A5079492544- Microbial metabolism and enzyme function
- Fungal and yeast genetics research
- Biofuel production and bioconversion
- Enzyme Production and Characterization
- Microbial Metabolic Engineering and Bioproduction
- Fermentation and Sensory Analysis
- Liver Disease Diagnosis and Treatment
- Polysaccharides and Plant Cell Walls
- Probiotics and Fermented Foods
- Diet, Metabolism, and Disease
- Osteoarthritis Treatment and Mechanisms
- Knee injuries and reconstruction techniques
- Peroxisome Proliferator-Activated Receptors
- Phytase and its Applications
- Protease and Inhibitor Mechanisms
- Insect-Plant Interactions and Control
- Metabolism and Genetic Disorders
- Polyamine Metabolism and Applications
- Microbial Metabolites in Food Biotechnology
- Metalloenzymes and iron-sulfur proteins
- Cell Adhesion Molecules Research
- Biochemical Acid Research Studies
- Total Knee Arthroplasty Outcomes
- Microbial Community Ecology and Physiology
- Folate and B Vitamins Research
Gifu University
2016-2025
Faculty (United Kingdom)
2025
Institute of Applied Biochemistry
2021
Tokyo University of Agriculture
1996-2020
Gunma University Hospital
2017-2019
Kyoto University
1998-2017
Universitas Gadjah Mada
2016-2017
JA Shizuoka Koseiren ENSHU hospital
2016-2017
Gunma University
2017
Kanazawa University
2011-2015
In the methylotrophic bacterium Methylobacterium extorquens strain AM1, MxaF, a Ca(2+)-dependent methanol dehydrogenase (MDH), is main enzyme catalyzing oxidation during growth on methanol. The genome of AM1 contains another MDH gene homologue, xoxF1, whose function in metabolism has remained unclear. this work, we show that XoxF1 also functions as an and La(3+)-dependent. Despite absence Ca(2+) medium was able to grow presence La(3+). Addition La(3+) increased activity but addition had no...
Introduction In Kimoto -style fermentation, a fermentation starter is produced before the primary brewing process to stabilize fermentation. Nitrate-reducing bacteria, mainly derived from water, produce nitrite, and lactic acid bacteria such as Leuconostoc can proliferate because of their tolerance toward low temperature nutritional requirements. Later, Lactobacillus becomes dominant genus, leading weakly acidic conditions that contribute control yeasts undesired bacterial contaminants....
Methylobacterium species are representative of methylotrophic bacteria. Their genomes usually encode two types methanol dehydrogenases (MDHs): MxaF and XoxF. The former is a Ca2+-dependent enzyme, the latter was recently determined to be lanthanide-dependent enzyme that necessary for expression mxaF. This finding revealed unexpected important roles lanthanides in bacterial methylotrophy. In this study, we performed transcriptome sequencing (RNA-seq) analysis using M. aquaticum strain 22A...
The present study was conducted to screen for psychrophilic yeasts that are able degrade pectin compounds at low temperature, and examine the cold-active pectinolytic enzymes produced by isolated yeasts.Psychrophilic yeasts, which grow on as a sole carbon source, pectinolytic-psychrophilic yeast (PPY) strains PPY-3, 4, 5 6, were from soil Abashiri (Hokkaido, Japan). sequences of 28S rDNA D1/D2 PPY-3 4 indicated taxonomic affiliation Cryptococcus cylindricus Mrakia frigida, respectively,...
Neutral proteinases were partially purified from human intervertebral disc by extraction with guanidine hydrochloride followed Sephadex G-75 column chromatography. They showed gelatinolytic and elastolytic activities at neutral pH. The apparent molecular weights of these enzymes 70 KD 25 KD, the former being complexed form latter. sensitivity to various synthetic inhibitors indicated be serine elastases. Furthermore, they considered leukocyte elastases because anti-leukocyte elastase...
Abstract Acetylcholinesterase (AChE) has been increasingly recognized in plants by indirect evidence of its activity. Here, we report purification and cloning AChE from maize (Zea mays), thus providing to our knowledge the first direct molecule plants. was identified as a mixture disulfide- noncovalently linked 88-kD homodimers consisting 42- 44-kD polypeptides. The hydrolyzed acetylthiocholine propyonylthiocholine, but not S-butyrylthiocholine, AChE-specific inhibitor neostigmine bromide...
The present study was conducted by screening zein-degrading bacteria in an attempt to obtain protease.Soil were screened formation of a clear zone on zein plates. Characterization bacterium indicated taxonomic affiliation Bacillus pumilus, and named MS-1 strain. strain produced two different types extracellular proteases, BPP-A BPP-B. In this study, we purified characterized because it exhibited higher ability hydrolyze than When casein used as the substrate, optimal pH for 11.0. BPP-A,...
Strain 76, which was able to utilize dimethyl disulfide (DMDS) as a sole sulfur source, screened from our microbial collection. It identified Pseudomonas fluorescens by taxonomical characterization and 16S rDNA sequence analysis. does not belong the methylotrophs, because it did grow on DMDS or other C1 compounds carbon degradation repressed in presence of glucose, Na2SO4, nutrient broth. Moreover, showed high resistance growing at concentrations up 9.04 mm. Based these findings, strain 76...
A rod-shaped Gram-staining-negative, non-motile, aerobic and fucoidan-digesting strain, designated TC2(T), was isolated from marine algae collected the coast of Sea Okhotsk at Abashiri, Hokkaido, Japan. The bacterium formed yellow, translucent, circular convex colonies. Comparative 16S rRNA gene sequence analysis indicated that strain belonged to genus Flavobacterium, with highest similarities 97.1 97.3 % type strains Flavobacterium frigidarium, frigoris, limicola psychrolimnae. DNA-DNA...
ABSTRACT The methylotrophic yeast Candida boidinii S2 was found to be able grow on pectin or polygalacturonate as a carbon source. When cells were grown 1% (wt/vol) pectin, C. exhibited induced levels of the pectin-depolymerizing enzymes methylesterase (208 mU/mg protein), lyase (673 mU/mg), pectate and polygalacturonase (3.45 U/mg) two methanol-metabolizing peroxisomal enzymes, alcohol oxidase (0.26 dihydroxyacetone synthase (94 mU/mg). numbers peroxisomes also increased ca. two- threefold...
ABSTRACT The physiological role of dihydroxyacetone synthase (DHAS) in Candida boidinii was evaluated at the molecular level. DAS1 gene, encoding DHAS, cloned from host genome, and regulation its expression by various carbon nitrogen sources analyzed. Western Northern analyses revealed that regulated mainly mRNA regulatory pattern DHAS similar to alcohol oxidase but distinct two other enzymes formaldehyde dissimilation pathway, glutathione-dependent dehydrogenase formate dehydrogenase. gene...