A5066913101- Developmental Biology and Gene Regulation
- Animal Genetics and Reproduction
- Pluripotent Stem Cells Research
- Congenital heart defects research
- Axon Guidance and Neuronal Signaling
- Reproductive Biology and Fertility
- Neurogenesis and neuroplasticity mechanisms
- Pharmacological Effects and Toxicity Studies
- Bone Metabolism and Diseases
- CRISPR and Genetic Engineering
- RNA Research and Splicing
- Wnt/β-catenin signaling in development and cancer
- Molecular Biology Techniques and Applications
- Reproductive System and Pregnancy
- Prenatal Substance Exposure Effects
- RNA Interference and Gene Delivery
- Anesthesia and Neurotoxicity Research
- Cancer-related molecular mechanisms research
- Pregnancy and preeclampsia studies
- Circular RNAs in diseases
- Ubiquitin and proteasome pathways
- TGF-β signaling in diseases
- Genetics and Neurodevelopmental Disorders
- Neuroendocrine regulation and behavior
- Hedgehog Signaling Pathway Studies
UCLouvain
2010-2025
Institut Clinique de la Souris
2007-2022
Inserm
2007-2009
Centre National de la Recherche Scientifique
2009
Centre de Recherche Saint-Antoine
2007
Sorbonne Université
2007
University of Oxford
1997-1998
KU Leuven
1995-1996
Posterior neuropore (PNP) closure coincides with the end of gastrulation, marking primary neurulation and body axis formation. Secondary formation involve differentiation tail bud mesenchyme. Genetic control primary-secondary transition is not understood. We report a detailed analysis gene expression in caudal region day 10 mouse embryos during closure. Embryos were collected at 27–32 somite stage, fixed, processed for whole mount situ hybridisation, subsequently sectioned more analysis....
Human disorders caused by inborn errors of cholesterol biosynthesis are characterized dysmorphogenesis multiple organs. This includes limb malformations that observed at high frequency in some disorders, such as the Smith–Lemli–Opitz syndrome, indicating a pivotal role morphogenesis. Recently, it has been demonstrated can modulate activity Hedgehog proteins, act morphogens to regulate precise patterning many embryonic structures, among which developing limbs. To provide insight functions...
HOX genes play essential roles in patterning the anteroposterior axis of animal embryos and formation various organs. In mammals, there are 39 organized into four clusters (HOXA–D) located on different chromosomes. relationship with their orderly arrangement along chromosomes, these show nested expression patterns which imply that embryonic territories co-express multiple main body axis. Interactomic database entries, as well a handful publications, support some proteins can form homodimers...
Yolk sacs of postimplantation mouse embryos were cultured in a mixture human and rat sera. The central nervous system these normal was studied from the stage 5–9 somites (approximately 8.5 postcoital days) to 20–21 9.5 compared with vivo at same stages. This developmental period covers most neural tube closure, early premigratory differentiation neuroectodermal epithelium, glial commitment population germinative cells. neuronal clements vitro cultivated found be identical corresponding...
Histiotrophic nutrition is essential during the peri-implantation development in rodents, but little known about receptors involved protein and lipid endocytosis derived from endometrium uterine glands. Previous studies suggested that cubilin, a multiligand receptor for vitamin, iron, uptake adult, might be important this process, onset of its expression function not known. In study, we analyzed cubilin pre- early post-implantation rodent embryo tested potential cholesterol uptake. Using...
ABSTRACT Hoxa5 is a member of the Hox gene family, which plays critical roles in successive steps central nervous system formation during embryonic and fetal development. expression adult mouse brain has been reported, suggesting that this may be functionally required after birth. To provide further insight into pattern potential functions brain, we have characterized its neuroanatomical profile from stages to adulthood. While most mapping studies based solely on transcript analysis,...
Abstract BACKGROUND Previously, we showed that prenatal exposure to boric acid (BA), an industrial agent with large production, causes alterations of the axial skeleton in rat embryos, reminiscent homeotic transformations. Indeed, Sprague‐Dawley rats exposed utero BA on gestation day 9 (GD 9) had only six, rather than normal seven, cervical vertebrae. This finding, observed 91% GD 21 fetuses, suggests posterior transformations The present study attempts determine if these skeletal could be...
Homeodomain containing transcription factors of the Hox family play critical roles in patterning anteroposterior embryonic body axis, as well controlling several steps organogenesis. Several proteins have been shown to cooperate with members Pbx for recognition and activation identified target enhancers. contact via a conserved hexapeptide motif. Previous biochemical studies provided evidence that amino acid substitutions sequence Hoxa1 abolish its interaction Pbx. As result, these also...
Hoxa5 is a member of the Hox gene family that plays critical roles in successive steps central nervous system formation during embryonic and fetal development. In mouse, was recently shown to be expressed medulla oblongata pons from stages adulthood. these territories, transcripts are enriched many precerebellar neurons several nuclei involved autonomic functions, while HOXA5 protein detected mainly glutamatergic GABAergic neurons. However, whether functionally required after birth remains...
Conditional gene manipulations in mice are increasingly popular strategies biomedical research. These approaches rely on the production of conditional genetically engineered mutant mouse (GEMM) lines with mutations protein-encoding genes. GEMMs then bred one or several transgenic expressing a site-specific recombinase, most often Cre tissue-specific manner. can only be exploited if available to generate somatic mutations, and thus number has significantly increased over last 15 years. Once...
Paralog factors are considered to ensure the robustness of biological processes by providing redundant activity in cells where they co-expressed. However, specific contribution each factor is frequently underestimated. In developing spinal cord, multiple families transcription successively contribute differentiate an initially homogenous population neural progenitors into a myriad neuronal subsets with distinct molecular, morphological, and functional characteristics. The LIM-homeodomain...
Abstract The embryonic functions of Hox proteins have been extensively investigated in several animal phyla. These transcription factors act as selectors developmental programmes, to govern the morphogenesis multiple structures and organs. However, despite variety morphogenetic processes are involved in, only a limited set their target genes has identified so far. To find additional targets, we used strategy based upon simultaneous overexpression Hoxa2 its cofactors Pbx1 Prep cellular model....