A5037824743- Microbial Natural Products and Biosynthesis
- Genomics and Phylogenetic Studies
- RNA and protein synthesis mechanisms
- Phytochemistry and biological activities of Ficus species
- Bacterial Genetics and Biotechnology
- Biochemical and Molecular Research
- Enzyme-mediated dye degradation
- Bacteriophages and microbial interactions
- Venomous Animal Envenomation and Studies
- Phytochemistry and Bioactive Compounds
- Alkaline Phosphatase Research Studies
- Supramolecular Self-Assembly in Materials
- Protein Structure and Dynamics
- Alzheimer's disease research and treatments
- RNA modifications and cancer
- Insect Pest Control Strategies
- Proteoglycans and glycosaminoglycans research
- Enzyme Catalysis and Immobilization
- Phytochemical compounds biological activities
- Enzyme Production and Characterization
- Innovative Microfluidic and Catalytic Techniques Innovation
- Agriculture and Rural Development Research
- Vascular Tumors and Angiosarcomas
- African Botany and Ecology Studies
- Ion channel regulation and function
Rega Institute for Medical Research
2017-2025
KU Leuven
2017-2025
VIB-KU Leuven Center for Brain & Disease Research
2023-2025
National Research Centre
2013-2025
Ahfad University for Women
2023
Weill Cornell Medical College in Qatar
2016
In this study, mango seed kernels extract contained a considerable amount of phenolics and flavonoids (17,400 3325 mg/100 g seed, respectively). The HPLC profiling revealed that hesperidin was the major phenolic compound extract. This is first report find in extracts. compounds were effective scavenging free radicals DPPH ABTS with IC50 values 47.3 7.9 μg/ml, respectively. total antioxidant activity based on reduction molybdenum also measured. potentially inhibited protease, fibrinogenase,...
Abstract Deposition of amyloid-β (Aβ) peptides in the brain is a hallmark Alzheimer’s disease. Aβs are generated through sequential proteolysis amyloid precursor protein by γ-secretase complexes (GSECs). Aβ peptide length, modulated Presenilin (PSEN) and APH-1 subunits GSEC, critical for pathogenesis. Despite high relevance, mechanistic understanding Aβ, its modulation APH-1, remain incomplete. Here, we report cryo-EM structures human GSEC (PSEN1/APH-1B) reconstituted into lipid nanodiscs...
The gene encoding a thermostable cellulase of family 12 was previously isolated from Rhodothermus marinus through functional screening. CelA is protein 260 aminoacyl residues with 28-residue amino-terminal signal peptide. Mature poorly synthesized in some Escherichia coli strains and not at all others. Here we present an alternative approach for its heterologous production as secreted polypeptide Streptomyces.CelA successfully over-expressed Streptomyces lividans TK24. To this end, fused...
Filamentous organisms of the genus Streptomyces play an important role in industrial production processes, due to their extensive secondary metabolism variability, as well ability secrete efficiently large amounts (heterologous) proteins. While genetic engineering tools are available rapidly build up strain libraries, subsequent screening and bioprocess development still constitutes a bottleneck. This is lack reliable parallelized accelerated cultivation techniques for morphologically...
Ficus carica is one of the most popular and edible plants. Its trees emanate latex high medical importance. The well-studied procoagulant effect ficin a hallmark this which protrudes an interesting question how can plant control effect? In present work, we purified characterized serine protease (FPIII) with fibrinolytic activity from F. study anticoagulant character latex. FPIII was inhibited by PMSF its molecular weight 48 kDa. optimum pH temperature were detected at 8.5 60 °C,...
Peroxidase from sycamore fig Ficus sycomorus latex (POLI) was purified by heat treatment, anion exchange chromatography and molecular exclusion chromatography. The purity determined high specific activity (9166 units/mg protein), purification fold (28), RZ value 3.1 a single band in native polyacrylamide gel electrophoresis (PAGE), sodium dodecyl sulfate (SDS)-PAGE visualized peroxidase on the PAGE. POLI had mass of 43 kDa. Substrates commonly used immunodiagnostic kits as 2,2-azino-bis...
The aim of this study was to compare the potential Ficus sycomorus latex peroxidase (POL) and horseradish (HRP) in decolorization a wide spectrum eight synthetic dyes two natural dyes, hibiscus flower color pomegranate juice. We for first time enzymatically. highest percent reported at 20 mg/l all treated with POL HRP. Both enzymes had lower % azo-carmin (30–33%). During treatment, both titan yellow formed precipitates which settled down were removed by centrifugation. enhancement most...
Gram positive Streptomyces bacteria are profuse secretors of polypeptides using complex, yet unknown mechanisms. Many their secretory proteins proteases that play important roles in the acquisition amino acids from environment. Other regulate cellular proteostasis. To begin dissecting possible role secretion, we applied a multi-omics approach. We probed 190 lividans strain TK24 protein secretion defined media at different stages growth. Transcriptomics analysis revealed transcripts for 93%...
Alternative sigma factors control numerous aspects of bacterial life, including adaptation to physiological stresses, morphological development, persistence states and virulence. This is especially true for the physiologically complex actinobacteria. Here we report development a robust gene deletions system Streptomyces lividans TK24 based on BAC library combined with λ-Red recombination technique. The developed was validated by systematically deleting most highly expressed genes encoding...
Protein secretion is a central biological process in all organisms. Most studies dissecting bacterial mechanisms have focused on Gram-negative cell envelopes such as that of
Fluorescent proteins are a major cell biology tool to analyze protein sub-cellular topology. Here we have applied this technology study secretion in the Gram-positive bacterium Streptomyces lividans TK24, widely used host for heterologous biotechnology. Green and monomeric red fluorescent were fused behind Sec (SPSec) or Tat (SPTat) signal peptides direct them through respective export pathway. Significant of eGFP mRFP was observed exclusively pathways, respectively. Plasmid over-expression...
In view of various peroxidase applications, the searching for new sources unique properties is highly desirable. The present study aims to evaluate efficiency locally grown sycamore latex (POL) conjugation with antibodies and conjugate optimal conditions, storage stability, affinity toward different substrates as compared commercial horseradish (HRP).Anti-mouse were prepared in rabbits purified by protein A sepharose column chromatography. POL HRP conjugates one-step glutaraldehyde coupling...
Two l-amino acid oxidase enzyme isoforms, Cc-LAAOI and Cc-LAAOII were purified to apparent homogeneity from
Abstract Background S. lividans TK24 is a popular host for the production of small molecules and secretion heterologous protein. Within its large genome, twenty-nine non-essential clusters direct biosynthesis secondary metabolites. We had previously constructed ten chassis strains, carrying deletions in various combinations specialized metabolites biosynthetic clusters, such as those blue actinorhodin ( act ), calcium-dependent antibiotic cda undecylprodigiosin red coelimycin A cpk ) melanin...
Streptomyces lividans TK24 is a relevant Gram-positive soil inhabiting bacterium and one of the model organisms genus . It known for its potential to produce secondary metabolites, antibiotics, other industrially products. S. plasmid-free derivative 66 close genetic relative strain coelicolor A3(2). In this study, we used transcriptome proteome data improve annotation genome. The RNA-seq primary 5′-ends transcripts were determine transcription start sites (TSS) in We identified 5,424 TSS,...
Abstract Deposition of amyloid-β (Aβ) peptides in the brain is a hallmark Alzheimer’s disease. Aβs are generated through sequential proteolysis amyloid precursor protein by γ-secretase complexes (GSECs). Aβ peptide length, which modulated Presenilin (PSEN) and APH-1 subunits GSEC, critical for pathogenesis. Despite high relevance, mechanistic understanding Aβ, its modulation APH-1, remain incomplete. Here, we report cryo-EM structures human GSEC (PSEN1/APH-1B) reconstituted into lipid...
The increasing problem of bacterial resistance to antibiotics underscores the urgent need for new antibacterials. Protein export pathways are attractive potential targets. Sec pathway is essential viability and includes components that absent from eukaryotes. Here, we used a high-throughput in vivo screen based on secretion activity alkaline phosphatase (PhoA), Sec-dependent secreted enzyme becomes active periplasm. assay was optimized luminescence-based substrate ~240K small molecule...
Abstract The increasing problem of bacterial resistance to antibiotics underscores the urgent need for new antibacterials. Sec preprotein export pathway is an attractive potential alternative target. It essential viability and includes components that are absent from eukaryotes. Here we used a high throughput in vivo screen based on secretion activity alkaline phosphatase (PhoA), Sec-dependent secreted enzyme becomes active periplasm. assay was optimized luminescence-based substrate ~240K...
Introduction: Angiosarcomas are rare and malignant neoplasms that involve abnormal proliferation migration of cancerous endothelial cells. can arise in any region the body, but tend to be found skin, soft tissue, liver. They associated with high mortality rate due their aggressiveness metastasis. Therapeutic strategies for treating angiosarcomas use cytotoxic drugs radiotherapy. However, patient-resistance these approaches is commonly reported. Hence, there a need further understand...
Abstract S. lividans TK24 is a popular host for the production of small molecules and secretion heterologous proteins. has large genome with at least 29 secondary metabolite gene clusters that are non-essential viability undergo complex regulation. To optimize protein secretion, we previously constructed ten chassis strains devoid several clusters. Genome reduction was aimed reducing carbon flow to metabolites pigmentation in spent growth medium improving colony morphology. Strains...