planetary Science and Exploration
- Astronomical Observations and Instrumentation
- Geophysics and Gravity Measurements
- Hydrocarbon exploration and reservoir analysis
- Geological and Geophysical Studies
- Spacecraft and Cryogenic Technologies
- Geomagnetism and Paleomagnetism Studies
- Geology and Paleoclimatology Research
- Geological Modeling and Analysis
- Seismic Imaging and Inversion Techniques
- Atmospheric and Environmental Gas Dynamics
- Solar and Space Plasma Dynamics
- Scientific Research and Discoveries
- Geological formations and processes
- Genomics and Phylogenetic Studies
- Protist diversity and phylogeny
- Astronomy and Astrophysical Research
- Marine Biology and Ecology Research
- Anaerobic Digestion and Biogas Production
- Nuclear Physics and Applications
- Reservoir Engineering and Simulation Methods
Ifremer
2015-2024
Université de Bretagne Occidentale
2012-2023
Centre National de la Recherche Scientifique
2004-2023
International Ocean Discovery Program
2021
Institut Universitaire Européen de la Mer
2004-2020
Laboratoire des Sciences de l'Environnement Marin
2002-2017
Biology of Extremophiles Laboratory
2009-2011
Japan Agency for Marine-Earth Science and Technology
2005
Identifying the function of uncultured microbes in their environments today remains one main challenges for microbial ecologists. In this article, we describe a new method allowing simultaneous analysis identity and function. This is based on visualization oligonucleotide probe-conferred hybridization signal single cells isotopic measurement using high-resolution ion microprobe (NanoSIMS). order to characterize potential method, an containing iodized cytidine was hybridized fixed Escherichia...
Summary In September 2008, an expedition of the RV Urania was devoted to exploration genomic richness deep hypersaline anoxic lakes (DHALs) located in Western part Mediterranean Ridge. Approximately 40 nautical miles SE from Lake, presence lake, which we named Thetis , confirmed by swath bathymetry profiling and through immediate sampling casts. The brine surface Lake is at a depth 3258 m with thickness ∼157 m. Brine composition found be thalassohaline, saturated NaCl total salinity 348‰,...
Abstract Heat flow is estimated at eight sites drilled int the Guaymas Basin, Gulf of California, during International Ocean Discovery Program Expedition 385. The expedition sought to understand thermal regime basin and heat transfer between off‐axis sills intruding organic‐rich sediments floor. distinct sedimentation rates, active tectonics, magmatism make interesting for scientific discoveries. Results show that corrected values range 119–221 mW/m 2 in 257–1003 site a young sill intrusion,...
A novel, strictly anaerobic, methylotrophic marine methanogen, strain SLH33(T), was isolated from deep sediment samples covered by an orange microbial mat collected the Napoli Mud Volcano. Cells of SLH33(T) were Gram-stain-negative, motile, irregular cocci that occurred singly. utilized trimethylamine, dimethylamine, monomethylamine, methanol, betaine, N,N-dimethylethanolamine and choline (N,N,N-trimethylethanolamine) as substrates for growth methanogenesis. The optimal temperature 30 °C;...
Members of the archaeal order
Due to its permanent vertical stratification, the Black Sea is world’s largest aquatic methane reservoir, holding an estimated 96 Tg of methane1. Understanding biogeochemical processes at work in this unique system crucial for evaluating vulnerability reservoir environmental perturbations. Additionally, such knowledge essential assessing potential deep waters as a viable option carbon storage, contributing strategies aimed mitigating greenhouse gas emissions.Below approximately 150...
A novel piezotolerant, mesophilic, facultatively anaerobic, organotrophic, polarly flagellated bacterium (strain LT13a T ) was isolated from a deep sediment layer in the Nankai Trough (Leg 190, Ocean Drilling Program) off coast of Japan. This organism used wide range organic substrates as sole carbon and energy sources: pyruvate, glutamate, succinate, fumarate, lactate, citrate, peptone tryptone. Oxygen, nitrate, ferric iron cystine were electron acceptors. Maximal growth rates observed at...
Culturable bacteria were detected in deep-sea sediment samples collected from the Nankai Trough site 1173 (Ocean Drilling Program, ODP, Leg 190) at 4.15 m below seafloor with 4791 of overlying water. In this deep ocean near surface sediment, mainly fermentative heterotrophs, autotrophic acetogens and sulfate-reducing enriched by using two different non-selective enrichment culture media. bacterial population shifts within marine enrichments monitored denaturating gradient gel electrophoresis...
A piezotolerant, mesophilic, marine lactic acid bacterium (strain LT20 T ) was isolated from a deep sub-seafloor sediment core collected at Nankai Trough, off the coast of Japan. Cells were Gram-positive, rod-shaped, non-sporulating and non-motile. The NaCl concentration range for growth 0–120 g l −1 , with optimum 10–20 . temperature pH 7·0 4–50 °C, 37–40 °C. 7·0–8·0. pressure 0·1 MPa tolerance up to 30 MPa. main cellular phospholipids phosphatidylglycerols (25 %), diphosphatidylglycerols...
Summary Submarine mud volcanoes are a significant source of methane to the atmosphere. The Napoli volcano, situated in brine‐impacted Olimpi Area Eastern Mediterranean Sea, emits mainly biogenic particularly at centre volcano. Temperature gradients support suggestion that is cold volcano with moderate fluid flow rates. Biogeochemical and molecular genetic analyses were carried out assess methanogenic activity rates, pathways diversity hypersaline sediments Methylotrophic methanogenesis was...
The Sonora Margin cold seeps present a seafloor mosaic pattern consisting of different faunal assemblages and microbial mats. To better understand if sedimentary communities reflect this patchy distribution, all major habitats were investigated using four complementary approaches: 16S rRNA 454 pyrosequencing, quantitative polymerase chain reaction, fluorescence in situ hybridization geochemistry analyses. This study reveals that sediments populated by surface show distinct porewater features...
ABSTRACT Our understanding of the genetics species best-studied hyperthermophilic archaea, Pyrococcus spp . , is presently limited by lack suitable genetic tools, such as a stable cloning vector and ability to select individual transformants on plates. Here we describe development reliable host-vector system for archaeon abyssi Shuttle vectors were constructed based endogenous plasmid pGT5 from P. strain GE5 bacterial pLitmus38. As no antibiotic resistance marker currently available spp.,...
Summary The S onora M argin cold seeps present on the seafloor a patchiness pattern of white microbial mats surrounded by polychaete and gastropod beds. These surface assemblages are fuelled abundant organic inputs sedimenting from water column upward‐flowing seep fluids. Elevated density was observed in underlying sediments. A previous study same samples identified anaerobic oxidation methane ( AOM ) as potential dominant archaeal process these sediments, probably catalysed three clades...
Subsurface sediments of the Sonora Margin (Guaymas Basin), located in proximity active cold seep sites were explored. The taxonomic and functional diversity bacterial archaeal communities investigated from 1 to 10 meters below seafloor. Microbial community structure abundance distribution dominant populations assessed using complementary molecular approaches (Ribosomal Intergenic Spacer Analysis, 16S rRNA libraries quantitative PCR with an extensive primers set) correlated comprehensive...
Marine mud volcanoes are geological structures emitting large amounts of methane from their active centres. The Amsterdam volcano (AMV), located in the Anaximander Mountains south Turkey, is characterized by intense seepage produced part methanogens. To date, information about diversity or metabolic pathways used methanogens centres marine limited. (14)C-radiotracer measurements showed that methylamines/methanol, H(2)/CO(2) and acetate were for methanogenesis AMV. Methylotrophic was measured...
ABSTRACT In the Sonora Margin cold seep ecosystems (Gulf of California), sediments underlying microbial mats harbor high biogenic methane concentrations, fueling various communities, such as abundant lineages anaerobic methanotrophs (ANME). However, biodiversity, distribution, and metabolism microorganisms producing this remain poorly understood. study, measurements methanogenesis using radiolabeled dimethylamine, bicarbonate, acetate showed that production in these was mainly dominated by...
In the Guaymas Basin, presence at a few tens of kilometers cold seeps and hydrothermal vents coupled with comparable sedimentary settings depths offer unique opportunity to assess compare microbial community composition these deep-sea ecosystems. The diversity in sediments from three seep two vent assemblages were investigated using high-throughput 16S rRNA-sequencing. Numerous bacterial archaeal lineages detected both sediments. Various potential organic matter degraders (e.g. Chloroflexi,...