A5059604181- Fibroblast Growth Factor Research
- Pancreatic function and diabetes
- Erythrocyte Function and Pathophysiology
- Neuroblastoma Research and Treatments
- Cancer, Hypoxia, and Metabolism
Heidelberg University
2019-2020
Heidelberg University
2019
Abstract FGF2 is a tumor cell survival factor that exported from cells by an ER/Golgi-independent secretory pathway. This unconventional mechanism of protein secretion based on direct translocation across the plasma membrane. The Na,K-ATPase has previously been shown to play role in this process, however, underlying remained elusive. Here, we define structural elements are critical for physical interaction between and α1 subunit Na,K-ATPase. In intact cells, corresponding mutant forms were...
Fibroblast growth factor 2 (FGF2) is a cell survival with crucial functions in tumor‐induced angiogenesis. Here, we describe novel time‐resolved FGF2 signaling assay based upon live imaging of neuroblastoma cells. To validate this system, tested 8960 small molecules for inhibition kinetic resolution. Hit compounds were validated dose‐response experiments signaling, FGF receptor antagonism, downstream ERK phosphorylation and FGF2‐dependent chemoresistance cellular leukemia model system. The...
Summary Fibroblast Growth Factor 2 (FGF2) is a tumor cell survival factor that exported from cells by an unconventional secretory pathway. This process based on direct translocation of FGF2 across the plasma membrane. membrane depends PI(4,5)P -induced formation membrane-inserted oligomers followed extracellular trapping at outer leaflet mediated surface heparan sulfate proteoglycans. Beyond well-characterized core mechanism translocation, Na,K-ATPase has been proposed to play so far unknown...