A5035583337- Neuroscience and Neuropharmacology Research
- Epigenetics and DNA Methylation
- Ion channel regulation and function
- Connexins and lens biology
- Genetic and Kidney Cyst Diseases
- Pluripotent Stem Cells Research
- Genetic Syndromes and Imprinting
- Neurogenesis and neuroplasticity mechanisms
- Genetic Associations and Epidemiology
- Zebrafish Biomedical Research Applications
- Microtubule and mitosis dynamics
- Ultrasound in Clinical Applications
- Cancer-related molecular mechanisms research
- Ion Transport and Channel Regulation
- Lipid metabolism and biosynthesis
- Liver Disease Diagnosis and Treatment
- Cancer survivorship and care
- Blood properties and coagulation
- Memory and Neural Mechanisms
- Hippo pathway signaling and YAP/TAZ
- Retinal Development and Disorders
- Axon Guidance and Neuronal Signaling
- Erythrocyte Function and Pathophysiology
- Cell death mechanisms and regulation
- RNA modifications and cancer
University of Maryland, Baltimore
2007-2019
University of Maryland, Baltimore County
2007-2017
Illinois Department of Public Health
2012
University of Toledo Medical Center
2004
The transmembrane 6 superfamily member 2 ( TM6SF2 ) loss‐of‐function variant rs58542926 is a genetic risk factor for nonalcoholic fatty liver disease and progression to fibrosis but paradoxically associated with lower levels of hepatically derived triglyceride‐rich lipoproteins. expressed predominantly in small intestine, sites lipoprotein biogenesis export. In light this, we hypothesized that may exhibit analogous effects on both intestine lipid homeostasis. To test genotyped 983 bariatric...
Shaping of the neural tube, precursor brain and spinal cord, involves narrowing elongation tissue, concomitantly with other morphogenetic changes that contribue to this process. In zebrafish, medial displacement cells (neural convergence or NC), which drives infolding ectoderm, is mediated by polarized migration cell towards dorsal midline. Failure undergo proper NC results in severe tube defects, yet molecular underpinnings process remain poorly understood. We investigated here role...
Abstract The spatial organization of the mouse cerebellum into transverse zones and parasagittal stripes is reflected during temporal progression Purkinje cell death in Lurcher mutant (+/ Lc ). Neurodegeneration +/ apparent by second postnatal week initially seen all four zones: anterior (lobules I–V), central VI, VII), posterior VIII, dorsal IX), nodular (ventral lobule IX X) zone. However, from day (P)25–P36, loss proceeds more rapidly zone, followed zones, significantly delayed Coronal...
Oxidative stress is postulated to play a role in cell death many neurodegenerative diseases. As model of neonatal neuronal death, we have examined the oxidative Purkinje heterozygous Lurcher mutant (+/Lc). gain function mutation delta2 glutamate receptor (GluRdelta2) that turns into leaky membrane channel, resulting chronic depolarization +/Lc cells starting around first week postnatal development. Virtually, all die by end month. To investigate nitric oxide synthase (NOS) activity and...
Alström syndrome (OMIM #203800) is an autosomal recessive obesity ciliopathy caused by loss-of-function mutations in the ALMS1 gene. In addition to multi-organ dysfunction, such as cardiomyopathy, retinal degeneration and renal disorder characterized high rates of obesity, insulin resistance early-onset type 2 diabetes mellitus (T2DM). To investigate underlying mechanisms T2DM phenotypes, we generated a deletion alms1 zebrafish. We demonstrate conservation hallmark clinical characteristics...
The differentiation and survival of heterozygous Lurcher (+/Lc) Purkinje cells in vitro was examined as a model system for studying how chronic ionic stress affects neuronal survival. mutation the delta2 glutamate receptor (GluRdelta2) converts an orphan into membrane channel that constitutively passes inward cation current. In GluRdelta2(+/Lc) mutant, cell dendritic is disrupted degenerate following first week postnatal development. To determine if phenotype recapitulated vitro, +/+, +/Lc...
Elevated levels of low-density lipoprotein cholesterol (LDL-C) are a major risk factor for cardiovascular disease via its contribution to the development and progression atherosclerotic lesions. Although genetic basis LDL-C has been studied extensively, currently known variants account only ≈20% variation in levels.
Adult rats exposed to the DNA-methylating agent methylazoxymethanol on embryonic day 17 show a pattern of neurobiological deficits that model some neuropathological and behavioral changes observed in schizophrenia. Although it is generally assumed these reflect targeted disruption neurogenesis, unknown whether effects generalise other antimitotic agents administered at different stages development. In present study, neurochemical, electrophysiological techniques were used determine exposure...
Abstract Increased LDL-cholesterol (LDL-C) and fibrinogen are independent risk factors for cardiovascular disease (CVD). We identified novel associations between an Amish-enriched missense variant (p.Asn352Ser) in a functional domain of beta-1,4-galactosyltransferase 1 ( B4GALT1 ) 13.5 mg/dl lower LDL-C (p=1.6E-15), 26 plasma (p= 9.8E-05). N-linked glycan profiling found p.Asn352Ser to be associated (p-values from 1.4E-06 1.0E-17) with decreased glycosylation glycoproteins including:...
A common assumption of excitotoxic mechanisms in the nervous system is that ionic imbalance resulting from overstimulation glutamate receptors and increased Na + Ca ++ influx overwhelms cellular energy metabolic systems leading to cell death. The goal this study was examine how a chronic channel leak current developing Purkinje cells heterozygous Lurcher mutant (+/ Lc ) affects expression distribution α 3 subunit /K ATPase pump, key component homeostasis maintains equilibrium neurons....
Microtubules (MTs) are dynamic and fragile structures that challenging to image in vivo, particularly vertebrate embryos. Immunolabeling methods described here analyze distinct populations of MTs the developing neural tube zebrafish embryo. While focus is on tissue, this methodology broadly applicable other tissues. The procedures optimized for early mid-somitogenesis-stage embryos (1 somite 12 somites), however they can be adapted a range stages with relatively minor adjustments. first...
Microtubules (MTs) are dynamic and fragile structures that challenging to image in vivo, particularly vertebrate embryos. Immunolabeling methods described here analyze distinct populations of MTs the developing neural tube zebrafish embryo. While focus is on tissue, this methodology broadly applicable other tissues. The procedures optimized for early mid-somitogenesis-stage embryos (1 somite 12 somites), however they can be adapted a range stages with relatively minor adjustments. first...
Abstract Background: Of the 13.7 million cancer survivors in United States, 22% are breast survivors. By 2050, this number is expected to double. The 2005 Institute of Medicine's Report “From Cancer Patient Survivor: Lost Transition,” identified 4 essential components survivorship care. Central includes coordination between specialists and primary care providers. Primary Care Providers (PCPs) often play a critical role follow-up for patients with chronic diseases. can now be viewed as...
The planar cell polarity (PCP) pathway plays a significant role in facilitating neural convergence (NC) – the narrowing of plate before formation tube. Evidence from literature and our laboratory suggests that NC zebrafish requires elongation midline‐directed polarized migration cells. Failure or delayed stages tube morphogenesis can result severe defects (NTDs) which have been observed all vertebrates studied. Although perturbation PCP is associated with NTDs model organisms humans,...
SCIENTIFIC ABSTRACT Alström syndrome is an autosomal recessive obesity ciliopathy caused by loss-of-function mutations in the ALMS1 gene. In addition to multi-organ dysfunction, such as cardiomyopathy, retinal degeneration, and renal disorder characterized high rates of obesity, insulin resistance early onset type 2 diabetes mellitus (T2DM). To investigate mechanisms linking disease phenotypes we generated a deletion lms1 zebrafish using CRISPR/Cas9. We demonstrate conserved phenotypic...