A5005476102- Ubiquitin and proteasome pathways
- vaccines and immunoinformatics approaches
- Algal biology and biofuel production
- Peptidase Inhibition and Analysis
- Glycosylation and Glycoproteins Research
- interferon and immune responses
- Cellular transport and secretion
- Moringa oleifera research and applications
- Microbial Metabolic Engineering and Bioproduction
- Enzyme Structure and Function
- Toxoplasma gondii Research Studies
- Marine and coastal ecosystems
- Seaweed-derived Bioactive Compounds
- Biochemical Acid Research Studies
- Protein Hydrolysis and Bioactive Peptides
- Aquatic Ecosystems and Phytoplankton Dynamics
- Minerals Flotation and Separation Techniques
- RNA and protein synthesis mechanisms
- Photosynthetic Processes and Mechanisms
- Diatoms and Algae Research
MRC Protein Phosphorylation and Ubiquitylation Unit
2020-2024
University of Dundee
2020-2024
Marine Biological Association of the United Kingdom
2023-2024
Citadel
2024
University of Exeter
2020-2024
Plymouth Marine Laboratory
2019
University of Plymouth
2019
Of the 16 non-structural proteins (Nsps) encoded by SARS CoV-2, Nsp3 is largest and plays important roles in viral life cycle. Being a large, multidomain, transmembrane protein, has been most challenging Nsp to characterize. Encoded within papain-like protease domain (PLpro) that cleaves not only polypeptide but also K48-linked polyubiquitin ubiquitin-like modifier, ISG15, from host cell proteins. We here compare interactors of PLpro find largely overlapping interactome. Intriguingly, we...
Abstract Branched ubiquitin (Ub) chains constitute a sizable fraction of Ub polymers in human cells. Despite their abundance, our understanding branched function cell signaling has been stunted by the absence accessible methods and tools. Here we identify cellular branched-chain-specific binding proteins devise approaches to probe K48–K63-branched function. We establish method monitor cleavage linkages within complex unveil ATXN3 MINDY as debranching enzymes. engineer K48–K63 branch-specific...
Branched ubiquitin (Ub) chains make up a significant proportion of Ub polymers in human cells and are formed when two or more sites on single molecule modified with creating bifurcated architectures. Despite their abundance, we have poor understanding the cellular functions branched signals that stems from lack facile tools methods to study them. Here develop comprehensive pipeline define function, using K48-K63-branched as case study. We discover branch-specific binders and, by developing...
Electro-coagulation floatation (ECF) is a foam-floatation dewatering method that has been shown to be highly effective, rapid, and scalable separation methodology. In this manuscript, an in-depth analysis of the gas flocculant levels observed during process provided, with microbubbles in 5–80 μm size range at concentration 102–103 bubbles mL−1. Electrolysis microalgae culture was then observed, demonstrating both effective using aluminium electrodes (nine microalgal species tested, 1–40...
Seaweeds contain many varied and commercially valuable components, from individual pigments metabolites through to whole biomass, yet they remain an under cultivated underutilised commodity. Currently, commercial exploitation of seaweeds is predominantly limited biomass consumption or single product extracts for the food industry. The development a seaweed biorefinery, based around multiple products services, could provide important opportunity exploit new currently underexplored markets....
Diatoms are a group of silicified algae that play major role in marine and freshwater ecosystems. Diatom chloroplasts were acquired by secondary endosymbiosis exhibit important structural functional differences from the primary plastids land plants green algae. Many functions plastids, including photoacclimation inorganic carbon acquisition, regulated calcium-dependent signalling processes. Calcium has also been implicated photoprotective responses diatoms; however, nature calcium elevations...
Abstract Of the 16 non-structural proteins (Nsps) encoded by SARS CoV-2, Nsp3 is largest and plays important roles in viral life cycle. Being a large, multidomain, transmembrane protein, has been most challenging Nsp to characterize. Encoded within papain-like protease PLpro domain that cleaves not only protein but also polyubiquitin ubiquitin-like modifier ISG15 from host cells. We here compare interactors of find largely overlapping interactome. Intriguingly, we near full length more...
A novel transketolase has been reconstituted from two separate polypeptide chains encoded by a 'split-gene' identified in the genome of hyperthermophilic bacterium, Carboxydothermus hydrogenoformans. The active α2β2 tetrameric enzyme biochemically characterized and its activity determined using range aldehydes including glycolaldehyde, phenylacetaldehyde cyclohexanecarboxaldehyde as ketol acceptor hydroxypyruvate donor. This reaction proceeds to near 100% completion due release product...
Abstract Diatoms are a group of silicified algae that play major role in marine and freshwater ecosystems. Diatom chloroplasts were acquired by secondary endosymbiosis exhibit important structural functional differences from the primary plastids land plants green algae. Many functions plastids, including photoacclimation inorganic carbon acquisition, regulated calcium-dependent signalling processes. Calcium has also been implicated photoprotective responses diatoms, although nature calcium...