A5091844533- Chromosomal and Genetic Variations
- Genomics and Chromatin Dynamics
- CRISPR and Genetic Engineering
- Bacterial Genetics and Biotechnology
- Antibiotic Resistance in Bacteria
- RNA Research and Splicing
- Advanced Fluorescence Microscopy Techniques
- Nuclear Structure and Function
- Bacterial biofilms and quorum sensing
- Genetics, Aging, and Longevity in Model Organisms
- Leptospirosis research and findings
- Biotin and Related Studies
- Bacteriophages and microbial interactions
- Pluripotent Stem Cells Research
- RNA and protein synthesis mechanisms
- Single-cell and spatial transcriptomics
- RNA Interference and Gene Delivery
- Cancer therapeutics and mechanisms
- Yersinia bacterium, plague, ectoparasites research
- DNA Repair Mechanisms
- Advanced biosensing and bioanalysis techniques
- Vector-borne infectious diseases
- Gene expression and cancer classification
European Molecular Biology Laboratory
2021-2025
European Bioinformatics Institute
2024
European Molecular Biology Laboratory
2018-2024
Institut Pasteur de Montevideo
2014
Research Centre in Biological Chemistry of Córdoba
2011-2013
Consejo Nacional de Investigaciones Científicas y Técnicas
2011-2013
Universidad Nacional de Córdoba
2008-2013
Understanding how the nuclear pore complex (NPC) is assembled of fundamental importance to grasp mechanisms behind its essential function and understand role during evolution eukaryotes
How cells establish the interphase genome organization after mitosis is incompletely understood. Using quantitative and super-resolution microscopy, we show that transition from a Condensin to Cohesin-based occurs dynamically over two hours. While significant fraction of Condensins remains chromatin-bound until early G1, Cohesin-STAG1 its boundary factor CTCF are rapidly imported into daughter nuclei in telophase, immediately bind chromosomes as individual complexes sufficient build first...
How genomic DNA is folded during cell division to form the characteristic rod-shaped mitotic chromosomes essential for faithful genome inheritance a long-standing open question in biology. Here, we use nanoscale tracing single dividing cells directly visualize how 3D fold of changes mitosis at scales from loops entire chromosomes. Our structural analysis reveals scaling minimum 6-8 megabases mitosis. Combined with data-driven modeling and molecular perturbations, can show that very large...
Piwi-interacting RNAs (piRNAs) engage Piwi proteins to suppress transposons and nonself nucleic acids maintain genome integrity are essential for fertility in a variety of organisms. In Caenorhabditis elegans , most piRNA precursors transcribed from two genomic clusters that contain thousands individual transcription units. While few genes have been shown be required biogenesis, the mechanism remains elusive. Here we used functional proteomics approaches identify an upstream sequence complex...
The rapid emergence of drug resistance upon treatment Pseudomonas aeruginosa infections with fluoroquinolones is a serious concern. In this study, we report the effect hypermutability on mutant selection window for ciprofloxacin (CIP) by comparing hypermutator MPAO1 mutS and mutT strains wild-type strain. was shifted to higher CIP concentrations both hypermutators, presenting strain broader in comparison mutation prevention (MPC) determined were increased 2- 4-fold over level, respectively....
Summary The spatial organization of the genome is essential for its functions, including gene expression, DNA replication and repair, as well chromosome segregation. Biomolecular condensates loop extrusion have been proposed principal driving forces that underlie formation chromatin compartments topologically associating domains, respectively. However, whether actual 3D-fold in single cells consistent with these mechanisms has difficult to address situ. Here, we present LoopTrace, a workflow...
nfxB encodes a negative regulator of the mexCD-oprJ genes for drug efflux in opportunistic pathogen Pseudomonas aeruginosa. Inactivating mutations this transcriptional constitute one main mechanisms resistance to ciprofloxacin (Cipr). In work, we evaluated use nfxB/Cipr as new test system study mutation spectra P. The analysis 240 occurring spontaneously wild-type and mutator backgrounds or induced by mutagens showed that offers several advantages compared with other detection systems....
Summary How genomic DNA is folded during cell division to form the characteristic rod-shaped mitotic chromosomes essential for faithful genome inheritance a long-standing open question in biology. Here, we use nanoscale DNA-tracing single dividing cells directly visualize how 3D fold of changes mitosis, at scales from loops entire chromosomes. Our structural analysis reveals scaling minimum 6-8 Mbp mitosis. Combined with data-driven modeling and molecular perturbations, can show that very...
The 8-oxodeoxyguanine (8-oxodG) repair system participates in the prevention and correction of mutations generated by oxidative DNA damage prokaryotes eukaryotes. In this study, we report that Pseudomonas aeruginosa strains deficient mechanism inactivation mutT, mutM mutY genes generate a high frequency cells resistant to antibiotic ciprofloxacin. mutT strain, increase ciprofloxacin resistance achieved at threefold minimal inhibitory concentration was about 1600-fold over wild-type (WT)...
Transposable elements are efficient DNA carriers and thus important tools for transgenesis insertional mutagenesis. However, their poor target sequence specificity constitutes an limitation site-directed applications. The insertion IS608 from Helicobacter pylori recognizes a specific tetranucleotide by base pairing, its choice can be re-programmed changes in the transposon DNA. Here, we present crystal structure of capture complex active conformation, providing complete picture molecular...
Several Leptospira species cause leptospirosis, the most extended zoonosis worldwide. In bacteria, two-component systems constitute key signalling pathways, some of which are involved in pathogenesis. The physiological roles largely unknown, despite identifying several dozens within their genomes. Biochemical confirmation an operative phosphorelaying system has been obtained so far only for Hklep/Rrlep pair. It is known that hklep/rrlep knockout strains biflexa result haem auxotrophy,...
How cells establish the interphase genome organization after mitosis is incompletely understood. Using quantitative and super-resolution microscopy, we show that transition from a Condensin to Cohesin-based occurs dynamically over 2 h. While significant fraction of Condensins remains chromatin-bound until early G1, Cohesin-STAG1 its boundary factor CTCF are rapidly imported into daughter nuclei in telophase, immediately bind chromosomes as individual complexes, sufficient build first TAD...
Summary Understanding how the nuclear pore complex (NPC) assembles is of fundamental importance to grasp mechanisms behind its essential function and understand role during evolution eukaryotes 1–4 . While at least two NPC assembly pathways exist, one exit from mitosis growth in interphase, we currently lack a quantitative map molecular events. Here, use fluorescence correlation spectroscopy (FCS) calibrated live imaging endogenously fluorescently-tagged nucleoporins changes composition...