A5016615726- Heat shock proteins research
- Forecasting Techniques and Applications
- Autophagy in Disease and Therapy
- Molecular Biology Techniques and Applications
- RNA modifications and cancer
- Retinoids in leukemia and cellular processes
- Cellular transport and secretion
- Reproductive Biology and Fertility
- Bioinformatics and Genomic Networks
- Protein Structure and Dynamics
- Lysosomal Storage Disorders Research
- Breastfeeding Practices and Influences
- Toxoplasma gondii Research Studies
- Mosquito-borne diseases and control
- Cancer Cells and Metastasis
- Lipid Membrane Structure and Behavior
- Vibrio bacteria research studies
- Epigenetics and DNA Methylation
- Gene expression and cancer classification
- Circadian rhythm and melatonin
- Melanoma and MAPK Pathways
- Seed Germination and Physiology
- Wnt/β-catenin signaling in development and cancer
- Estrogen and related hormone effects
- Plant Reproductive Biology
Consejo Nacional de Investigaciones Científicas y Técnicas
2010-2024
Universidad Nacional de Cuyo
2005-2024
Instituto de Medicina y Biología Experimental de Cuyo
2014-2024
Experimental Medicine and Biology Institute
2023
Centro Científico Tecnológico - Mendoza
2017-2021
Instituto de Histología y Embriología de Mendoza
2007-2010
Pathogens evolved mechanisms to invade host cells and multiply in the cytosol or compositionally functionally customized membrane-bound compartments. Coxiella burnetii, agent of Q fever man is a Gram-negative γ-proteobacterium which multiplies large, acidified, hydrolase-rich fusogenic vacuoles with phagolysosomal-like characteristics. We reported previously that C. burnetii phase II replicative compartments are labelled by LC3, protein specifically localized autophagic vesicles. show here...
Autophagy is an important cellular degradation pathway present in all eukaryotic cells. Via this pathway, portions of the cytoplasm and/or organelles are sequestered double-membrane structures called autophagosomes. In spite significant advance achieved autophagy, long-standing question about source autophagic membrane remains unsolved. We have investigated role secretory autophagosome biogenesis. Sar1 and Rab1b monomeric GTPases that control traffic from endoplasmic reticulum (ER) to Golgi....
Autophagy is the unique, regulated mechanism for degradation of organelles. This intracellular process acts as a prosurvival pathway during cell starvation or stress and also involved in cellular response against specific bacterial infections. Vibrio cholerae noninvasive intestinal pathogen that has been studied extensively causative agent human disease cholera. V. illness produced primarily through expression potent toxin (cholera toxin) within intestine. Besides cholera toxin, this...
ABSTRACT Coxiella burnetii is a Gram-negative obligate intracellular bacterium. After internalization, this bacterium replicates in large parasitophorous vacuole that has features of both phagolysosomes and autophagosomal compartments. We have previously demonstrated early after internalization phagosomes interact with the endocytic autophagic pathways. In report, we present evidence -replicative vacuoles (CRVs) also secretory pathway. Rab1b small GTPase responsible for anterograde transport...
Heat Shock Proteins (HSPs), a family of genes with key roles in proteostasis, have been extensively associated cancer behaviour. However, the HSP is quite large and many its members not investigated breast (BRCA), particularly relation current molecular BRCA classification. In this work, we performed comprehensive transcriptomic study gene patients from both The Cancer Genome Atlas (TCGA) Molecular Taxonomy Breast International Consortium (METABRIC) cohorts discriminating intrinsic subtypes....
We previously reported the association of HSPA1A and HSPB1 with high-grade astrocytomas, suggesting that these proteins might be involved in disease outcome response to treatment. With aim better understand resistance/susceptibility processes associated temozolomide (TMZ) treatment, current study was performed three human malignant glioma cell lines by focusing on several levels: (a) apoptotic index senescence, (b) DNA damage, (c) interaction players damage response. Three lines, Gli36, U87,...
The acrosome reaction is a unique event in the lifespan of sperm characterized by exocytosis acrosomal content and release hybrid vesicles formed patches outer membrane plasma membrane. This regulated mediated essentially same fusion machinery present neuroendocrine cells. However, whereas secretion cells occurs less than second, normally assessed after several minutes incubation with inducers. In this report, we measured kinetics human triggered two stimuli (calcium ionophore progesterone)...
Abstract The oral squamous cell carcinoma (OSCC), which has a high morbidity rate, affects patients worldwide. Changes in SPINK7 precancerous lesions could promote oncogenesis. Our aim was to evaluate as potential molecular biomarker predicts OSCC stages, compared to: HER2, TP53, RB1, NFKB and CYP4B1. This study used biopsies from three patient groups: dysplasia (n = 33), less invasive 28) highly 18). control group consisted of clinically suspicious cases later be confirmed normal mucosa...
All-trans retinoic acid (RA), the primary metabolite of vitamin A, controls development and homeostasis organisms tissues. RA its natural synthetic derivatives, both known as retinoids, are promising agents in treating chemopreventing different neoplasias, including breast cancer (BC). Focal adhesion kinase (FAK) is a crucial regulator cell migration, overexpression associated with tumor metastatic behavior. Thus, pharmaceutical FAK inhibitors (FAKi) have been developed to counter action. In...
We argue that depending on p-values to reject null hypotheses, including a recent call for changing the canonical alpha level statistical significance from .05 .005, is deleterious finding of new discoveries and progress science. Given blanket variable criterion levels both are problematic, it sensible dispense with testing altogether. There alternatives address study design determining sample sizes much more directly than does; but none tools should replace as magic method giving clear-cut...
Accumulated evidence suggests that aberrant methylation of the TP73 gene and increased levels ΔNp73 in primary tumours correlate with poor prognosis. However, little is known regarding transcriptional functional regulation breast cancer. The aim present study was to determine expression isoform, its relationship DNA their clinical prognostic significance cancer patients.TP73 studied TCGA datasets 70 invasive ductal carcinomas (IDCs). p73 isoforms evaluated by immunohistochemistry (IHC)...
We argue that making accept/reject decisions on scientific hypotheses, including a recent call for changing the canonical alpha level from p = .05 to .005, is deleterious finding of new discoveries and progress science. Given blanket variable levels both are problematic, it sensible dispense with significance testing altogether. There alternatives address study design sample size much more directly than does; but none statistical tools should be taken as magic method giving clear-cut...
Desmogleins are involved in cell adhesion conferring structural skin integrity. However, their role inflammation has been barely studied, and whether desmoglein-4 modulates psoriasis lesions is completely unknown. In this study, we assessed the impact of deficiency on severity imiquimod (IMQ)-induced psoriasiform lesions. To end, −/− Oncins France Colony A (OFA) with Sprague–Dawley (SD) genetic background were used. Additionally, human RNA-Seq datasets from (PSO), atopic dermatitis (AD), a...
We argue that making accept/reject decisions on scientific hypotheses, including a recent call for changing the canonical alpha level from p = .05 to .005, is deleterious finding of new discoveries and progress science. Given blanket variable levels both are problematic, it sensible dispense with significance testing altogether. There alternatives address study design sample size much more directly than does; but none statistical tools should be taken as magic method giving clear-cut...
Statistical and machine-learning analyses of tumor transcriptomic profiles offer a powerful resource to gain deeper understanding subtypes disease prognosis. Currently, prognostic gene-expression signatures do not exist for all cancer types, most developed date have been optimized individual types. In Galgo, we implement bi-objective optimization approach that prioritizes gene signature cohesiveness patient survival in parallel, which provides greater power identify phenotypes strongly...
Abstract Breast cancer (BRCA) is a prevalent malignancy with the highest incidence among females. BRCA can be categorized into five intrinsic molecular subtypes (LumA, LumB, HER2, Basal, and Normal), each characterized by varying clinical features determined expression of genes (PAM50). The Heat Shock Protein (HSP) family composed 95 evolutionary conservated, they have critical roles in proteostasis both normal cancerous processes. Many studies linked HSP to development spread cancer. They...
Abstract Aim We evaluated the impact of inpatient and outpatient treatment provided by an infant nutrition foundation in Las Heras, Mendoza, Argentina identified factors that influenced nutritional recovery. Methods This 2010–2018 retrospective study was based on 300 children up to 5 years age with primary malnutrition, who were treated recovery centre, then prevention centre. analysed children's height, weight, psychomotor development living conditions when they admitted, discharged had...
We argue that making accept/reject decisions on scientific hypotheses, including a recent call for changing the canonical alpha level from p = .05 to .005, is deleterious finding of new discoveries and progress science. Given blanket variable levels both are problematic, it sensible dispense with significance testing altogether. There alternatives address study design sample size much more directly than does; but none statistical tools should be taken as magic method giving clear-cut...