A5037582502- Virus-based gene therapy research
- Viral gastroenteritis research and epidemiology
- Bacteriophages and microbial interactions
- 14-3-3 protein interactions
- Animal Virus Infections Studies
- Protein Kinase Regulation and GTPase Signaling
- Microtubule and mitosis dynamics
- Cytokine Signaling Pathways and Interactions
- Cancer Mechanisms and Therapy
- Growth Hormone and Insulin-like Growth Factors
Centro Nacional de Microbiologia
2014-2018
Instituto de Salud Carlos III
2014-2018
Instituto de Investigaciones Biomédicas Sols-Morreale
2012
Universidad Autónoma de Madrid
2009
Consejo Superior de Investigaciones Científicas
2009
The functions performed by the concentric shells of multilayered dsRNA viruses require specific protein interactions that can be directly explored through their mechanical properties. We studied stiffness, breaking force, critical strain and fatigue individual Triple, Double Single layered rotavirus (RV) particles. Our results, in combination with Finite Element simulations, demonstrate mechanics external layer provides resistance needed to counteract stringent conditions extracellular...
The infectivity of rotavirus, the main causative agent childhood diarrhea, is dependent on activation extracellular viral particles by trypsin-like proteases in host intestinal lumen. This step entails proteolytic cleavage VP4 spike protein into its mature products, VP8* and VP5*. Previous cryo-electron microscopy (cryo-EM) analysis trypsin-activated showed well-resolved spikes, although no density was identified for spikes uncleaved particles; these data suggested that trypsin triggers...