A5044908145- Neonatal Respiratory Health Research
- Dementia and Cognitive Impairment Research
- Alzheimer's disease research and treatments
- MicroRNA in disease regulation
- Neonatal and fetal brain pathology
- Lipid metabolism and disorders
- Kruppel-like factors research
- Advanced biosensing and bioanalysis techniques
- Traumatic Brain Injury and Neurovascular Disturbances
- Multiple Sclerosis Research Studies
- Cancer-related molecular mechanisms research
- Neuroinflammation and Neurodegeneration Mechanisms
- Coronary Interventions and Diagnostics
- Clinical practice guidelines implementation
- Biomarkers in Disease Mechanisms
- Sepsis Diagnosis and Treatment
- Peripheral Neuropathies and Disorders
- RNA and protein synthesis mechanisms
- RNA Interference and Gene Delivery
- Caveolin-1 and cellular processes
- Hereditary Neurological Disorders
- Hippo pathway signaling and YAP/TAZ
- Epigenetics and DNA Methylation
- Blood properties and coagulation
- Biotin and Related Studies
Meso Scale Discovery (United States)
2023-2025
The Wallace H. Coulter Department of Biomedical Engineering
2020-2023
Georgia Institute of Technology
2020-2023
Demos
2020-2023
Abstract A growing literature suggests that plasma levels of tau phosphorylated at amino acid 217 (pTau217) performs similarly to cerebrospinal fluid (CSF) biomarkers and PET imaging detect amyloid pathology provide diagnostic prognostic information in Alzheimer’s disease (AD), but a significant limiting factor thus far has been lack widely available immunoassays. We evaluated novel pTau217 S-PLEX® assay developed by Meso Scale Discovery (MSD; Rockville, MD) from 131 individuals with AD...
The Alzheimer's Association Global Biomarker Standardization Consortium conducted a blinded case-control study to learn which phosphorylated tau (p-tau) assays provide the largest fold-changes in disease (AD) versus non-AD and show commutability measuring patient samples candidate certified reference materials (CRMs). Thirty-three different p-tau measured paired plasma cerebrospinal fluid (CSF) from 40 participants (25 with "AD pathology" 15 "non-AD by CSF amyloid beta [Aβ]42/Aβ40 p-tau181...
Atherosclerosis preferentially occurs in arterial regions of disturbed blood flow, and stable flow (s-flow) protects against atherosclerosis by incompletely understood mechanisms.
BACKGROUND: Phosphorylated tau (p-tau) is a specific blood biomarker for Alzheimers disease (AD) pathology. Multiple p-tau biomarkers on several analytical platforms are poised clinical use. The Association Global Biomarker Standardisation Consortium plasma phospho-tau Round Robin study engaged assay developers in blinded case-control p-tau, aiming to learn which assays provide the largest fold-changes AD compared non-AD, have strongest relationship between and cerebrospinal fluid (CSF),...
Abstract Background Certain demyelinating disorders, such as neuromyelitis optica spectrum disorder (NMOSD) and myelin oligodendrocyte glycoprotein antibody-associated disease (MOGAD) exhibit serum autoantibodies against aquaporin-4 (αAQP4) (αMOG). The variability of the autoantibody presentation warrants further research into subtyping each case. Methods To elucidate relationship between astroglial neuronal protein concentrations in peripheral circulation with occurrence these...
Atherosclerosis is a chronic inflammatory disease and occurs preferentially in arterial regions exposed to disturbed blood flow (d-flow) while the stable (s-flow) are spared. D-flow induces endothelial inflammation atherosclerosis by regulating gene expression partly through flow-sensitive transcription factors (FSTFs). Most FSTFs, including well-known Kruppel-like KLF2 KLF4, have been identified from vitro studies using cultured cells (ECs). Since many genes pathways lost or dysregulated...
POLDIP2 is a multifunctional protein whose roles are only partially understood. Our laboratory previously reported physiological studies performed using mouse gene trap model, which suffered from three limitations: perinatal lethality in homozygotes, constitutive Poldip2 inactivation and inadvertent downregulation of the adjacent Tmem199 gene. To overcome these limitations, we developed new conditional floxed model. The first part present study shows that our initial mice were affected by an...
ABSTRACT Introduction Screening and treatment of seizures (Sz) in neonates suffering from hypoxic-ischemic encephalopathy (HIE) is routine. Understanding if Sz worsen brain injury outcomes would optimize decisions, recognizing risks benefits. We hypothesized that serum central nervous system (CNS)-specific biomarkers discriminate with relate to outcomes. Methods This retrospective cohort study was conducted between April 28, 2009 November 15, 2019, including diagnosed and/or HIE treated...
Abstract Background Three blood‐based biomarkers of neurological injury—glial fibrillary acidic protein (GFAP), neurofilament light (Nf‐L), and Tau—have emerged as promising disorders injuries such hypoxic‐ischemic encephalopathy (HIE), traumatic brain injury, Alzheimer’s disease (AD). The low levels GFAP, Nf‐L, Tau in serum plasma require highly sensitive assays to detect them. Here, we report the analytical validation an ultrasensitive, electrochemiluminescence‐based, multiplexed...
Abstract Background Neurofilament light chain (NfL) is a key biomarker for axonal injury providing diagnostic and prognostic value in many neurodegenerative diseases. The sensitivity of electrochemiluminescence (ECL) immunoassays has previously not been adequate to quantify plasma NfL levels Alzheimer’s disease (AD). Here we performed clinical evaluation recently developed ultra‐sensitive ECL S‐PLEX ® immunoassay individuals with AD other Method We measured banked samples from 541...
Abstract Background Certain demyelinating disorders, such as neuromyelitis optica spectrum disorder (NMOSD) and myelin oligodendrocyte glycoprotein antibody-associated disease (MOGAD) exhibit serum autoantibodies against aquaporin-4 (αAQP4) (αMOG). The variability of the autoantibody presentation warrants further research into subtyping each case. Methods To elucidate relationship between astroglial neuronal protein concentrations in peripheral circulation with occurrence these...