The ability of cancer cells to move and invade the surrounding environment is basis local distant metastasis. Cancer cell movement requires dynamic remodeling cytoskeleton membrane controlled by multiprotein complexes including focal adhesion kinase (FAK) or Neural Wiskott-Aldrich Syndrome Protein (N-WASP). We show that 17β-estradiol induces phosphorylation FAK its translocation toward sites where are assembled. This process triggered via a Gα/Gβ protein-dependent, rapid extranuclear...

Estrogens are important regulators of neuronal cell morphology, and this is thought to be critical for gender-specific differences in brain function dysfunction. Dendritic spine formation dependent on actin remodeling by the WASP-family verprolin homologous (WAVE1) protein, which controls polymerization through actin-related protein (Arp)-2/3 complex. Emerging evidence indicates that estrogens effective cytoskeleton various types via rapid, extranuclear signaling mechanisms. We here show...

Progesterone plays a role in breast cancer development and progression but the effects on cell movement or invasion have not been fully explored. In this study, we investigate actions of natural progesterone synthetic progestin medroxyprogesterone acetate (MPA) actin cytoskeleton remodeling invasion. particular, characterize nongenomic signaling cascades implicated these actions. T47-D cells display enhanced horizontal migration three-dimensional matrices presence both progestins. Exposure...

The aim of this study is the identification direct endothelial regulation by androgens testosterone (T) and dihydrotestosterone (DHT). We tested effects T DHT on nitric oxide (NO) synthesis tissue plasminogen activator (t-PA) inhibitor-1 (PAI-1) expression in human cells ovariectomized (OVX) rats. results showed that at physiological concentrations increase NO. This depends a rapid recruitment extracellular-related kinase (ERK) 1/2 phosphatidylinositol 3-OH (PI3K)/Akt cascades, resulting...

While progesterone plays multiple roles in the process of breast development and differentiation, its role cancer is less understood. We have shown previously that progestins stimulate cell migration invasion because activation rapid signaling cascades leading to modifications actin cytoskeleton membrane are required for movement. In this study, we investigated effects on formation focal adhesion (FA) complexes, which provide anchoring sites attachment extracellular matrix during movement...

Estrogen and selective estrogen receptor modulators (SERMs) differentially impact endometrial cell function, however, the biological basis of these differences is not established. Deregulated adhesion to extracellular matrix, movement invasion are related disorders, such as endometriosis or cancer. Remodeling actin cytoskeleton required achieve movement. (ER) regulates membrane remodeling through extra-nuclear signaling cascades. In this article, we show that administration 17beta-estradiol...

Abstract Breast cancer is the most common malignancy in women and appearance of distant metastases produces death 98% cases. The retinoic acid receptor β ( RAR β) not expressed 50% invasive breast carcinoma compared with normal tissue it has been associated lymph node metastasis. Our hypothesis that protein participates metastatic process. T47D MCF 7 cell lines were used to perform viability assay, immunobloting, migration assays, RNA interference immunofluorescence. Administration RA )...

Progestins have actions on the cardiovascular system, which depend structure as well receptor binding characteristics. Drospirenone (DRSP) is a progestin that uniquely interferes with signaling of mineralocorticoid (MR). Hormone therapy containing DRSP results in blood pressure reduction hypertensive post-menopausal women. We describe effects endothelial nitric oxide (NO) synthesis and compare them those progesterone (P) medroxyprogesterone acetate (MPA). In addition, we herein tested...

The endothelial effects of progestogens are poorly investigated. Actin remodeling and cell movement fundamental for function controlled by the actin-binding protein moesin. In this work, we studied progesterone medroxyprogesterone acetate (MPA) on actin remodeling, moesin activation in human cells. Our findings show that MPA trigger a rapid rearrangement, with formation cortical complexes, pseudopodia membrane ruffles. Both receptor (PR)-dependent via non-genomic signaling cascade involving...

Sex steroids play a key role in cell movement and tissue organization. Cell migration requires the integration of events that induce changes structure such as protrusion, polarization traction toward direction migration. These actions are driven by actin remodeling stabilized development adhesion sites to extracellular matrix via transmembrane receptors linked cytoskeleton. Focal kinase (FAK) is non-receptor tyrosine facilitates control turnover focal complexes. In this work, we demonstrated...

To explore the effects of 17β-estradiol (E(2)) on cytoskeletal remodeling and motility endometrial stromal cells (ESC) Ishikawa to characterize role focal adhesion kinase (FAK) in these processes.In vitro study cellular morphology ESC or cells.University research center.Endometrial samples obtained from women requiring biopsies.Treatments with E(2) multiple inhibitors signaling pathways.Activation FAK, actin remodeling, membrane morphology, cell motility, invasion.Estrogen induces a rapid...

Sex steroids are important regulators of neuronal cell morphology, and this is critical for gender differences in brain function dysfunction. Neuronal morphology controlled by multiprotein complexes including moesin (a member the ezrin/radixin/moesin family), focal adhesion kinase (FAK), or Wiskott-Aldrich syndrome protein-family verprolin homologous (WAVE1) protein, controlling dynamic remodeling cytoskeleton membrane. We investigated actions natural progesterone (P) synthetic progestin...

Limited information is available on the effects of progestins breast cancer progression and metastasis. Cell migration invasion are central for these processes, require dynamic cytoskeletal cell membrane rearrangements motility to be enacted.We investigated progesterone (P), medroxyprogesterone acetate (MPA), drospirenone (DRSP) nestorone (NES) alone or with 17beta-estradiol (E2) T47-D we linked some actions regulation actin-regulatory protein, moesin remodeling.Breast horizontal...

Nicotinic acetylcholine receptors (nAChRs) are pentameric neurotransmitter-gated ion channels that mediate synaptic transmission throughout the nervous system in vertebrates and invertebrates. <i>Caenorhabditis elegans</i> is a nonmammalian model for study of parasitic nematodes. Nematode muscle nAChRs considerable interest because they targets anthelmintic drugs. We show single-channel activity <i>C. first time. Our results reveal L1 larval stage (ACh) activates mainly levamisole-sensitive...

Abstract Raloxifene (RAL) is a selective oestrogen receptor modulator (SERM) approved for the prevention and treatment of osteoporosis breast cancer in postmenopausal women. However, little known on effects this SERM cell metastasis, which main cause morbidity death. Cell movement critical local progression distant metastasis cells. These processes rely dynamic control actin cytoskeleton membrane morphology. The aim present study was to characterize RAL or 17β‐estradiol (E2) plus (ER)...

The thyroid hormone triiodothyronine (T3) plays a fundamental role in growth regulation, differentiation, metabolism and cellular movement. These processes are particularly important considering that deregulation of T3 levels could promote abnormal responsiveness mammary epithelial cells, which may lead to the development progression breast cancer (BC). Once cells migrate invade different tissues, BC metastasis is main cause cancer-related death because it difficult revert this multistep...

All-trans retinoic acid (RA), the primary metabolite of vitamin A, controls development and homeostasis organisms tissues. RA its natural synthetic derivatives, both known as retinoids, are promising agents in treating chemopreventing different neoplasias, including breast cancer (BC). Focal adhesion kinase (FAK) is a crucial regulator cell migration, overexpression associated with tumor metastatic behavior. Thus, pharmaceutical FAK inhibitors (FAKi) have been developed to counter action. In...

Somatostatin plays a role in physiological and pathological cell proliferation angiogenesis. Five subtypes of somatostatin receptors have been identified, the therapeutic use receptor-selective agonists has reported several diseases.The aim was to describe expression functional relevance three human (sst1, sst2, sst5) tissues women with without endometriosis.This pilot study analyzed endometrium, ovarian endometriomata, peritoneal lesions 15 patients affected by endometriosis endometrium...