A5038606401- Mast cells and histamine
- Asthma and respiratory diseases
- Monoclonal and Polyclonal Antibodies Research
- Contact Dermatitis and Allergies
- Heat Transfer and Optimization
- Heat Transfer and Boiling Studies
- Drug-Induced Adverse Reactions
- Virology and Viral Diseases
- Virus-based gene therapy research
- Urological Disorders and Treatments
- Food Allergy and Anaphylaxis Research
- Refrigeration and Air Conditioning Technologies
- Receptor Mechanisms and Signaling
- Immune Cell Function and Interaction
- Polyamine Metabolism and Applications
- Nitric Oxide and Endothelin Effects
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Urticaria and Related Conditions
- Ureteral procedures and complications
- Viral Infections and Outbreaks Research
- Allergic Rhinitis and Sensitization
- RNA Research and Splicing
- Chemical Synthesis and Analysis
- Immunotoxicology and immune responses
- Fluid Dynamics and Mixing
International AIDS Vaccine Initiative
2011-2025
Holy Cross Hospital
2024
University of Miami
2024
Cornell University
1974-2018
Western Michigan University
1996-2017
Human Immunome Project
2003-2009
National Institute of Allergy and Infectious Diseases
2004-2007
National Institutes of Health
2004-2007
Pearl River Community College
1999-2007
Institute of Pharmacology
2004
This paper reports the development of an experimentally validated model for pressure drop during intermittent flow condensing refrigerant R134a in horizontal microchannels. Two-phase drops were measured five circular channels ranging hydraulic diameter from 0.5 mm to 4.91 mm. For each tube under consideration, measurements first taken over entire range qualities 100% vapor liquid. In addition, tests conducted different mass fluxes between 150 kg/m2-s and 750 kg/m2-s. Results previous work by...
This paper reports the development of an experimentally validated model for pressure drop during intermittent flow condensing refrigerant R134a in horizontal, noncircular microchannels. Two-phase drops were measured six channels ranging hydraulic diameter from 0.42 mm to 0.84 mm. The tube shapes included square, rectangular, triangular, barrel-shaped, and others. For each under consideration, measurements taken over entire range qualities vapor liquid at five different mass fluxes between...
Abstract We examined whether three cytokines that promote mouse mast cell development, the c-kit ligand stem factor (SCF), IL-3, or IL-4, also can directly stimulate modulate peritoneal (PMC) mediator release. Challenge of purified PMC with rat rSCF164 at 20 to 100 ng/ml for 30 min induced a modest release serotonin (5-HT), whereas IL-3 IL-4 did not 5-HT Experiments in which were exposed each cytokine 15 min, and then DNP-HSA Ag anti-IgE antibody further showed SCF, but had an additive...
A number of studies have demonstrated that the retroviral protein integrase (IN) alone is sufficient to carry out two discrete steps required for integration: endonucleolytic processing viral DNA ends and cleavage joining host processed termini. Little known about biochemical biophysical mechanisms involved in these reactions. Here, we employ vitro assays Rous sarcoma virus IN demonstrate first time capable multiple turnover both The calculated reaction 0.26 cleavages/min/mol IN. Our steady...
Abstract The neurotransmitter serotonin (5-hydroxytryptamine (5-HT)) is implicated in enhancing inflammatory reactions of skin, lung, and gastrointestinal tract. To determine whether 5-HT acts, part, through mast cells (MC), we first established that mouse bone marrow-derived MC (mBMMC) human CD34+-derived (huMC) expressed mRNA for multiple receptors. We next determined the effect on degranulation, adhesion, chemotaxis. found no evidence degranulates or modulates IgE-dependent activation....
In immunity, reactive oxygen species (ROS) and nitric oxide (NO) are important antimicrobial agents regulators of cell signaling activation pathways. However, the cellular sources ROS NO much debated. Particularly, there is contention over whether mast cells, key secretory cells in allergy can generate these chemical species, if so, they functional significance. We therefore examined directly by flow cytometry capacity to intracellular using respective cell-permeable fluorescent probes...
Abstract Mast cells and macrophages live in close proximity vivo reciprocally regulate one another’s function various ways. Although activated possess a powerful reactive oxygen species (ROS) generating system, there is conflicting evidence regarding whether mast can produce ROS. We used the highly sensitive real-time chemiluminescent probe Pholasin to examine ROS release by peritoneal isolated from OVA-sensitized rats. Macrophages stimulated with PMA (0.8 μM) or ionomycin (1 μM), but not...
ABSTRACT Recombinant vesicular stomatitis virus (rVSV) has shown great potential as a new viral vector for vaccination. However, the prototypic rVSV described previously was found to be insufficiently attenuated clinical evaluation when assessed neurovirulence in nonhuman primates. Here, we describe attenuation, neurovirulence, and immunogenicity of vectors expressing human immunodeficiency type 1 Gag. These were by combinations following manipulations: N gene translocations (N4), G...
The surface specimen technique was used to study both spiral organs of 28 normal guinea pigs four age groups: <24 hours, 6 weeks, 3 months and 1 year. Damaged hair cells were recorded for the whole each organ on cochleograms. mean percentage number outer damaged per group found increase as a power function age. In animals aged hours 0.45%; in 6-week animals, 1.85%; 3-month 3.19%; 1-year 6.82%. At all ages cell loss maximal third row, towards apex cochlea. Inner very slight, with maximum 9 inner
DNA priming has previously been shown to elicit augmented immune responses when administered by electroporation (EP) or codelivered with a plasmid encoding interleukin-12 (pIL-12). We hypothesized that the efficacy of prime and recombinant adenovirus 5 boost vaccination regimen (DNA/rAd5) would be improved incorporating these strategies into phase, as determined pathogenic simian immunodeficiency virus SIVmac239 challenge outcome. The whole proteome was delivered in separate plasmids...
SummaryBackgroundLassa fever (LF) is a zoonotic haemorrhagic disease caused by Lassa virus (LASV), which endemic in West African countries. The multimammate rat the main animal reservoir and its geographic range expected to expand due influences like climate change land usage, this will place larger parts of Africa at risk. We conducted preclinical development on promising experimental vaccine that allowed advancement into human trials.MethodsThe LF based vesicular stomatitis (VSV) vector...
Abstract IFN-gamma regulates various aspects of rodent peritoneal mast cell function, including mediator release, growth, TNF-alpha-mediated cytotoxicity, and MHC class II expression. We investigated whether the suppressive action on IgE/Ag-mediated degranulation cells is mediated via synthesis nitric oxide. Incubation mouse with L-NMMA, an inhibitor oxide synthase, or in medium lacking precursor L-arginine reversed inhibitory effect Ag-induced serotonin release. Furthermore, donors sodium...
Abstract An experimental investigation of two-phase flow mechanisms during condensation refrigerant R134a in small diameter round and rectangular tubes was conducted. A 4.91 mm tube, four with hydraulic diameters ranging from 1 – 4 were studied to characterize the influence tube miniaturization on mechanisms. For each under consideration, recorded over entire range qualities 0 &lt; x 1, for five different mass fluxes between 150 kg/m2-s 750 kg/m2-s. Approximately 50 data points obtain a...
Abstract Brown Norway (BN) rats given mercuric chloride (HgCl 2 ), gold (Au) salts or D‐penicillamine develop a T helper (Th2) cell‐mediated autoimmune syndrome. The recent observation of tissue injury within 24 h HgCl treatment suggested the involvement non‐T cell. We therefore examined effect these compounds on rat mast cells in vitro . Incubation BN peritoneal with enhanced release serotonin response to IgE cross‐linking agents. Mast from Lewis rats, strain not susceptible syndrome vivo ,...