Abstract Previous in vivo studies suggested that a high substratum surface free energy (s.f.e.) and an increased roughness facilitate the supragingival plaque accumulation. It is aim of this clinical trial to explore “relative” effect combination these characteristics on growth. 2 strips, one made fluorethylenepropylene (FEP) other cellulose acetate (CA) (polymers with energies 20 58 erg/cm , respectively) were stuck labial central incisors 16 volunteers. Half each strip was smooth (Ra ± 0.1...

In nine patients with fixed prostheses supported by endosseous titanium implants, 2 abutments (trans-mucosal part of the implant) were replaced either an unused standard abutment or a roughened abutment. After 3 months habitual oral hygiene, plaque samples taken for differential phase-contrast microscopy, DNA probe analysis, and culturing. Supragingivally, rough harbored significantly fewer coccoid micro-organisms (64 us. 81%), which is indicative more mature plaque. Subgingivally,...

The purpose of this study was to examine the change in plaque area over nine days vivo on four materials with different surface free-energies (s.f.e.). Twelve healthy dental students participated a crossover, split-mouth, double-blind study. Supragingival formation recorded nine-day period, materials: fluorethylenepropylene (Teflon) (FEP), parafilm (PAR), cellulose acetate (CA), and enamel (E) s.fe. 20, 26, 57, 88 erg/cm 2 , respectively. Strips made from first three were stuck buccal an...

To date poor treatment options are available for patients with congenital pseudarthrosis of the tibia (CPT), a pediatric orphan disease. In this study we have performed an in silico clinical trial on 200 virtual subjects, generated from previously established model murine bone regeneration, to tackle challenges associated small, patient population. Each subject was simulated receive no and morphogenetic protein (BMP) treatment. We shown that degree severity CPT is significantly reduced BMP...

Objective: One of the early hallmarks osteoarthritis (OA) is a progressive degeneration articular cartilage. Early diagnosis OA-associated cartilage alterations would be beneficial for disease prevention and control, development disease-modifying treatments. However, still hampered by lack quantifiable readouts in preclinical models. Design: In this study, we have shown potency contrast-enhanced nanofocus x-ray computed tomography (CE-nanoCT) to used virtual 3-dimensional (3D) histopathology...

The development of alternatives for autologous bone grafts is a major focus tissue engineering. To produce living bone-forming implants, skeletal stem and progenitor cells (SSPCs) are envisioned as key ingredients. SSPCs can be obtained from different tissues including marrow, adipose tissue, dental pulp, periosteum. Human periosteum-derived (hPDCs) exhibit cell characteristics have well-documented in vivo formation potency. Here, we characterized compared hPDCs derived tibia with...

Background: The present multicenter study aimed to survey the patients' experiences in relation diagnosis and nonsurgical treatment of periodontitis according current routine. Methods: Patients (N = 268) treated for plaque‐related were enrolled two groups: group 1 received primary probing pocket depths (PD 1) and/or scaling root planing (SRP 1); 2 consisted patients who scheduled different centers recall PD/scaling/subgingival instrumentation. Data collection included a full periodontal...

Abstract Objectives: Bone augmentation underneath an occlusive titanium membrane is evaluated in most cases by means of serial histological sections and histomorphometry. Micro‐computed tomography (micro‐CT) a less invasive dynamic technique to measure bone volume animals size that fits into the gantry. The aim present study was evaluate whether latter approach could match histomorphometry assess under membrane. Material methods: Pre‐formed cups were placed on skull 16 rabbits. formation...

Strips of teflon and cellulose acetate were glued to the upper lateral incisors human volunteers in a split mouth, double blind study on influence substratum surface free energy (s.f.e.) supragingival dental plaque accumulation during three day period no oral hygiene. Plaque accumulation, microbial composition s.f.e. microorganisms determined compared developed natural enamel surfaces. Significantly less colonised polymer surfaces (p < 0.002). Streptococcus sanguis I was predominant...

Strips of teflon and cellulose acetate were glued to the upper lateral incisors human volunteers in a split mouth, double blind study on influence substratum surface free energy (s.f.e.) supragingival dental plaque accumulation during three day period no oral hygiene. Plaque accumulation, microbial composition s.f.e. microorganisms determined compared developed natural enamel surfaces. Significantly less colonised polymer surfaces (p < 0.002). Streptococcus sanguis I was predominant...

The repair of large long bone defects requires complex surgical procedures as the loss cannot simply be replaced by autologous grafts due to an insufficient stock human body. Tissue engineering strategies and use Advanced Therapy Medicinal Products (ATMPs) for these reconstructions remain a considerable challenge, in particular since robust outcomes well-defined animal models are lacking. To suitable model treatment sized defects, we developed both skeletally immature mature sheep made close...

Background: This study used a tissue‐engineering approach, which combined autologous periosteal cells with scaffold material, to promote bone augmentation under an occlusive titanium barrier that was placed on the skull of rabbits. Because cell–matrix interaction is key importance in tissue engineering, two different calcium phosphate–based scaffolds were seeded cells. One contained hydroxyapatite, tricalcium phosphate, and collagen; other β‐tricalcium phosphate structure. Methods: The...

Neurofibromatosis type 1 (NF1) is an autosomal dominant disorder affecting approximately in 2,000 newborns. Up to 5% of NF1 patients suffer from pseudarthrosis a long bone (NF1-PA). Current treatments are often unsatisfactory, potentially leading amputation. To gain more insight into the pathogenesis we cultured cells PA tissue and normal-appearing periosteum affected for mutation analysis. were available 13 individuals with NF1. Biallelic inactivation was identified all investigated...

The repair of deep osteochondral joint surface defects represents a significant unmet clinical need. Importantly, untreated lesions lead to high rate osteoarthritis. current strategies these include autograft transplantation or "sandwich" combining bone autografts with autologous chondrocyte implantation, poorly documented long-term outcomes. In this study, we first investigated the capacity juvenile grafts (OCGs) in skeletally mature rats. With regenerative model view, produced new...

The recolonization rate of a tooth surface after perfect cleaning follows an exponential curve with very slow growth during the first 24 h. After 4 days undisturbed plaque formation, more than 30% will be covered by plaque. During night, is up to 50% less day. Plaque pattern was found correlate closely irregularities in surface. A positive correlation exists between and substratum free energy (sfe). On surfaces low sfe bacterial slower strength adhesion on these weaker. Moreover, composition...

The natural occurrence of established Helicobacter species was investigated in the stomachs 65 laboratory rabbits, by means polymerase chain reaction (PCR) (65/65) and histological analysis (51/65). degree inflammation different regions rabbits' stomach evaluated on haematoxylin eosin (HE)-stained slides. Four rabbits were found positive for PCR. Based 16S ribosomal ribonucleic acid (rRNA) sequences, H. canadensis/H. pullorum organisms identified three animals. Bacteria seen merely one slide...

Abstract This clinical investigation examined the effect of a sanguinarine extract on bacterial plaque growth in man. Three different mouthrinses were examined: (i) Veadent® containing 0.03% and 0.2% zinc chloride (test rinse); (ii) vehicle solution all excipients except (control (iii) Hibident® chlorhexidine digluconate formulation (positive control rinse). The trial which 12 dental students participated, was designed as single‐blind, cross‐over study. During 3 experimental periods 18 days,...

Autologous chondrocyte implantation (ACI) involves the application of a suspension into membrane-sealed cartilage defect. Recently, "cell-seeded collagen matrix-supported" ACI has been developed wherein chondrocytes are seeded on biomembrane. This study aimed at preclinically comparing 4 variant techniques in refined goat model: 2 traditional procedures, whereby defect is sealed by periosteal flap or membrane, and cell-seeding methods, with membrane either sutured glued defect.The efficacy...