Routine medical Ultrasound (US) scans are recommended for expectant mothers to monitor the health and growth of fetus. However, in rural areas developing underdeveloped countries face difficulties receiving timely due a lack expertise facilities. Consequently, maternal fetal deaths occur at higher rates these countries, especially first trimester. Novel concepts such as Virtual Doctors, Hospital Home, self- Internet Medical Things (IoMT) may address aforementioned problem effectively....

There is a growing interest for viral vector-free chimeric antigen receptor (CAR) T-cells due to its ability kill cancer cells without adverse side effects. A potential avenue manufacturing viral-vector free CAR utilize mRNA electroporation. One of the major concerns with electroporated shorter cytotoxic lifespan few days, which insufficient or not ideal therapy. To better understand this issue and develop solution, study focused on examining translation molecules, time dependent degradation...

The detection and quantification of nucleic acid proteomic biomarkers in bodily fluids is a critical part many medical screening diagnoses. However, majority the current platforms are not ideal for routine, rapid, low-cost testing point-of-care settings. To address this issue, we developed concept disposable universal biosensor that can detect quantify diluted serum samples. central tenet sensing use dielectrophoresis, electrothermal effects, thermophoresis to selectively rapidly isolate...

Insulator-based dielectrophoretic (iDEP) trapping, separating, and concentrating nanoscale objects is carried out using a nonmetal, unbiased, mobile tip acting as tweezers. The spatial control manipulation of fluorescently labeled polystyrene particles DNA were performed to demonstrate the feasibility iDEP Frequency-dependent tweezers' strength polarity quantitatively determined two theoretical approaches DNA, which resulted in factor 2–40 differences between them. In either approach, was at...

Abstract Electrical impedance based biosensing is a label‐free technique that gaining momentum in biology/medicine. The electrical impedance, typically measured using an array of micro‐fabricated interdigitated electrode (IDE), byproduct the interaction between electric fields and target bio‐molecules/cells. In current biosensing, it has been focused on utilizing magnitude (|Z|) to detect/quantify bio‐molecules. There were no reports designing IDE electrodes, sensitivity analysis detailed...

Recent developments in chimeric antigen receptor (CAR) T-cell therapy for cancer have shown promising results. CAR T-cells are engineered mainly by AC or DC electroporation to express molecules on their surfaces. Electroporation has detrimental effects the majority of and causes cell death via apoptosis necrosis. These non-viable cells potentially harmful other healthy T-cells. Because there no unique biomarkers that pinpoint necrosis T-cells, currently available separation methods incapable...

We developed a new label-free biosensor technique for the detection of messenger ribonucleic acid (mRNA) that can be used in prognosis and diagnosis certain diseases. observed dependence negative dielectrophoresis (DEP) force applied to polystyrene microspheres (PMs) conjugation with different types mRNA frequency electric field produced by interdigitated microelectrodes. Since DEP is an effective transduction mechanism mRNA, this sensing technology has potential identification gene...

Abstract Chimeric antigen receptor (CAR) T-cell therapy is a Food and Drug Administration (FDA)-approved treatment for specific cancers. CAR T-cells are manufactured ex vivo using derived from patients. Recent clinical trials reported very high level of efficacy as well complete remission rates (70%–93%) acute lymphoblastic leukemia (ALL) However, there have also been reports significant adverse effects resulting the contamination patients with existing cancer cells during manufacturing. The...

Nanoelectronic devices integrated with dielectrophoresis (DEP) have been promoted as promising platforms for trapping, separating, and concentrating target biomarkers cancer cells from a complex medium. Here, we visualized DEP gradients in conventional nanoelectronic by using multi-pass atomic force microcopy techniques. Our measurements directly demonstrated short range only at sharp step edges of electrodes, frequency dependent polarity, separation distance strength. Additionally,...

Accurate foetal ultrasound (US) image segmentation facilitates advanced obstetric health care by enabling remote monitoring of expectant mothers. However, US is challenging due to distortions, motion artefacts, various imaging conditions and presence maternal anatomy. Recent research work has proposed many methods towards increasing the accuracy segmentation. This paper reviews 2D 3D under four main categories; deep learning-based method, machine methods, active contour-based...

Diagnosing and treating lung diseases can be challenging since the signs symptoms of a wide range medical conditions indicate interstitial diseases. Respiratory impose an immense worldwide health burden. It is even more deadly when considering COVID-19 in present times. Auscultation most common primary method respiratory disease diagnosis. known to non-expensive, non-invasive, safe, takes less time for However, diagnosis accuracy using auscultation subjective experience knowledge physician,...

Weight loss through dietary and exercise intervention is commonly prescribed but not effective for all individuals. Recent studies have demonstrated that circulating microRNA (miR) biomarkers could potentially be used to identify individuals who will likely lose weight diet attain a healthy body weight. However, accurate detection of miRs in clinical samples difficult, error-prone, expensive. To address this issue, we recently developed iLluminate-a low-cost highly sensitive miR sensor...

Circulating Tumor Cells have recognized as a promising biomarker for detection of cancer. However, there is no robust method to isolate CTCs in low-cost and label free manner. To address this need, we demonstrated multi parameter based cell that applicable CTC.

We report on the development of universal, disposable biomarker detection platform for accurate disease biomarkers within 30 min assay time. This technology could replace current dependent, expensive and less sensitive nucleic acid antigen currently used in screening diagnosis.

We demonstrate a rapid and label-free method to detect the concentration of messenger RNA proteins from living cells that can be used in identification cancer by combining dielelectrophoresis image processing.

Abstract Cell transfection by electroporation is a biological assay that has been utilized to inject exogenous molecules (e.g.: RNA, DNA and protein) into live cells. Recently, in developing cell therapy for cancer (e.g., CAR T-cell). One of the major drawbacks current methods death during process. These dead cells can be detrimental, if injected back patients. Current filtering are unable purify T-cells following electroporation, this due lack unique biomarkers target apoptosis necrosis...

Expressions of circulating microRNA 935 and 140 were evaluated pre- post-8-week diet exercise intervention in overweight subjects using a sensor developed by our group. A unique pre-to-post expression pattern was identified only the who lost 5% or more excess weight.