A5077051406- Sperm and Testicular Function
- Reproductive Biology and Fertility
- Cellular transport and secretion
- Reproductive System and Pregnancy
- Phagocytosis and Immune Regulation
- Immune cells in cancer
- stochastic dynamics and bifurcation
- Microtubule and mitosis dynamics
- Cell Adhesion Molecules Research
- Plant Reproductive Biology
- Parvovirus B19 Infection Studies
- NF-κB Signaling Pathways
- Renal and related cancers
- Immunotherapy and Immune Responses
- Nuclear Structure and Function
- Retinal Development and Disorders
- Hedgehog Signaling Pathway Studies
- Drug Transport and Resistance Mechanisms
- Ovarian function and disorders
- Congenital heart defects research
- Planarian Biology and Electrostimulation
- Pancreatitis Pathology and Treatment
- TGF-β signaling in diseases
- Cell death mechanisms and regulation
- Herpesvirus Infections and Treatments
Osaka University
2022-2025
Kobe University
2019-2023
Tohoku University
2017-2019
Spermatozoa need to undergo an exocytotic event called the acrosome reaction before fusing with eggs. Although calcium ion (Ca 2+ ) is essential for reaction, its molecular mechanism remains unknown. Ferlin a single transmembrane protein multiple Ca -binding C2 domains, and there are six ferlins, dysferlin (DYSF), otoferlin (OTOF), myoferlin (MYOF), fer-1–like 4 (FER1L4), FER1L5, FER1L6, in mammals. Dysf , Otof Myof knockout mice have been generated, each mouse line exhibited membrane fusion...
Abstract Background RIBC1 (RIB43A domain with coiled‐coils 1) and RIBC2 2) are homolog proteins of RIB43a which is localized to microtubules in the cilia flagella unicellular organisms. Cryo‐electron microscopy artificial intelligence studies showed that microtubule inner (MIPs) lumen doublet (DMTs) mouse sperm flagella. However, function mammalian reproduction still unknown. Objective To clarify spermatozoa. Materials methods We generated Ribc1 knockout (KO), Ribc2 KO, double‐knockout (...
Conventional dendritic cells (cDCs) are required for peripheral T cell homeostasis in lymphoid organs, but the molecular mechanism underlying this requirement has remained unclear. We here show that cell–specific CD47-deficient ( Cd47 ΔT ) mice have a markedly reduced number of tissues. Direct interaction with cDCs resulted activation latter cells, which turn induced necroptosis former cells. The deficiency and death expression its receptor signal regulatory protein α on cDCs. development...
Sperm heads contain not only the nucleus but also acrosome which is a distinctive cap-like structure located anterior to and derived from Golgi apparatus. The Associated RAB2 Interactors (GARINs; known as FAM71) protein family shows predominant expression in testis all possess RAB2-binding domain confers binding affinity RAB2, small GTPase that responsible for membrane transport vesicle trafficking. Our previous study showed GARIN1A GARIN1B are important biogenesis indispensable male...
Vasohibin‐2 ( VASH 2) is a homolog of 1, an endothelium‐derived angiogenesis inhibitor. mainly expressed in cancer cells, and has been implicated the progression by inducing tumor growth. Although 2 recently reported to be involved epithelial–mesenchymal transition EMT ), its precise roles are obscure. The aim present study was clarify role cells relation transforming growth factor‐β TGF ‐β) signaling, which major stimulator . Decreased expression ovarian significantly repressed ‐β type I...
Calcium signaling is critical for successful fertilization. In spermatozoa, calcium influx into the sperm flagella mediated by sperm-specific CatSper channel necessary hyperactivated motility and male fertility. a macromolecular complex repeatedly arranged in zigzag rows within four linear nanodomains along flagella. Here, we report that
Abstract Vasohibin‐2 ( VASH 2) is expressed in various cancers and promotes their progression. We recently reported that pancreatic cancer patients with higher 2 expression show poorer prognosis. Herein, we sought to characterize the role of cancer. used LSL ‐Kras G12D ; ‐Trp53 R172H Pdx‐1‐Cre KPC ) mice, a mouse model ductal adenocarcinoma PDAC ), cells isolated from them cells). Knockdown Vash2 did not affect proliferation, but decreased migration. When Vash2‐knockdown were orthotopically...
Calcium signaling is critical for successful fertilization. In spermatozoa, calcium influx into the sperm flagella mediated by specific CatSper channel necessary hyperactivated motility and male fertility. a macromolecular complex repeatedly arranged in zigzag rows within four linear nanodomains along flagella. Here, we report that
Voltage-sensing phosphatase (VSP) exhibits voltage-dependent activity toward phosphoinositides. VSP generates a specialized phosphoinositide environment in mammalian sperm flagellum. However, the voltage-sensing mechanism of spermatozoa is not yet characterized. Here, we found that activated during maturation, indicating electric signals immature are essential. Using heterologous expression system, show property mouse (mVSP). The threshold mVSP approximately −30 mV, which sensitive enough to...
Immunity-related GTPases (IRGs), also known as p47 GTPases, are a family of interferon-inducible proteins that play roles in immunity defense against intracellular pathogens. Although the molecular functions IRGs have been well studied, function member, IRGC1, remains unclear. IRGC1 is unique among because its expression not induced by interferon and it expressed predominantly testis. Further, conserved mammals unlike other IRGs. Here, we knocked out (KO) Irgc1 mice using CRISPR/Cas9 system...
Human herpesvirus 6B (HHV-6B), a T-lymphotropic virus, infects almost exclusively humans. An animal model of HHV-6B has not been available. Here, we report the first to mimic pathogenesis; is based on humanized mice in which human immune cells were engrafted and maintained. For replication, adequate T-cell activation (which becomes susceptible HHV-6B) necessary this murine model. found that an additional transfer mononuclear resulted explosive proliferation activated T cells, could be...
Tumor-associated macrophages (TAMs) are abundant in the tumor microenvironment and considered potential targets for cancer immunotherapy. To examine antitumor effects of agents targeting human TAMs vivo , we here established preclinical xenograft models based on immunodeficient mice that express multiple cytokines have been reconstituted with a immune system by transplantation CD34 + hematopoietic stem progenitor cells (HIS-MITRG mice). HIS-MITRG supported growth both cell line (Raji)–...
Lactate dehydrogenase C (LDHC) is specifically expressed in male germ cells and plays critical roles glycolysis. Glycolysis required to supply energy for sperm motility. Previous studies showed that Ldhc knock-out mice exhibit impaired motility.We established human LDHC knock-in (hLDHC KI) examined whether hLDHC KI can be used assess LDHC-targeting drugs.HLDHC was knocked-in the mouse (mLdhc) allele using CRISPR/Cas9 system. Mating tests, motility examinations with a computer-assisted...
Abstract Background Inhibition of sperm maturation in the epididymis is a promising post‐testicular strategy for short‐acting male contraceptives. It has been shown that ROS1, receptor tyrosine kinase expressed epididymis, essential epididymal differentiation, maturation, and fertility mice. However, it unknown if inhibition ROS1 suppresses reversibly. Objectives Our study aimed to investigate effects inhibitor administration mice on function fertility. Materials Methods We used lorlatinib,...