A5066506340- 3D Printing in Biomedical Research
- Bone Tissue Engineering Materials
- Cancer Cells and Metastasis
- Osteoarthritis Treatment and Mechanisms
- Cell Image Analysis Techniques
- Collagen: Extraction and Characterization
- Medical and Biological Sciences
- Viral Infectious Diseases and Gene Expression in Insects
- Tissue Engineering and Regenerative Medicine
- Cancer Genomics and Diagnostics
- Pluripotent Stem Cells Research
KU Leuven
2022-2025
Abstract The use of magnetic‐driven strategies for non‐contact manipulation engineered living modules opens up new possibilities tissue engineering. integration magnetic nanoparticles (MNPs) with cartilaginous microtissues enables model‐driven 4D bottom‐up biofabrication remotely actuated assembloids, providing unique properties to mechanoresponsive tissues, particularly skeletal constructs. However, clinical use, the long‐term effects stimulation on phenotype and in vivo functionality need...
Abstract Automated technologies are attractive for enhancing the robust manufacturing of tissue-engineered products clinical translation. In this work, we present an automation strategy using a robotics platform media changes, and imaging cartilaginous microtissues cultured in static microwell platforms. We use automated image analysis pipeline to extract microtissue displacements morphological features as noninvasive quality attributes. As result, empty microwells were identified with 96%...
The field of tissue engineering aspires to provide clinically relevant solutions for patients through the integration developmental principles with a bottom-up manufacturing approach. However, cell-based advanced therapy medicinal products is hampered by protocol complexity, lack non-invasive critical quality controls, and dependency on animal-derived components differentiation. We investigate serum-free, chemically defined, xeno- lipid-free chondrogenic differentiation medium generate...
Abstract Cartilage microtissues are promising tissue modules for bottom up biofabrication of implants leading to bone defect regeneration. Hitherto, most the protocols development these cartilaginous have been carried out in static setups, however, achieving higher scales, dynamic process needs be investigated. In present study, we explored impact suspension culture on cartilage a novel stirred microbioreactor system. To study effect shear stress, experiments with three different impeller...
Abstract Automated technologies are attractive for enhancing a robust manufacturing of tissue engineered products clinical translation. In this work, we present an automation strategy using robotics platform media changes cartilaginous microtissues cultured in static microwell platforms. We use automated image analysis pipeline to extract microtissue displacements and morphological features, which serve as input statistical factor analysis. To minimize displacement suspension leading...