A5074890957- Genetics, Aging, and Longevity in Model Organisms
- Genomics and Chromatin Dynamics
- RNA and protein synthesis mechanisms
- RNA Research and Splicing
- Circadian rhythm and melatonin
- FOXO transcription factor regulation
- Heat shock proteins research
- Neuroendocrine regulation and behavior
- Spaceflight effects on biology
- CRISPR and Genetic Engineering
- Photoreceptor and optogenetics research
California Institute of Technology
2016-2021
Howard Hughes Medical Institute
2016-2017
Abstract Enhancers physically interact with transcriptional promoters, looping over distances that can span multiple regulatory elements. Given enhancer–promoter (EP) interactions generally occur via common protein complexes, it is unclear whether EP pairing predominantly deterministic or proximity guided. Here, we present cross-organismic evidence suggesting most pairs are compatible, largely determined by physical rather than specific interactions. By reanalyzing transcriptome datasets,...
Significance Active torque generation in the actin cytoskeleton has been implicated driving left–right symmetry breaking of developing embryos, but which molecules generate active and how is organized subcellularly remain unclear. This study shows that cortical Formin, recruited to regions where RhoA signaling active, promotes actomyosin layer. We find tends locally rotate cortex a clockwise fashion, drives emergence chiral counterrotating flows with consistent handedness facilitates C....
Deep sequencing of size-selected DNase I–treated chromatin (DNase-seq) allows high-resolution measurement accessibility to I cleavage, permitting identification de novo active cis -regulatory modules (CRMs) and individual transcription factor (TF) binding sites. We adapted DNase-seq nuclei isolated from C. elegans embryos L1 arrest larvae generate maps TF binding. Over half embryonic hypersensitive sites (DHSs) were annotated as noncoding, with 24% in intergenic, 12% promoters, 28% introns,...
To mitigate the deleterious effects of temperature increases on cellular organization and proteotoxicity, organisms have developed mechanisms to respond heat stress. In eukaryotes, HSF1 is master regulator shock transcriptional response, but response pathway not yet fully understood. From a forward genetic screen for suppressors heat-shock-induced gene expression in
Enhancers physically interact with transcriptional promoters, looping over distances that can span multiple regulatory elements. Given enhancer-promoter (EP) interactions generally occur via common protein complexes, it is unclear whether EP pairing predominantly deterministic or proximity guided. Here we present cross-organismic evidence suggesting most pairs are compatible, largely determined by physical rather than specific interactions. By re-analyzing transcriptome datasets, find the...
1 Abstract Proper left-right symmetry breaking is essential for animal development and in many species the actin cytoskeleton plays an instrumental role this process. Active torque generation actomyosin layer promotes C. elegans embryos by driving chiral counter-rotating cortical flows. While both Formins Myosins have been implied breaking, can rotate filaments vitro , it remains unclear if active torques cortex are generated Formins, Myosins, or both. We combined strength of genetics with...
Abstract To mitigate the deleterious effects of temperature increases on cellular organization and proteotoxicity, organisms have developed mechanisms to respond heat stress. In eukaryotes, HSF1 is master regulator shock transcriptional response, but response pathway not yet fully understood. From a forward genetic screen for suppressors induced gene expression in C. elegans , we identified new allele hsf-1 that alters its DNA-binding domain, three additional alleles sup-45, previously...
Abstract RNA Polymerase II contains a disordered C-terminal domain (CTD) whose length enigmatically correlates with genome size. The CTD is crucial to eukaryotic transcription, yet the functional and evolutionary relevance of this variation remains unclear. Here, we use smFISH, live imaging, RNA-seq investigate how disorder influence transcription. We find that modulates size frequency transcriptional bursting. Disorder highly conserved mediates CTD-CTD interactions, an ability show...