A5060841578- Sperm and Testicular Function
- Reproductive Biology and Fertility
- Cellular Mechanics and Interactions
- Computational Drug Discovery Methods
- Chromosomal and Genetic Variations
- Reproductive biology and impacts on aquatic species
- Plant-derived Lignans Synthesis and Bioactivity
- Ubiquitin and proteasome pathways
- Cell Image Analysis Techniques
- Biocrusts and Microbial Ecology
- vaccines and immunoinformatics approaches
- RNA and protein synthesis mechanisms
- Peptidase Inhibition and Analysis
- Reproductive Health and Technologies
- interferon and immune responses
- Animal Genetics and Reproduction
- CRISPR and Genetic Engineering
- Plant Molecular Biology Research
- Traditional and Medicinal Uses of Annonaceae
- Tree Root and Stability Studies
- Carbohydrate Chemistry and Synthesis
- Developmental Biology and Gene Regulation
- Advanced biosensing and bioanalysis techniques
- Long-Term Effects of COVID-19
- Protein Degradation and Inhibitors
National Phenotypic Screening Centre
2019-2024
University of Dundee
2019-2024
Edinburgh College
2024
University of Edinburgh
2024
Max Planck Institute of Molecular Cell Biology and Genetics
2014-2023
Ninewells Hospital
2019-2023
Institute of Molecular Biotechnology
2011-2013
Austrian Academy of Sciences
2011-2013
Freie Universität Berlin
1979
Versuchs- und Lehranstalt für Brauerei in Berlin
1978
The piRNA (PIWI-interacting RNA) pathway is a small RNA silencing system that acts in animal gonads and protects the genome against deleterious influence of transposons. A major bottleneck field lack comprehensive knowledge factors molecular processes constitute this pathway. We conducted an RNAi screen Drosophila identified ~50 genes strongly impact ovarian somatic Many fall into functional categories indicate essential roles for mitochondrial metabolism, export, nuclear pore, transcription...
Of the 16 non-structural proteins (Nsps) encoded by SARS CoV-2, Nsp3 is largest and plays important roles in viral life cycle. Being a large, multidomain, transmembrane protein, has been most challenging Nsp to characterize. Encoded within papain-like protease domain (PLpro) that cleaves not only polypeptide but also K48-linked polyubiquitin ubiquitin-like modifier, ISG15, from host cell proteins. We here compare interactors of PLpro find largely overlapping interactome. Intriguingly, we...
Male contraceptive options and infertility treatments are limited, almost all innovation has been limited to updates medically assisted reproduction protocols methods. To accelerate the development of drugs that can either improve or inhibit fertility, we established a small molecule library as toolbox for assay screening campaigns using human spermatozoa. We have profiled compounds in Sperm Toolbox several automated high-throughput assays measure stimulation inhibition sperm motility...
There is an urgent need to develop new methods for male contraception, however a major barrier drug discovery has been the lack of validated targets and absence effective high-throughput phenotypic screening system. To address this deficit, we developed fully-automated robotic platform that provided quantitative evaluation compound activity against two key attributes human sperm function: motility acrosome reaction. In order accelerate contraceptive development, screened comprehensive...
Can a high-throughput screening (HTS) platform facilitate male fertility drug discovery?
Abstract Of the 16 non-structural proteins (Nsps) encoded by SARS CoV-2, Nsp3 is largest and plays important roles in viral life cycle. Being a large, multidomain, transmembrane protein, has been most challenging Nsp to characterize. Encoded within papain-like protease PLpro domain that cleaves not only protein but also polyubiquitin ubiquitin-like modifier ISG15 from host cells. We here compare interactors of find largely overlapping interactome. Intriguingly, we near full length more...
Abstract How morphogenetic movements are robustly coordinated in space and time is a fundamental open question biology. We study this using the wing of Drosophila melanogaster , an epithelial tissue that undergoes large-scale flows during pupal stages. showed previously (Etournay et al., 2015) morphogenesis involves both cellular behaviors allow relaxation mechanical stress, as well appear to be actively patterned. The core planar cell polarity (PCP) pathway influences many other contexts,...
Abstract Background Multiple studies have reported an increased risk of acute myocardial infarction, cardiac arrhythmia, heart failure, myocarditis, and venous thromboembolism in patients admitted to hospital with COVID-19 infection. Whether the incidence these cardiovascular conditions differ hospitalised compared those who excluded is not clear. Methods In a prospective, multi-centre, cohort study secondary tertiary care hospitals Scotland, consecutive adults symptoms suspected were...
Abstract Male contraceptive options and infertility treatments are limited. To help overcome these limitations we established a small molecule library as toolbox for assay development screening campaigns using human spermatozoa. We have profiled all compounds in our automated high-throughput platform provide the dataset, which allows direct side-by-side comparisons of compound effects assays live
How morphogenetic movements are robustly coordinated in space and time is a fundamental open question biology. We study this using the wing of Drosophila melanogaster , an epithelial tissue that undergoes large-scale flows during pupal stages. Previously, we showed morphogenesis involves both cellular behaviors allow relaxation mechanical stress, as well appear to be actively patterned (Etournay et al., 2015). Here, show these active not guided by core planar cell polarity (PCP) pathway,...
Abstract Calophyllolid ( 1 ) ist als starkes orales Anticoagulans beschrieben worden. 10 Cumarin‐bzw. Naphthopyronderivate wurden dem analoge Teilstrukturen synthetisiert. Keine der Substanzen war in Lage, die plasmatische Blutgerinnung zu hemmen.
Abstract Study question Can a high-throughput screening platform facilitate male fertility drug discovery? Summary answer A identified large number of compounds that enhanced sperm motility. What is known already Several efforts to find small molecules modulating function have been performed but not using technology. design, size, duration Healthy donor semen samples were used and pooled (3-5 donors per pool). Primary was in singlicate; dose-response duplicate (independent pools)....
ABSTRACT We have adapted the cell painting assay developed by Carpenter and colleagues on cultured U2OS cells to human spermatozoa. In Sperm Cell Painting (SCP) we assemble an image-based quantitative fingerprint of functional state sperm. use this gain insight into mechanism action compounds that modify sperm function as a platform for contraceptive discovery.
Abstract There is an urgent need to develop new methods for male contraception, however a major barrier drug discovery has been the lack of validated targets and absence effective high-throughput phenotypic screening system. To address this deficit, we developed fully-automated robotic platform that provided quantitative evaluation compound activity against two key attributes human sperm function: motility acrosome reaction. In order accelerate contraceptive development, screened...