A5032617648- Dementia and Cognitive Impairment Research
- Marine Sponges and Natural Products
- Neuroendocrine regulation and behavior
- Stress Responses and Cortisol
- Atherosclerosis and Cardiovascular Diseases
- Single-cell and spatial transcriptomics
- Balance, Gait, and Falls Prevention
- Adipose Tissue and Metabolism
- Coral and Marine Ecosystems Studies
- Neurobiology of Language and Bilingualism
- Stroke Rehabilitation and Recovery
- Epilepsy research and treatment
- EEG and Brain-Computer Interfaces
- Lower Extremity Biomechanics and Pathologies
- Immune cells in cancer
- Kruppel-like factors research
- Neuroscience and Neuropharmacology Research
- Sports Performance and Training
- Metabolomics and Mass Spectrometry Studies
- Maternal Mental Health During Pregnancy and Postpartum
- Advanced biosensing and bioanalysis techniques
- Regulation of Appetite and Obesity
- Functional Brain Connectivity Studies
- Echinoderm biology and ecology
- Plant Gene Expression Analysis
Georgia Institute of Technology
2013-2025
The Wallace H. Coulter Department of Biomedical Engineering
2013-2025
Emory University
2013-2025
Sogang University
2021-2024
Ulsan National Institute of Science and Technology
2024
Emory and Henry College
2023
Seoul National University
2018-2019
University of Florida
2018
Columbus Oncology and Hematology Associates
2018
University of Utah
2017
Maintenance of vascular integrity in the adult animal is needed for survival, and it critically dependent on endothelial lining, which controls barrier function, blood fluidity, flow dynamics. However, nodal regulators that coordinate identity function remain poorly characterized. Here, we show KLF2 KLF4 control a large segment transcriptome, thereby affecting virtually all key functions. Inducible endothelial-specific deletion Klf2 and/or Klf4 reveals single allele either gene sufficient...
Disturbed flow (d-flow) induces atherosclerosis by regulating gene expression in endothelial cells (ECs). For further mechanistic understanding, we carried out a single-cell RNA sequencing (scRNA-seq) and scATAC-seq study using endothelial-enriched single from the left- right carotid artery exposed to d-flow (LCA) stable-flow (s-flow RCA) mouse partial ligation (PCL) model. We find eight EC clusters along with immune cells, fibroblasts, smooth muscle cells. Analyses of marker genes,...
Studying the role of a particular gene in atherosclerosis typically requires time-consuming and often difficult process generating double knockouts or transgenics on ApoE-/- LDL receptor (LDLR)-/- background. Recently, it was reported that adeno-associated-virus-8 (AAV8)-mediated overexpression PCSK9 (AAV8-PCSK9) rapidly induced hyperlipidemia. However, using this method C57BL6 wild-type (C57) mice, took ~3 months to develop atherosclerosis. Our partial carotid ligation model is used by...
Atherosclerotic plaques exhibit high deposition of cholesterol and macrophages. These are not only the main components but also key inflammation-triggering sources. However, no existing therapeutics can achieve effective removal both within plaques. Here, we report cargo-switching nanoparticles (CSNP) that physicochemically designed to bind release anti-inflammatory drug in plaque microenvironment. CSNP have a core-shell structure with core composed an inclusion complex methyl-β-cyclodextrin...
In order to develop new anti-photoaging agents, we examined the inhibitory effects of 29 seaweed extracts on transcriptional activities NF-κB and AP-1, MMP-1 expression. The from 3 species Alariaceae, Eisenia bicyclis, Ecklonia cava stolonifera, have showed strong inhibition both AP-1 reporter activity, which were well correlated with their abilities inhibit addition, expression was dramatically attenuated by treatment eckol or dieckol purely isolated E. indicating that these compounds are...
Atherosclerosis is an inflammatory disease with multiple underlying metabolic and physical risk factors. Bone morphogenic protein 4 (BMP4) expression increased in endothelium atherosclerosis-prone regions known to induce endothelial inflammation, dysfunction, hypertension. BMP actions are mediated by 2 different types of receptors (BMPRI BMPRII). Here, we show a surprising finding that loss BMPRII causes inflammation atherosclerosis.Using siRNA BMPRII(+/-) mice, found specific knockdown...
Abstract Endothelial cells (ECs) are an important target for therapy in a wide range of diseases, most notably atherosclerosis. Developing efficient nanoparticle (NP) systems that deliver RNA interference (RNAi) drugs specifically to dysfunctional ECs vivo modulate their gene expression remains challenge. To date, several lipid‐based NPs developed and shown RNAi ECs, but few them optimized endothelium. Here, novel, targeted poly( β ‐amino ester) (pBAE) NP is demonstrated. This pBAE...
Atherosclerosis preferentially occurs in arterial regions exposed to disturbed blood flow ( d-flow ), while stable s-flow ) are protected. The proatherogenic and atheroprotective effects of mediated part by the global changes endothelial cell (EC) gene expression, which regulates dysfunction, inflammation, atherosclerosis. Previously, we identified kallikrein-related peptidase 10 Klk10 , a secreted serine protease) as flow-sensitive mouse ECs, but its role biology atherosclerosis was...
Atherosclerosis preferentially occurs in arterial regions of disturbed blood flow, and stable flow (s-flow) protects against atherosclerosis by incompletely understood mechanisms.
Arterial stiffness and wall shear stress are powerful determinants of cardiovascular health, arterial is associated with increased mortality. Low oscillatory stress, termed disturbed flow (d-flow), promotes atherosclerotic remodeling, but the relationship between d-flow not well understood. The objective this study was to define role on stiffening discover relevant signaling pathways by which stiffens arteries.D-flow induced in carotid arteries young old mice both sexes. quantified ex vivo...
Disturbed blood flow (d-flow) occurring in branched and curved arteries promotes endothelial dysfunction atherosclerosis, part, by altering gene expression epigenomic profiles cells. While a systemic metabolic change is known to play role it unclear whether can be regulated local d-flow. Here, we tested this hypothesis carrying out metabolomics study using plasma samples obtained from ApoE −/− mice that underwent partial carotid ligation surgery induce Mice receiving sham were used as...
To evaluate the prognostic outcome of cardiac computed tomography (CT) for prediction major adverse events (MACEs) in low-risk patients suspected having coronary artery disease (CAD) and to explore differential values calcium (CAC) scoring CT angiography.Institutional review committee approval informed consent were obtained. In 4338 who underwent 64-section evaluation CAD, both CAC angiography concurrently performed by using standard scanning protocols. Follow-up clinical data regarding...
DNA barcoding has been widely used in species identification and biodiversity research. A short fragment of the mitochondrial cytochrome c oxidase subunit I (COI) sequence serves as a bio-barcode. We collected barcodes, based on COI sequences from 156 (529 sequences) fish, insects, shellfish. present results phylogenetic relationships to assess Korean peninsula. Average GC% contents 68 fish (46.9%), 59 shellfish (38.0%), 29 insect (33.2%) are reported. Using Kimura 2 parameter all possible...
Background Oxidative stress occurs with disturbed blood flow, inflammation and cardiovascular disease (CVD), yet free-radical scavenging antioxidants have shown limited benefit in human CVD. Thioredoxin-1 (Trx1) is a thiol antioxidant protecting against non-radical oxidants by controlling protein thiol/disulfide status; Trx1 translocates from cytoplasm to cell nuclei due signaling, facilitates DNA binding of transcription factors, e.g., NF-κB, potentiates inflammatory signaling. Whether...
The main research topic in this paper is how to compare multiple biological experiments using transcriptome data, where each experiment measured and designed control treated samples. Comparison of usually performed terms the number DEGs an arbitrary combination experiments. This process facilitated with Venn diagram but there are several issues when used analyze DEGs. First, current tools do not provide systematic analysis prioritize genes. Because that generally fully focus genes, genes...