A5103261606- Mesenchymal stem cell research
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Transplantation: Methods and Outcomes
- Asthma and respiratory diseases
- Physiological and biochemical adaptations
- Neonatal Respiratory Health Research
- Virus-based gene therapy research
- Tissue Engineering and Regenerative Medicine
- MicroRNA in disease regulation
- Immunotherapy and Immune Responses
- Chemokine receptors and signaling
- Cancer, Hypoxia, and Metabolism
- Cancer Cells and Metastasis
- Immune Cell Function and Interaction
- Pediatric health and respiratory diseases
- Immune Response and Inflammation
- Spaceflight effects on biology
- Hematopoietic Stem Cell Transplantation
- Renal Transplantation Outcomes and Treatments
- Histone Deacetylase Inhibitors Research
- Tardigrade Biology and Ecology
- Veterinary Practice and Education Studies
- PI3K/AKT/mTOR signaling in cancer
- Nanoplatforms for cancer theranostics
- Selenium in Biological Systems
University of Yamanashi
2021-2022
Tohoku University
2011-2021
Tohoku University Hospital
2015-2017
Japan Science and Technology Agency
2006
Iwate University
2000
Mesenchymal stromal cells (MSCs), also called mesenchymal stem cells, migrate and function as in tumor tissues. The effects of MSCs on growth are controversial. In this study, we showed that increase proliferation vitro promote vivo. We further analyzed the mechanisms underlie these effects. For use vivo experiments, established a bone marrow-derived cell line from isolated C57BL/6 mice. Effects murine were coculture model with B16-LacZ cells. Both co-culture treatment MSC-conditioned media...
Multipotent stromal cells (MSCs) ameliorate several types of lung injury. The differentiation MSCs into specific at the injury site has been considered as important process in MSC effect. However, although reduce destruction an elastase-induced emphysema model, is relatively rare, suggesting that does not adequately explain recuperation observed. Humoral factors secreted by may also play role ameliorating emphysema. To confirm this hypothesis, was induced lungs C57BL/6 mice intratracheal...
MSCs are nonhematopoietic stem cells capable of differentiating into various mesoderm-type cells. have been considered to be a potential vehicle for cell-based gene therapy because relatively easily expanded in vitro and the propensity migrate proliferate tumor tissue after systemic administration. Here, we demonstrated tropism mouse multiple tissues lung i.v. injection green fluorescent protein-positive vivo. We transduced CX3CL1 (fractalkine), an immunostimulatory chemokine, ex vivo using...
Current hypotheses suggest that aberrant wound healing has a critical role in the pathogenesis of idiopathic pulmonary fibrosis (IPF). In these hypotheses, continuous TGF-β1 secretion by alveolar epithelial cells (AECs) abnormal promoting fibroblast differentiation into myofibroblasts. Mesenchymal stem (MSCs) home to injury site and reduce secreting multifunctional antifibrotic humoral factors IPF. this study, we show MSCs can correct inadequate-communication between mesenchymal through STC1...
Previous studies have demonstrated that mesenchymal stromal cells (MSCs) enhance cell survival through upregulation and secretion of stanniocalcin-1 (STC1). This study shows MSC-derived STC1 promotes lung cancer by uncoupling oxidative phosphorylation, reducing intracellular reactive oxygen species (ROS), shifting metabolism towards a more glycolytic metabolic profile. upregulated protein 2 (UCP2) in injured A549 an STC1-dependent manner. Knockdown UCP2 reduced the ability MSCs recombinant...
Glioblastoma is the most malignant human brain tumor and has a dismal prognosis; however, some patients show long-term survival. The interaction between costimulatory molecule OX40 its ligand OX40L generates key signals for T-cell activation. augmentation of this enhances antitumor immunity. In present study, we explored whether signaling responsible adaptive immunity against glioblastoma also established therapeutic antiglioma vaccination therapy. Tumor specimens were obtained from with...
The lung is one of the organs to which cancers from solid tumors frequently metastasize. Multiple in are usually treated by systemic chemotherapy because lack efficient methods targeting antitumor agents lung. Although intratracheal administration an ideal route for multiple tumors, often harmful organ or induce inflammation. Mesenchymal stem cells (MSCs), nonhematopoietic capable differentiating into various mesoderm-type cells, have a propensity migrate and proliferate tumor tissues after...
The bone marrow microenvironment comprises multiple cell niches derived from mesenchymal stem cells. However, the molecular mechanism of differentiation is poorly understood. transcription factor GATA2 indispensable for hematopoietic function as well other lineages, suggesting that it may maintain cells in an immature state and also contribute to their differentiation. To explore this possibility, we established conditional knockout mice. Differentiation GATA2-deficient into adipocytes...
Mesenchymal stromal cells (MSCs) are multipotent progenitor and there is much interest in how MSCs contribute to the regulation of tumor microenvironment. Whether exert a supportive or suppressive effect on progression still controversial, but likely dependent variety factors that tumor-type dependent. Multiple myeloma (MM) characterized by growth malignant plasma bone marrow. It has been shown MM governed MSCs, which act as stroma cells. Although created via mutual communication between...
Fractalkine (CX3CL1) is a unique membrane-bound CX3C chemokine that serves as potent chemoattractant for lymphocytes. The hypothesis of this study dendritic cells (DC) genetically modified ex vivo to overexpress fractalkine would enhance the T cell-mediated cellular immune response with consequent induction anti-tumor immunity suppress tumor growth. To prove hypothesis, established tumors different mouse cancer (B16-F10 melanoma, H-2b, and Colon-26 colon adenocarcinoma, H-2d) were treated...
Rationale: OX40–OX40 ligand (OX40L) interactions have been proposed to support induction of allergic airway inflammation, which may be attributable OX40 signaling in CD4+ helper T cells for adaptive immune responses. However, a possible involvement natural killer (NKT) the pathogenesis suggests that underlying mechanisms are not yet fully elucidated.Objectives: We aimed characterize OX40-modulated cellular contribution inflammation mouse model house dust mite (HDM) allergen exposure.Methods:...
Marrow stromal cells (MSCs) isolated from mesenchymal tissues can propagate in vitro to some extent and differentiate into various tissue lineages be used for cell-based therapies. Cellular senescence, which occurs readily continual MSC culture, leads loss of these characteristic properties, representing one the major limitations achieving potential MSCs. In this study, we investigated effect lysophosphatidic acid (LPA), a ubiquitous metabolite membrane phospholipid synthesis, on senescence...
Mycobacteria are among the most common causes of hypersensitivity pneumonitis (HP), but controversy persists with regard to involvement infectious potency organism in mycobacterial HP (hot tub lung). This study aimed establish a mouse model hot lung clarify its pathophysiology. Mice were exposed intranasally formalin-killed Mycobacterium avium from patient (HP strain) or chronic pulmonary infection (non-HP strain), and bronchoalveolar lavage fluids tissues evaluated for allergic...
Interstitial lung disease (ILD) is reported as a serious adverse event in cancer patients treated with gefitinib, an epidermal growth factor receptor tyrosine kinase inhibitor (EGFR‐TKI). However, the mechanisms of ILD associated gefitinib remain unknown. To address molecular ILD‐associated we determined effect treatment on surfactant protein expression vitro and vivo . Gefitinib suppressed (SP)‐A H441 human adenocarcinoma cells expressing SP‐A, ‐B, ‐C ‐D by inhibiting signal. Next, (200...
Live animals are used in surgical skills training wet lab, which has undeniable effectiveness for the development of future surgeons.However, where such is provided, animal welfare a major consideration.Increasingly, institutions that offer wet-lab incorporating ethics and welfare-related content into their courses, but education yet to be evaluated quantitatively.We investigated whether course affected trainees by measuring increase ethical awareness using visual analog scale questionnaires...
As shown in our previous studies, the intratracheal-administration of STC1 (stanniocalcin-1) ameliorates pulmonary fibrosis by reducing oxidative and endoplasmic reticulum stress through uncoupling respiration a bleomycin-treated mouse model. However, overall effect on metabolism was not examined. Therefore, we first conducted comprehensive metabolomics analysis to screen metabolic changes induced an alveolar epithelial cell line using capillary electrophoresis time-of-flight mass...
Airway surface fluids are mainly secreted from submucosal glands, and play important roles in the defense of airways via up-regulation mucociliary transport, resulting an exclusion many microbes or foreign substances. Although there articles concerning importance Toll-like receptors (TLRs) airway immune systems, whether TLRs directly cooperate with tracheal glands to increase secretion remains unknown. We investigated effects ligands three TLR subtypes (TLR2, TLR3, TLR4) on physiologic...
The functional interplay between cancer cells and marrow stromal (MSCs) has attracted a great deal of interest due to the MSC tropism for tumors but remains be fully elucidated. In this study, we investigated human MSC-secreted paracrine factors that appear have critical functions in stem cell subpopulations. We show MSC-conditioned medium reduced cell-enriched subpopulation, which was detected as side population quiescent (G0) cycle fraction lung by virtue fibroblast growth factor 10...
Abstract Lung cancer is the most common cause of cancer‐related death in developed countries; therefore, generation effective targeted therapeutic regimens essential. Recently, gene therapy approaches toward malignant cells have emerged as attractive molecular therapeutics. Previous studies indicated that stanniocalcin‐1 (STC‐1), a hormone involved calcium and phosphate homeostasis, positively regulates proliferation, apoptosis resistance, glucose metabolism lung cell lines. In this study,...
Airway serous secretion is essential for the maintenance of mucociliary transport in airway mucosa, which responsible upregulation mucosal immunity. Although there are many articles concerning importance Toll-like receptors (TLRs) immune systems, direct relationship between TLRs and has not been well investigated. Here, we focused on whether TLR5 ligand flagellin, one components Pseudomonas aeruginosa, involved secretion. Freshly isolated swine tracheal submucosal gland cells were prepared,...
Mast cells (MCs) play crucial roles in innate immunity to parasitic and bacterial infections as well hypersensitivity, such the induction exacerbation of allergy autoimmune diseases. The regulatory mechanisms for MC development effector functions are great interest developing novel therapeutic strategies against disorders. Here we report establishment novel, immortalized lines from bone marrow (BM) a temperature-sensitive mutant SV40 large T antigen-transgenic mice (termed SVMCs). BM...