A5100319677- Phytochemical compounds biological activities
- Glycosylation and Glycoproteins Research
- Natural product bioactivities and synthesis
- Traditional and Medicinal Uses of Annonaceae
- vaccines and immunoinformatics approaches
- Marine Sponges and Natural Products
- Angiogenesis and VEGF in Cancer
- HIV Research and Treatment
- Phytochemistry and Bioactivity Studies
- High-pressure geophysics and materials
- Monoclonal and Polyclonal Antibodies Research
- SARS-CoV-2 and COVID-19 Research
- Distributed and Parallel Computing Systems
- Cell Adhesion Molecules Research
- RNA and protein synthesis mechanisms
- Immunotherapy and Immune Responses
- Analytical chemistry methods development
- Viral gastroenteritis research and epidemiology
- Radioactive element chemistry and processing
- Biological Activity of Diterpenoids and Biflavonoids
- Viral Infectious Diseases and Gene Expression in Insects
- Carbohydrate Chemistry and Synthesis
- Analytical Chemistry and Chromatography
- 14-3-3 protein interactions
- Fungal and yeast genetics research
Qingdao University of Science and Technology
2025
Qingdao University of Technology
2025
Shanghai University of Traditional Chinese Medicine
2025
Shuguang Hospital
2025
Scripps Research Institute
2019-2024
Heilongjiang University of Science and Technology
2023-2024
Tianjin Medical University General Hospital
2024
Chinese Academy of Sciences
2006-2023
Guangzhou Institute of Geochemistry
2020-2023
International AIDS Vaccine Initiative
2019-2020
Vaccine efforts to combat the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which is responsible for current disease 2019 (COVID-19) pandemic, are focused on SARS-CoV-2 spike glycoprotein, primary target neutralizing antibodies. We performed cryo-election microscopy and site-specific glycan analysis of one leading subunit vaccine candidates from Novavax, based a full-length protein formulated in polysorbate 80 detergent. Our studies reveal stable prefusion conformation...
Aspergillus oryzae , an important filamentous fungus used in food fermentation and the enzyme industry, has been shown through genome sequencing various other tools to have prominent features its genomic composition. However, functional complexity of A. transcriptome not yet fully elucidated. Here, we applied direct high-throughput paired-end RNA-sequencing (RNA-Seq) under four different culture conditions. With high resolution sensitivity afforded by RNA-Seq, were able identify a...
Enveloped viruses hijack not only the host translation processes, but also its glycosylation machinery, and to a variable extent cover viral surface proteins with tolerogenic host-like structures. SARS-CoV-2 protein S presents as trimer on is covered by dense shield of N-linked glycans, few O-glycosites have been reported. The location O-glycans controlled large family initiating enzymes expression in cells tissues hence difficult predict. Here, we used our well-established O-glycoproteomic...
Fork head box M1 (FoxM1) is a proliferation-associated transcription factor essential for cell cycle progression. Numerous studies have documented that FoxM1 has multiple functions in tumorigenesis and its elevated levels are frequently associated with cancer The present study was conducted to investigate the expression of prognostic significance clear renal carcinoma (ccRCC). Meanwhile, function human ccRCC further investigated culture models.Real-time quantitative PCR, western blot...
The dense array of N-linked glycans on the HIV-1 envelope glycoprotein (Env), known as "glycan shield," is a key determinant immunogenicity, yet intrinsic heterogeneity confounds typical structure-function analysis. Here, we present an integrated approach single-particle electron cryomicroscopy (cryo-EM), computational modeling, and site-specific mass spectrometry (MS) to probe glycan shield structure behavior at multiple levels. We found that dynamics lead extensive network interglycan...
Four novel tetranortriterpenoids, xylogranatins A−D (1−4), with an unusual 9,10-seco skeleton were isolated from the seeds of a Chinese marine mangrove Xylocarpus granatum. Their structures determined by spectroscopic and chemical means. Xylogranatin A (1) featured unique 1,9-oxygen bridge was confirmed single-crystal X-ray diffraction, xylogranatin D (4) unprecedented C-30−C-9 linkage postulated biogenetically 3 via α-hydroxyl ketone rearrangement chemically mimicked.
Five new limonoids, granaxylocarpins A−E (1−5), were isolated from the seeds of Chinese marine mangrove Xylocarpus granatum. Granaxylocarpins A (1) and B (2) are mexicanolide-type limonoids with a 9,10-seco skeleton, granaxylocarpin C (3) possesses an 8α,30α-epoxy ring rare 1,29-oxygen bridge. The structures these characterized on basis extensive spectroscopic methods single-crystal X-ray diffraction analysis performed 4. structure xyloccensin U (4a) was revised as 4b by comparison D (4)....
The global incidence of metabolic dysfunction-associated fatty liver disease (MAFLD) is increasing annually, which has become a major public-health concern. MAFLD typically associated with obesity, hyperlipemia, or syndrome. Dietary induction one the most common methods for preparing animal models MAFLD. However, there are phenotypic differences between methionine-choline-deficient diet (MCDD) and high fat (HFD) models. To explore in hepatic acid metabolism MCDD HFD induced MAFLD, we...
Two novel tetranortriterpenoids, turrapubesins A (1) and B (2), representing the first examples of halogenated maleimide-bearing limonoids, were isolated from twigs leaves Turraea pubescens. The structures 1 2 elucidated by extensive spectroscopic analysis. Their absolute configurations determined X-ray crystallography CD analysis a dihydrogenated derivative 2. Turrapubesin exhibited weak cytotoxicity against P-388 tumor cell line.
Abstract Broadly neutralizing antibodies (bnAbs) have promise to protect against HIV infection, but induction of bnAbs by immunization is an unsolved vaccine design challenge. Germline-targeting priming immunogens aim initiate the specifically activating rare bnAb-precursor B cells that can subsequently be matured using suitable heterologous boosting and shepherding immunogens. Several pre-clinical studies, IAVI G001 human clinical trial, demonstrated ability a germline-targeting immunogen,...
Abstract Amide herbicides (AHs) were disturbed urease (UA) activity and soil microbial community, caused nutrient changes. Activity of UA was inhibited by AHs via groups chlorine, benzene ring, peptide bond (-N-/-CO-). Difference surface charge distribution mainly derived from position to connected -Cl, distance -O- ether group -N bond, difference structure/length for hydrocarbon chain, different regions negative enrichment. Developmental toxicity (DT) alachlor strongest related smaller...
Walsucochins A (1) and B (2) with an unprecedented skeleton were isolated from Walsura cochinchinensis. Their structures including absolute configuration elucidated by spectral methods. biosynthetic pathway of 1 2 was postulated. Both exhibited significant cell protecting activities against H2O2-induced PC12 damage.
A novel surface ion-imprinted polymer based on graphene oxide was synthesized for the selective adsorption of U(<sc>vi</sc>).
Abstract Vaccine efforts against the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) responsible for current COVID-19 pandemic are focused on SARS-CoV-2 spike glycoprotein, primary target neutralizing antibodies. Here, we performed cryo-EM and site-specific glycan analysis of one leading subunit vaccine candidates from Novavax based a full-length protein formulated in polysorbate 80 (PS 80) detergent. Our studies reveal stable prefusion conformation immunogen with slight...
Viruses can evade the host immune system by displaying numerous glycans on their surface "spike-proteins" that cover epitopes. We have developed an ultrasensitive "single-pot" method to assess glycan occupancy and extent of processing from high-mannose complex forms at each N-glycosylation site. Though aimed characterizing glycosylation viral spike-proteins as potential vaccines, this is applicable for analysis site-specific any glycoprotein.
Five new mexicanolide-type tetranortriterpenoids, tigloylseneganolide A (1), 2'R-methylbutanoylproceranolide (2), 2'S-methylbutanoylproceranolide (3), 2'R-cipadesin (4), and (6), as well the known 2'S-epimers of 4 6 (5 7), together with six other limonoids, were isolated from seeds Cipadessa baccifera. The structures these compounds elucidated on basis spectroscopic analyses chemical methods. 1H NMR-based conformational analysis was applied to establish absolute configuration sterically...
Abstract Three new triterpenoids, turrapubesols A–C ( 1 – 3 ), and one sesquiterpenoid, α ,4 ‐dihydroxyeudesman‐11‐ene 4 along with 12 known terpenoids were isolated from Turraea pubescens. The structures of the established on basis extensive spectroscopic analyses by a chemical transformation. previous assignment NMR data for guaidiol 5 ) was corrected. Some triterpenoids exhibited cytotoxicity against P‐388 cell line.
Two novel rearranged oleanane-type triterpenes, dysoxyhainanin A (1) possessing a unique 1,3-cyclo-2,3-seco ring with formamido-containing appendage and B (2) featuring an unprecedented 1,2-dinor-3,10:9,10-bisseco skeleton, were isolated from Dysoxylum hainanense. Dysoxyhainanin exhibited significant antibacterial activity against Gram-positive bacteria.