A5101952219- HIV Research and Treatment
- Viral Infections and Outbreaks Research
- Viral Infections and Vectors
- Advanced Biosensing Techniques and Applications
- Bacteriophages and microbial interactions
- Hepatitis B Virus Studies
- Pelvic floor disorders treatments
- Viral Infections and Immunology Research
- Virology and Viral Diseases
- SARS-CoV-2 and COVID-19 Research
- Intimate Partner and Family Violence
- Global Maternal and Child Health
- Monoclonal and Polyclonal Antibodies Research
- Reproductive tract infections research
- Protein purification and stability
- Mosquito-borne diseases and control
- Lipid Membrane Structure and Behavior
- COVID-19 Clinical Research Studies
- Urinary Tract Infections Management
- Plant Virus Research Studies
- HIV/AIDS drug development and treatment
- Long-Term Effects of COVID-19
Public Health Agency of Canada
2021-2024
University of Manitoba
2021-2024
Karolinska Institutet
2016-2018
A temporal increase in non-B subtypes has earlier been described Sweden by us and we hypothesized that this increased viral heterogeneity may become a hotspot for the development of more complex unique recombinant forms (URFs) if epidemics converge. In present study, performed subtyping using four automated tools phylogenetic analysis RAxML pol gene sequences (n = 5246) HIV-1 near full-length genome (HIV-NFLG) 104). CD4+ T-cell decline trajectory algorithm was used to estimate time HIV...
Abstract Ebola virus (EBOV) is able to persist and actively replicate in the reproductive tract of male disease survivors months or years after recovery from (EVD) 1 . Persistent EBOV infections are usually asymptomatic can be transmitted sexually, but host viral factors that mediate these have not been characterized 2,3 We investigated interaction between during infection blood testis barrier (BTB), with a focus on Sertoli cells as potential reservoir for persistence. assessed replication...
Genotypic tropism testing (GTT) for co-receptor usage is a recommended tool clinical practice before administration of the CCR5-antagonist maraviroc. For some isolates, phenotypic (PTT) revealed discordant results with GTT. In this study, we performed comparative study between GTT and PTT in HIV-1C from East Africa (HIV-1CEA) compared data HIV-1B 01_AE described maraviroc susceptibility CCR5-tropic strains. Patient-derived HIV-1 envgp120 region was cloned into modified pNL4-3 plasmid...
<title>Abstract</title> <italic><bold>Background </bold></italic> Routine characterization of clinical samples for their immunological responses against infectious pathogens often involves assessing cytokine/chemokine profiles and/or production pathogen-specific antibodies. To prevent transmission materials in laboratories, these are inactivated by detergents or heat before the molecular assays performed. Antibody-based assays, such as ELISA, highly sensitive to conformational changes...
Abstract Clinical samples are routinely inactivated before molecular assays to prevent pathogen transmission. Antibody-based sensitive changes in analyte conformation, but the impact of inactivation on detectability has been overlooked. This study assessed effects commonly used inactivation-methods, Triton X-100 (0.5%) and heat (60 °C, 1 h), cytokine/chemokine detection plasma, lung aspirates, nasopharyngeal samples. Heat significantly reduced plasma (IL-12p40, IL-15, IL-16, VEGF, IL-7,...
<title>Abstract</title> Ebola virus (EBOV) persists and actively replicates in the reproductive tract of up to 50% male disease survivors six month after infection1. Persistent EBOV infections are usually asymptomatic can be transmitted sexually, but host viral factors that mediate these have not been characterized2,3. We assessed replication kinetics responses mouse testicular Leydig Sertoli cells infected with wild-type Makona (wtEBOV) collected samples 28 days post-infection. While...
Recent outbreaks of Ebola virus linked to chains transmission from the 2014-2016 West African epidemic suggest a new paradigm for persistent infections as lasting concern public health. Cases disease sexual and detection in male reproductive tract long after patients have recovered suggests that persistence occurs this immune privileged area. However, little is known about cell tropism, viral kinetics, host response infection testis. In study, we challenged immunocompromised mice testicular...