Abstract DAB 389 IL‐2 (Denileukin diftitox) is considered an immunotoxin, and it the first immunotoxin approved by Food Drug Administration. It used for treatment of a cutaneous form T‐cell lymphoma. This fusion protein has two disulfide bonds in its structure that play essential role toxicity functionality immunotoxin. Escherichia coli ( E. ) strain BL21 (DE3) not capable making reductive cytoplasm, but Rosetta‐gami proper correct expression due to mutations glutaredoxin reductase...

Randomized, double-blind, controlled trial study.This study aimed to evaluate the safety and efficacy of topical tranexamic acid (TXA) on intraoperative blood loss (IBL) in patients that have degenerative lumbar canal stenosis undergo posterior laminectomy discectomy.The volume IBL is directly proportional potential surgical complications. Recent reports shown use antifibrinolytic drugs, such as TXA, during surgery might decrease improve patient outcomes.A total 104 with were enrolled this...

Many bacteria, including the genus Bacillus, are able to produce proteases (keratinase). In produced in stationary phase and initial stages of sporulation. Protease production is coordinated with sporulation which expression various genes by different sigma factors manages transition from exponential phase. present study sigma-F gene an indigenous Bacillus cereus strain, involved transcription maintaining sporulation, was deleted. The sigma-H gene, whose product activates that function zero...

Background: Human IFN-α is a family of structurally related proteins that exhibit wide range antiproliferative and antiviral activities. IFNs bind to specific cell receptor as the IFN-α/β (IFNAR) compose IFNAR1 IFNAR2 chains. Interferon’s affinity for chains changes type its activity. Objective: To understand structural basis these different activities, some important amino acid residues in this study binding region interferon were replaced more effective ones. Method: In order suggestion...

Background: DAB389IL-2 (Denileukin diftitox) as an immunotoxin is a targeted pharmaceutical protein and the first approved by FDA. It used for treatment of various kinds cancer such CTCL lymphoma, melanoma, Leukemia but among all these, has special importance. consists two distinct parts; catalytic domain Diphtheria Toxin (DT) that genetically fused to whole IL-2. Deamidation most important reaction chemical instability proteins occurs during manufacture storage. asparagine residues at...

Rasouli, Hamid Reza Msc; Khoshmohabat, Hadi MD; Ahmadpour, Fathollah PhD Author Information

Chemotherapy and radiotherapy have negative effects on normal tissues they are very expensive lengthy treatments. These disadvantages recently attracted researchers to the new methods that specifically affect cancerous lower damage tissues. One of these is use intelligent recombinant fusion toxin. The toxin DTGCSF, which consists linked Diphtheria Toxin (DT) Granulocyte Colony Stimulate Factor (GCSF), was first studied by Chadwick et al. in 1993 where HATPL linker provided linking sequence...

Traumatic brain injury (TBI) is the leading cause of death, disability, and mental health disorders. A wide range bioactive lipids, cytokines, chemokines drives inflammatory response. This study aimed to assess efficacy buprenorphine on moderate Trauma Brain Injury (mTBI) in rats.In this study, 21 Wistar male rats weighing 230 ± 10 g were included. We trained cases by Morris water navigation task mTBI induced pendulum. Then, treatment with 0.05 mg per kilogram body weight continued from day...

Patients with multiple traumas are at high risk of developing respiratory complications, including pneumonia and acute distress syndrome. Many pulmonary complications associated systemic inflammation neutrophilic infiltration. Leukotriene-receptor antagonists anti-inflammatory anti-oxidant drugs subsiding airway inflammation. The present study investigates the effectiveness montelukast in reducing among trauma patients.This randomized, double-blind, placebo-control trial was conducted...

This study aimed to evaluate the role of inflammatory blood markers in predicting pathological response rate after neoadjuvant chemoradiation (neo-CRT) patients with locally advanced rectal cancer (LARC).In this prospective cohort study, we analyzed data LARC who underwent neo-CRT and surgical removal mass between 2020 2022 a tertiary medical center. Patients were examined weekly during neutrophil-to-lymphocyte ratio (NLR), platelet-to-lymphocyte (PLR), monocyte-to-lymphocyte (MLR), systemic...