A5031078585- SARS-CoV-2 and COVID-19 Research
- SARS-CoV-2 detection and testing
- Respiratory viral infections research
- COVID-19 Clinical Research Studies
- Alzheimer's disease research and treatments
- Dementia and Cognitive Impairment Research
- Kawasaki Disease and Coronary Complications
- Cancer, Lipids, and Metabolism
- Mechanical Circulatory Support Devices
- COVID-19 and Mental Health
- S100 Proteins and Annexins
- Glioma Diagnosis and Treatment
- Animal Disease Management and Epidemiology
- RNA modifications and cancer
- Medical and Biological Ozone Research
- COVID-19 diagnosis using AI
- Neonatal and fetal brain pathology
- Health Systems, Economic Evaluations, Quality of Life
- Intensive Care Unit Cognitive Disorders
- Venous Thromboembolism Diagnosis and Management
- Advanced biosensing and bioanalysis techniques
- Dermatological and COVID-19 studies
- Biotin and Related Studies
- Biosensors and Analytical Detection
- Aortic Disease and Treatment Approaches
Meso Scale Discovery (United States)
2021-2025
Stanford Health Care
2024
Stanford University
2024
The Alzheimer's Association Global Biomarker Standardization Consortium conducted a blinded case-control study to learn which phosphorylated tau (p-tau) assays provide the largest fold-changes in disease (AD) versus non-AD and show commutability measuring patient samples candidate certified reference materials (CRMs). Thirty-three different p-tau measured paired plasma cerebrospinal fluid (CSF) from 40 participants (25 with "AD pathology" 15 "non-AD by CSF amyloid beta [Aβ]42/Aβ40 p-tau181...
BACKGROUND: Phosphorylated tau (p-tau) is a specific blood biomarker for Alzheimers disease (AD) pathology. Multiple p-tau biomarkers on several analytical platforms are poised clinical use. The Association Global Biomarker Standardisation Consortium plasma phospho-tau Round Robin study engaged assay developers in blinded case-control p-tau, aiming to learn which assays provide the largest fold-changes AD compared non-AD, have strongest relationship between and cerebrospinal fluid (CSF),...
Detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) antigens in blood has high sensitivity adults with disease 2019 (COVID-19), but pediatric patients is unclear. Recent data suggest that persistent SARS-CoV-2 spike antigenemia may contribute to multisystem inflammatory children (MIS-C). We quantified nucleocapsid (N) and (S) either COVID-19 or MIS-C using ultrasensitive immunoassays (Meso Scale Discovery).Plasma was collected from inpatients (<21 years) enrolled across...
Gliomas are aggressive malignant tumors, with poor prognosis. There is an unmet need for the discovery of new, non-invasive biomarkers differential diagnosis, prognosis, and management brain tumors. Our objective to validate four plasma - glial fibrillary acidic protein (GFAP), neurofilament light (NEFL), matrix metalloprotease 3 (MMP3) fatty acid binding 4 (FABP4) compare them established tumor molecular markers survival.
Abstract Objectives Widespread SARS-CoV-2 testing is invaluable for identifying asymptomatic/pre-symptomatic individuals. There remains a technological gap highly reliable, easy, and quick diagnostic tests suitable frequent mass testing. Compared to nasopharyngeal (NP) swab-based tests, saliva-based methods are attractive due easier safer sampling. Current rapid antigen (RATs) hindered by limited analytical sensitivity. Here, we report one of the first ultrasensitive, assays with an...
Abstract Background To investigate evidence of residual viral infection, intrathecal immune activation, central nervous system (CNS) injury, and humoral responses in cerebrospinal fluid (CSF) plasma patients recovering from coronavirus disease 2019 (COVID-19), with or without neurocognitive post-COVID condition (PCC). Methods Thirty-one participants (25 PCC) underwent clinical examination, lumbar puncture, venipuncture ≥3 months after COVID-19 symptom onset. Healthy volunteers were included....
Convenient and widespread serology testing may alter the trajectory of COVID-19 pandemic. This study seeks to leverage high-throughput, multiplexed serologic assays, which have been adopted as benchmarks for vaccine efficacy, support large-scale surveys SARS-CoV-2 immunity using finger-stick blood and/or saliva. Specifically, we optimized MSD's were analytically validated serum, test self-collected saliva samples identify prior infection. We show that these assays can be used with...
In this two-center prospective cohort study of children on ECMO, we assessed a panel plasma brain injury biomarkers using exploratory factor analysis (EFA) to evaluate their interplay and association with outcomes. Biomarker concentrations were measured daily for the first 3 days ECMO support in 95 participants. Unfavorable composite outcome was defined as in-hospital mortality or discharge Pediatric Cerebral Performance Category > 2 decline ≥ 1 point from baseline. EFA grouped 11 into three...
An increased risk for human infection with avian influenza A(H5N1) viruses is of concern. We developed an internally controlled, dual-target reverse transcription PCR A(H5) subtyping. This test could be used to detect in clinical samples.
We sought to determine if higher plasma levels of brain injury biomarkers neurofilament light (NfL), phosphorylated tau 181 (pT181), tau, and ubiquitin C-terminal hydrolase L1 (UCHL1) were associated with unfavorable outcomes in children supported on extracorporeal membrane oxygenation (ECMO) without preceding cardiac arrest. conducted a secondary analysis two-center prospective observational study ECMO patients 0-<18 years. Plasma concentrations NfL, pT181, UCHL1 measured days 1, 2 3....
Serology provides tools for epidemiologic studies, and may have a role in vaccine prioritization selection. Automated serologic testing of saliva, especially specimens that are self-collected at home sent to laboratory via the mail without refrigeration, could be highly-scalable strategy population-wide testing. In this prospective study, non-vaccinated patients were recruited after PCR self-collect saliva return their mail. Longitudinal analyzed order monitor seroconversion weeks diagnostic...
Abstract Background Detection of SARS-CoV-2 antigens in blood has high sensitivity adults with acute COVID-19, but pediatric patients is unclear. Recent data suggest that persistent spike antigenemia may contribute to multisystem inflammatory syndrome children (MIS-C). We quantified nucleocapsid (N) and (S) either COVID-19 or MIS-C using ultrasensitive immunoassays (Meso Scale Discovery). Methods Plasma was collected from inpatients (<21 years) enrolled across 15 hospitals US states....
<title>Abstract</title> <bold>Background</bold>: Gliomas are aggressive malignant tumors, with poor prognosis. There is an unmet need for the discovery of new, non-invasive biomarkers differential diagnosis, prognosis, and management brain tumors. Our objective to validate four plasma – glial fibrillary acidic protein (GFAP), neurofilament light (NEFL), matrix metalloprotease 3 (MMP3) fatty acid binding 4 (FABP4) compare them established tumor molecular markers survival....
Abstract Background Neurofilament light chain (NfL) is a key biomarker for axonal injury providing diagnostic and prognostic value in many neurodegenerative diseases. The sensitivity of electrochemiluminescence (ECL) immunoassays has previously not been adequate to quantify plasma NfL levels Alzheimer’s disease (AD). Here we performed clinical evaluation recently developed ultra‐sensitive ECL S‐PLEX ® immunoassay individuals with AD other Method We measured banked samples from 541...
Abstract Background There are numerous benefits to performing salivary serology measurements for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the causative pathogen disease 2019 (COVID-19). Here, we used a sensitive multiplex assay quantitate IgG against 4 SARS-CoV-2 antigens: nucleocapsid, receptor-binding domain, spike, and N-terminal domain. Methods We single samples from 90 individuals with COVID-19 diagnosis collected at 0 42 days postsymptom onset (PSO) 15 uninfected...
Abstract Convenient and widespread serology testing may alter the trajectory of COVID-19 pandemic. This study seeks to leverage high-throughput, multiplexed serologic assays, which have been adopted as benchmarks for vaccine efficacy, support large-scale surveys SARS-CoV-2 immunity using finger-stick blood and/or saliva. Specifically, we optimized MSD’s were analytically validated serum, test self-collected saliva samples. We show that these assays can be used with FDA-registered specimen...
Abstract Serology provides tools for epidemiologic studies, and may have a role in vaccine prioritization selection. Automated serologic testing of saliva, especially specimens that are self-collected at home sent to laboratory via the mail without refrigeration, could be highly-scalable strategy population-wide testing. In this prospective study, patients were recruited after PCR self-collect saliva return their mail. Longitudinal analyzed order monitor seroconversion weeks diagnostic test...