A5091593187- Mesenchymal stem cell research
- Tissue Engineering and Regenerative Medicine
- Pluripotent Stem Cells Research
- 3D Printing in Biomedical Research
- Immune cells in cancer
- Bone fractures and treatments
- Amyotrophic Lateral Sclerosis Research
- Parkinson's Disease Mechanisms and Treatments
- Cancer Cells and Metastasis
- Muscle Physiology and Disorders
- Cellular Mechanics and Interactions
- Periodontal Regeneration and Treatments
- Cancer, Hypoxia, and Metabolism
- Fibroblast Growth Factor Research
- Neurogenetic and Muscular Disorders Research
- Biomedical and Engineering Education
- Ubiquitin and proteasome pathways
- Single-cell and spatial transcriptomics
- RNA Research and Splicing
KU Leuven
2019-2023
Amyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disease where substantial heterogeneity in clinical presentation urgently requires better stratification of patients for the development drug trials and care. In this study we explored through crowdsourcing approach, DREAM Prize4Life ALS Stratification Challenge. Using data from >10,000 1479 community-based patient registers, more than 30 teams developed new approaches machine learning clustering, outperforming best current...
Abstract Cell populations and their interplay provide the basis of a cell-based regenerative construct. Serum-free preconditioning can overcome less predictable behavior serum expanded progenitor cells, but underlying mechanism how this is reflected in vivo remains unknown. Herein, cellular molecular changes associated with phenotype shift induced by serum-free human periosteum-derived cells were investigated. Following BMP-2 stimulation, preconditioned displayed enhanced bone forming...
The development of alternatives for autologous bone grafts is a major focus tissue engineering. To produce living bone-forming implants, skeletal stem and progenitor cells (SSPCs) are envisioned as key ingredients. SSPCs can be obtained from different tissues including marrow, adipose tissue, dental pulp, periosteum. Human periosteum-derived (hPDCs) exhibit cell characteristics have well-documented in vivo formation potency. Here, we characterized compared hPDCs derived tibia with...
Abstract The recent growth of single-cell transcriptomics has turned RNA sequencing (scRNA-seq) into a near-routine experiment. Breakthroughs in improving scalability have led to the creation organism-wide transcriptomic datasets, aiming comprehensively profile cell types and states within an organism throughout its lifecycle. To date, however, skeleton remains majorly underrepresented organ system atlases. Considering how not only serves as central framework vertebrate body but is also home...
Bone fracture healing is a well-orchestrated but complex process that involves numerous regulations at different scales. This complexity becomes particularly evident during the inflammatory stage, as immune cells invade region and trigger cascade of signals to promote favorable regenerative environment. Thus, emergence criticalities this stage might hinder rest process. Therefore, investigation many interactions regulate inflammation has primary importance on exploration overall progression....
The field of tissue engineering aspires to provide clinically relevant solutions for patients through the integration developmental principles with a bottom-up manufacturing approach. However, cell-based advanced therapy medicinal products is hampered by protocol complexity, lack non-invasive critical quality controls, and dependency on animal-derived components differentiation. We investigate serum-free, chemically defined, xeno- lipid-free chondrogenic differentiation medium generate...
Abstract Controlled myogenic differentiation is crucial for developmental formation, homeostatic maintenance and adult repair of skeletal muscle relies on cell fate determinants in progenitors or resident stem cells. Proliferating migrate, adopt spindle shape, align membranes fuse into multinuclear syncytia. These processes are accompanied by cyto-architectural changes driven rearranging cytoskeletal components such as actin microtubules. Here we highlight septins, the fourth component...