A5100420455- Lipid metabolism and biosynthesis
- Sperm and Testicular Function
- HIV Research and Treatment
- Immune Cell Function and Interaction
- Atherosclerosis and Cardiovascular Diseases
- Aquaculture disease management and microbiota
- Autophagy in Disease and Therapy
- Genetic Mapping and Diversity in Plants and Animals
- Immune Response and Inflammation
- Zebrafish Biomedical Research Applications
- Cardiac Fibrosis and Remodeling
- Angiogenesis and VEGF in Cancer
- vaccines and immunoinformatics approaches
- Wound Healing and Treatments
- Immunotherapy and Immune Responses
- IL-33, ST2, and ILC Pathways
- Cytokine Signaling Pathways and Interactions
- Research in Cotton Cultivation
- Toxoplasma gondii Research Studies
- Diabetic Foot Ulcer Assessment and Management
- Genetic and phenotypic traits in livestock
- Immunodeficiency and Autoimmune Disorders
- Mechanical Circulatory Support Devices
- TGF-β signaling in diseases
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
Boston Children's Hospital
2022-2025
State Key Laboratory of Food Science and Technology
2024
Harvard University
2022-2024
Jiangnan University
2024
Chinese Academy of Medical Sciences & Peking Union Medical College
2024
Zhongkai University of Agriculture and Engineering
2023
Northwest A&F University
2012-2022
First Affiliated Hospital Zhejiang University
2020
Nanjing Agricultural University
2018-2019
Second Xiangya Hospital of Central South University
2017
Diabetes mellitus can cause impaired and delayed wound healing, leading to lower extremity amputations; however, the mechanisms underlying regulation of vascular endothelial growth factor-dependent (VEGF-dependent) angiogenesis remain unclear. In our study, molecular underpinnings dysfunction in diabetes are investigated, focusing on roles disabled-2 (Dab2) Forkhead box M1 (FOXM1) VEGF receptor 2 (VEGFR2) signaling cell function. Bulk RNA-sequencing analysis identified significant...
HIV-1 entry into cells involves formation of a complex between gp120 the viral envelope glycoprotein (Env), receptor (CD4), and coreceptor. For most strains HIV, this coreceptor is CCR5. Here, we provide evidence that CD4 specifically associated with CCR5 in absence or any other receptor-specific ligand. The amount coimmunoprecipitated was significantly higher than major HIV coreceptor, CXCR4, contrast to CXCR4 CD4–CCR5 coimmunoprecipitation not increased by gp120. interaction probably takes...
ABSTRACT The chemokine receptors CXCR4 and CCR5 are the principal coreceptors for infection of X4 R5 human immunodeficiency virus type 1 (HIV-1) isolates, respectively. Here we report on unexpected observation that removal N-linked glycosylation sites in potentially allows protein to serve as a universal coreceptor both laboratory-adapted primary HIV-1 strains. We hypothesize this alteration unmasks existing common extracellular structures reflecting conserved three-dimensional similarity...
Water temperature is the major ecophysiological factor for fish survival in nature and aquaculture. Compared with many homeotherms, can survive prolonged periods under condition of low temperature. However, metabolic strategies liver a cold environment are still unknown this species. In our present study, adult zebrafish were exposed to or plus starvation analyse morphological characteristics hepatocytes by light microscopy transmission electron (TEM). The livers dissected observed...
Steroidogenesis is an indispensable process that indirectly associated with spermatogenesis in the Leydig cell (LC) to utilize lipid droplets (LDs) are critical maintaining normal testosterone synthesis. The regulation of LD mobilization, known as lipophagy, LC still largely unknown.In present study, Chinese soft-shelled turtle was investigated identify steroidogenic activity and lipophagy during annual reproductive cycle by light microscopy, immunohistochemistry (IHC), immunofluorescence...
ABSTRACT The chemokine receptors CCR5 and CXCR4 were found to function in vivo as the principal coreceptors for M-tropic T-tropic human immunodeficiency virus (HIV) strains, respectively. Since many primary cells express multiple receptors, it was important determine if efficiency of virus-cell fusion is influenced not only by presence appropriate coreceptor (CXCR4 or CCR5) but also levels other expressed same target cells. We that with low medium surface CD4 density, coexpression resulted a...
Abstract Water temperature has a major influence on the host innate immune defence and infectivity of pathogens in ectothermic teleosts. Toll‐like receptors (TLRs) are first well‐characterized that conserved vertebrates. However, little is known about effect variation TLRs fish species. In this study, we used adult zebrafish as research model to investigate water TLRs. Whole genome searches identified 20 TLR homologue genes zebrafish. Multiple sequence alignment protein structure analysis...
C3H/HeJ inbred mice are defective in that they highly resistant to endotoxic shock as compared with normal responder mice. Their B cells and macrophages do not respond significantly when exposed lipopolysaccharide (LPS), whereas from the do. Using a functional assay, we previously isolated cDNA, which encodes for Ran/TC4 GTPase. We now show this gene is mutated mice, accounts their resistance endotoxin stimulation. Sequence analysis of independent mutant Lps(d)/Ran cDNAs splenic reveals...
Smooth muscle cells in major arteries play a crucial role regulating coronary artery disease. Conversion of smooth into other adverse cell types the propels pathogenesis Curtailing plaque buildup by modulating reprograming presents us new opportunity to thwart Here, our report how Epsins, family endocytic adaptor proteins oversee influencing master regulators OCT4 and KLF4. Using single-cell RNA sequencing, we characterized phenotype modulated mouse atherosclerotic found that lacking epsins...
Decidualization is a critical step during embryo implantation that characterized by the differentiation of endometrial stromal cells (ESC) into decidua cells. However, mechanism remains largely unknown. Previously, it has been shown null function homeo box A10 (HOXA10) causes defects in both and decidualization, suggesting HOXA10 signalling pathway likely to be involved uterine decidualization. In present study, we determined expression subcellular distribution its downstream molecule, p57,...
Protein-tyrosine-phosphatase CD45 is exclusively expressed in all nucleated cells of the hematopoietic system but rarely endothelial cells. Interestingly, our recent study indicated that activation endogenous promoter human colony forming (ECFCs) induced expression multiple EndoMT marker genes. However, detailed molecular mechanisms underlying drive and therapeutic potential manipulation atherosclerosis are entirely unknown.
Spermatozoa are known to be stored in the epididymis of Chinese soft-shelled turtle Pelodiscus sinensis for long periods after spermiation from testes, but molecular mechanisms underlying this storage largely unknown. In study, epididymal spermatozoa were investigated determine potential mechanism long-term sperm P. sinensis. Transmission electron microscopy (TEM) and Oil red O staining indicated that unusually large cytoplasmic droplets containing lipid (LDs) attached spermatozoa. However,...
Monoacylglycerol acyltransferase (MGAT3, also known as MOGAT3) catalyzes the synthesis of diacylglycerol (DAG) using 2-monoacylglycerol and fatty acyl coenzyme A. This enzymatic reaction is believed to be an essential rate-limiting step for absorption dietary fat in small intestine. However, similar research bovine MOGAT3 gene lacking. Therefore, this paper, polymorphisms were detected 1145 individuals from five cattle breeds by DNA pooling, PCR–RFLP, sequencing methods. The results showed...
This study investigated the association between vancomycin blood brain barrier penetration and clinical response in patients with postsurgical meningitis.Adult meningitis were recruited. Eligible received 500 mg every 6 h for at least 5 days. On day 3 or 4, cerebrospinal fluid (CSF) simultaneous serum samples obtained to determine CSF minimum concentrations (Cmin), Cmin ratio.Twenty-two (14 men 8 women; mean age of 52.6± 12.1 years) The was 3.63 ± 1.64 mg/L 13.38 5.36 serum, ratio 0.291...
A major goal of efforts to develop a vaccine prevent HIV-1 infection is induction broadly cross-reactive neutralizing antibodies (bcnAb). In previous studies we have demonstrated that did cross-react among multiple primary and laboratory strains HIV-1, but neutralized with limited potency. the present study tested hypothesis immunization envelope glycoproteins (Envs) would result in more potent response. One Env, CM243(N610Q), was selected on basis effects single mutations four gp41...
Abstract Diabetes mellitus can cause impaired and delayed wound healing, leading to lower extremity amputations; however, the mechanisms underlying regulation of vascular endothelial growth factor (VEGF)-dependent angiogenesis remain uncertain could reveal new therapeutic targets. In our study, molecular underpinnings dysfunction in diabetes were investigated, focusing on roles Disabled-2 (Dab2) Forkhead Box M1 (FoxM1) VEGF receptor 2 (VEGFR2) signaling cell (EC) function. Bulk...
<b>Abstract ID 87958</b> <b>Poster Board 264</b> CD45 is a marker of hematopoietic cells, which rarely expressed in endothelial cells (EC). However, recent studies indicated that indispensable driving the endothelial-to-mesenchymal transition (EndMT) following sheep myocardial infarction. EC activation hallmark initiation and atheroma progression; EndMT has also been implicated pathogenesis atherosclerosis. Here, we aim to investigate whether contributes progression atherosclerosis by...