A5026653746- Cancer, Lipids, and Metabolism
- 3D Printing in Biomedical Research
- Nanoplatforms for cancer theranostics
- Nanoparticle-Based Drug Delivery
- Cancer Cells and Metastasis
- Cancer-related cognitive impairment studies
- Cellular Mechanics and Interactions
- Reproductive Biology and Fertility
- Brain Metastases and Treatment
- RNA Interference and Gene Delivery
- Sperm and Testicular Function
- Glioma Diagnosis and Treatment
- Ovarian function and disorders
- Prostate Cancer Treatment and Research
- ATP Synthase and ATPases Research
- Radiomics and Machine Learning in Medical Imaging
- Optical Imaging and Spectroscopy Techniques
- Amino Acid Enzymes and Metabolism
- Protease and Inhibitor Mechanisms
- Enzyme function and inhibition
- Pancreatic function and diabetes
- Fermentation and Sensory Analysis
- Cell Adhesion Molecules Research
- Lipid Membrane Structure and Behavior
- Neurogenesis and neuroplasticity mechanisms
Auburn University
2011-2023
Cognitive deficits are commonly reported by patients following treatment with chemotherapeutic agents. Anthracycline-containing chemotherapy regimens associated cognitive impairment and reductions in neuronal connectivity cancer survivors, doxorubicin (Dox) is a used anthracycline. Although it has been that Dox distribution to the central nervous system (CNS) limited, considerable concentrations observed brain co-administration of certain medications. Additionally, pro-inflammatory...
The M-type phospholipase A2 receptor (PLA2R1) is a member of the C-type lectin superfamily and can internalize secreted (sPLA2) via endocytosis in non-cancer cells. sPLA2 itself was recently shown to be overexpressed prostate tumors possible mediator metastasis; however, little known about expression PLA2R1 or its function cancers. Thus, we examined primary cells (PCS-440-010) human cancer (LNCaP, DU-145, PC-3), determined effect knockdown on cytotoxicity induced by free...
Abstract Recent studies suggest that glypican-1 (GPC-1) is a biomarker for prostate cancer, but there are few elucidating the role of GPC-1 in cancer progression. We observed high expression more aggressive cell lines such as PC-3 and DU-145. While inhibition cells decreased growth migration vitro , it surprisingly increased proliferation DU-145 cells, suggesting type-dependent. Further, tumor size NCr nude mice xenografts. hypothesized discrepancy between vivo data mediated by stromal...
Doxorubicin (Dox) is a chemotherapeutic agent used widely to treat variety of malignant cancers. However, Dox chemotherapy associated with several adverse effects, including "chemobrain," the observation that cancer patients exhibit through learning and memory difficulties extending even beyond treatment. This study investigated effect treatment on as well hippocampal synaptic plasticity. Dox-treated mice (5 mg/kg weekly x 5) demonstrated impaired performance in Y-maze spatial task...
In this manuscript we report the establishment and characterization of a three-dimensional in vitro, coculture engineered prostate cancer tissue (EPCaT) disease model based upon informed by our vivo (PCa) xenograft tumor stiffness. cancer, stiffness is known to impact changes gene protein expression, alter therapeutic response, be positively correlated with an aggressive clinical presentation. To inform appropriate range for vitro model, PC-3 xenografts were established. Tissue ranged from...
Pre-clinical monitoring of tumor growth and identification distal metastasis requires a balance between accuracy expediency. Bioluminescence imaging (BLI) is often used to track but primarily limited planar 2-dimensional (2D) imaging. Consistent subject placement within standard top-mounted, single-detector small animal imager vital reducing variability in repeated same-animal measures over time. Here, we describe method for tracking development using multi-angle BLI photo-acoustic workflow....
Exploiting differences in tumor pathophysiology can enhance the delivery and antitumor activity of chemotherapeutics encapsulated drug carriers. Long‐circulating nanoparticulate carriers, such as pegylated‐sterically‐stabilized liposomes (SSL), stably entrap drug, alter disposition, improve minimize toxicity. However, control their drug‐release kinetics has limited clinical potential. Secretory phospholipases A2 (sPLA2) are over expressed excreted a variety tumors, e.g., up to 22‐fold...
Abstract Throughout the tumorigenic process, locational heterogeneities in tumor tissue microarchitecture develop as a result of aberrant angiogenesis and subsequently induced oxygen nutrient gradient within three-dimensional (3D) mass. This phenomenon often results differential stiffness between necrotic, quiescent, proliferative regions. In vitro, strong correlations have been found to exist cell culture platform acquired chemoresistance varied drug response. Therefore, accurately...
Abstract Exploiting differences in tumor pathophysiology can enhance the delivery and antitumor activity of chemotherapeutics encapsulated drug carriers. Long-circulating nanoparticle carriers, such as pegylated-sterically-stabilized liposomes (SSL), stably entrap drug, alter disposition, improve minimize toxicity. However, control their drug-release kinetics has limited clinical potential. Secretory phospholipases A2 (sPLA2) are excreted over expressed a variety tumors, e.g., up to 22-fold...
Chemotherapy-induced memory loss ("chemobrain") can occur following treatment with the widely used chemotherapeutic agent doxorubicin (DOX). However, mechanisms through which DOX induces cognitive dysfunction are not clear, and there no commercially available therapies for its or prevention. Therefore, aim of this study was to determine therapeutic potential phenyl-2-aminoethyl selenide (PAESe), an antioxidant drug previously demonstrated reduce cardiotoxicity associated treatment, against...
Abstract Glypican-1 (GPC-1) is a cell surface heparan sulfate proteoglycan that mediates proliferation, differentiation and migration. It also possess oncogenic functions in breast, pancreatic lung cancer cells, proposed biomarker for prostate cancer. Despite these data the function of GPC-1 not fully known. We addressed this gap-in-knowledge using vitro vivo mouse models. first showed was overexpressed bone metastatic cells (PC-3) but detected lymph node (LNCaP) non-cancerous (RWPE-1)....
Liposomes are spherical vesicles with a membrane composed of at least one phospholipid bilayer. They have been successfully applied for intracellular drug or genetic material delivery in many biomedical areas. In agriculture, however, their use to produce transgenic farm animals through exogenous DNA transfection spermatozoa remains unsatisfactory. Here, we tested newly designed liposome preparation effective and harmless interactions boar spermatozoa. Extended fresh semen samples were...
Abstract Alternative treatment schedules, such as low-dose, high-frequency (metronomic; METRO) of some chemotherapeutics enhance efficacy and reduce toxicity in cancers when compared to chemotherapy given conventionally (CONV). Previously, we demonstrated that prolonged exposure topotecan (30-day infusion 0.1 mg/kg/day via Alzet pump) improved survival reduced tumor volume ~120%, CONV (4 mg/kg, every 4 days) xenograft model aggressive prostate cancer athymic mice. Yet, detailed examination...
Exploiting differences in tumor pathophysiology can enhance the delivery and antitumor activity of chemotherapeutics encapsulated drug carriers. Long-circulating nanoparticle carriers, such as pegylated-sterically-stabilized liposomes (SSL), stably entrap drug, alter disposition, improve minimize toxicity. However, control their drug-release kinetics has limited clinical potential. Secretory phospholipases A2 (sPLA2) are excreted over expressed a variety tumors, e.g., up to 22-fold prostate....
Alternative treatment schedules, such as low-dose, high-frequency (metronomic; METRO) of some chemotherapeutics enhance efficacy and reduce toxicity in cancers when compared to chemotherapy given conventionally (CONV). Previously, we demonstrated that prolonged exposure topotecan (30-day infusion 0.1 mg/kg/day via Alzet pump) improved survival reduced tumor volume ~120%, CONV (4 mg/kg, every 4 days) xenograft model aggressive prostate cancer athymic mice. Yet, detailed examination the...
Abstract Background: Despite the growing array of assisted reproductive techniques, there is still a lack rapid, non-cytotoxic, and minimally invasive in situ approaches for further enhancements through cell targeting. Here we synthesized clinically relevant liposome nanoparticles real-time cellular targeting drug (doxorubicin) delivery, using pigs mares as animal models. In Experiment 1, fluorescently labeled doxorubicin-loaded (without fluorescent probe) liposomes were injected cultured...