A5077504869- Monoclonal and Polyclonal Antibodies Research
- HIV Research and Treatment
- Immune Cell Function and Interaction
- Glycosylation and Glycoproteins Research
- SARS-CoV-2 and COVID-19 Research
- Cytomegalovirus and herpesvirus research
- Influenza Virus Research Studies
- Viral gastroenteritis research and epidemiology
- Immune responses and vaccinations
- Galectins and Cancer Biology
- Viral Infections and Vectors
- Immunodeficiency and Autoimmune Disorders
- COVID-19 Clinical Research Studies
- Escherichia coli research studies
- HIV/AIDS Research and Interventions
- Viral Infections and Outbreaks Research
- Immunotherapy and Immune Responses
- Respiratory viral infections research
- Protein purification and stability
- Adenosine and Purinergic Signaling
- Herpesvirus Infections and Treatments
- Poxvirus research and outbreaks
- Reproductive System and Pregnancy
- Vibrio bacteria research studies
- Inflammasome and immune disorders
Ragon Institute of MGH, MIT and Harvard
2014-2025
Moderna Therapeutics (United States)
2022-2024
Massachusetts General Hospital
2024
Harvard University
2014-2023
Massachusetts Institute of Technology
2023
Health and Human Development (2HD) Research Network
2022
Mpox virus (MPXV) caused a global outbreak in 2022. Although smallpox vaccines were rapidly deployed to curb spread and disease among those at highest risk, breakthrough was noted after complete immunization. Given the threat of additional zoonotic events virus's evolving ability drive human-to-human transmission, there is an urgent need for MPXV-specific vaccine that confers protection against MPXV strains related orthopoxviruses. Here, we demonstrate mRNA-lipid nanoparticle encoding set...
Intradermal Bacillus Calmette-Guérin (BCG) is the most widely administered vaccine, but it does not sufficiently protect adults against pulmonary tuberculosis. Recent studies in nonhuman primates show that intravenous BCG administration offers superior protection Mycobacterium tuberculosis ( Mtb ). We used single-cell analysis of bronchoalveolar lavage cells from rhesus macaques vaccinated via different routes and doses to identify alterations immune ecosystem airway following vaccination....
Group 2B β-coronaviruses (sarbecoviruses) have caused regional and global epidemics in modern history. Here, we evaluate the mechanisms of cross-sarbecovirus protective immunity, currently less clear yet important for pan-sarbecovirus vaccine development, using a panel alphavirus-vectored vaccines covering bat to human strains. While vaccination does not prevent virus replication, it protects against lethal heterologous disease outcomes both severe acute respiratory syndrome coronavirus 2...
Shigella infection is the second leading cause of death due to diarrheal disease in young children worldwide. With rise antibiotic resistance, initiatives design and deploy a safe effective vaccine are urgently needed. However, efforts date have been hindered by limited understanding immunological correlates protection against shigellosis. We applied systems serology perform comprehensive analysis Shigella-specific antibody responses sera obtained from volunteers before after experimental...
SummaryBackgroundLassa fever (LF) is a zoonotic haemorrhagic disease caused by Lassa virus (LASV), which endemic in West African countries. The multimammate rat the main animal reservoir and its geographic range expected to expand due influences like climate change land usage, this will place larger parts of Africa at risk. We conducted preclinical development on promising experimental vaccine that allowed advancement into human trials.MethodsThe LF based vesicular stomatitis (VSV) vector...
The 2013 Ebola epidemic in Central and West Africa heralded the emergence of wide-spread, highly pathogenic viruses. successful recombinant vector vaccine against (rVSVΔG-ZEBOV-GP) will limit future outbreaks, but identifying mechanisms protection is essential to protect most vulnerable. Vaccine-induced antibodies are key determinants efficacy, yet mechanism by which vaccine-induced prevent infection remains elusive. Here, we exploit a break long-term efficacy non-human primates identify...
Beyond the acute illness caused by severe respiratory coronavirus 2 (SARS-CoV-2) infection, about one-fifth of infections result in long-term persistence symptoms despite apparent clearance infection. Insights into mechanisms that underlie postacute sequelae COVID-19 (PASC) will be critical for prevention and clinical management complications COVID-19. Several hypotheses have been proposed may account development PASC, including virus dysregulation immune responses. Among immunological...
The goal of this study was to investigate the relationship between anti-SARS-CoV-2-Spike IgG titers passively transferred fetus from maternal vaccination during pregnancy and timing infant SARS-CoV-2 infection. Pregnant, vaccinated individuals (n = 105) their infants 107) were enrolled in a prospective cohort July 2021 June 2022, linking anti-Spike titer at birth risk infection first fifteen months life. Cord blood sera collected delivery two six age. Anti-SARS-CoV-2-Spike levels quantified...
Respiratory syncytial virus (RSV) is among the most common causes of lower respiratory tract infection (LRTI) and hospitalization in infants. However, mechanisms immune control infants remain incompletely understood. Antibody profiling against attachment (G) fusion (F) proteins children less than 2 years age, with mild (outpatients) or severe (inpatients) RSV disease, indicated substantial age-dependent differences RSV-specific immunity. Maternal antibodies were detectable for first 3 months...
While Epstein-Barr virus causes mostly asymptomatic infection, associated malignancies, and autoimmune lymphoproliferative diseases occur. To dissect the evolution of humoral immune responses over course EBV infection to gain a better understanding potential contribution antibody (Ab) function viral control, we comprehensively profiled Ab specificities Fc-functionalities using systems serology VirScan. functions against latent (EBNA1), early (p47/54) two late (gp350/220 VCA-p18) proteins...
Abstract Monkeypox virus (MPXV) caused a global outbreak in 2022, fueled by behaviorally-altered and enhanced human-to-human transmission. While smallpox vaccines were rapidly deployed to curb spread disease among those at highest risk, breakthrough was noted after complete immunization. Given the imminent threat of additional zoonotic events as well virus’ evolving ability drive transmission, there is an urgent need for development MPXV-specific vaccine that able also confer broad...
Abstract The introduction of vaccines has inspired new hope in the battle against SARS-CoV-2. However, emergence viral variants, absence potent antivirals, left world struggling with uncertain nature this disease. Antibodies currently represent strongest correlate immunity COVID-19, thus we profiled earliest humoral signatures a large cohort severe and asymptomatic COVID-19 individuals. While SARS-CoV-2-specific immune response evolved rapidly survivors non-survivors exhibited blunted...
The reduced effectiveness of COVID-19 vaccines due to the emergence variants concern (VOCs) necessitated use vaccine boosters bolster protection against disease. However, it remains unclear how boosting expands protective breadth when primary platforms are distinct and containing VOC spike(s) broaden humoral responses. Here, we report that composed recombinant spike antigens ancestral (prototype) Beta VOCs elicit a robust, pan-VOC, multi-functional response in non-human primates largely...
Vibriocidal antibodies are currently the best characterised correlate of protection against cholera and used to gauge immunogenicity in vaccine trials. Although other circulating antibody responses have been associated with a decreased risk infection, correlates not comprehensively compared. We aimed analyse antibody-mediated from both V cholerae infection cholera-related diarrhoea.
Under the ever-present threat of a pandemic influenza strain, evolution broadly reactive, neutralizing, functional, humoral immune response may hold key to protection against both circulating and emerging strains. We apply systems approach profile hemagglutinin- neuraminidase-specific signatures that track with broad immunity in cohort vaccinated individuals validate these findings second longitudinal cohort. Multivariate analysis reveals presence unique pre-existing Fcγ-receptor-binding...
People with HIV (PWH) appear to be at higher risk for suboptimal pathogen responses and worse COVID-19 outcomes, but the effects of host factors on humoral repertoire remain unclear. We assessed antibody isotype/subclass Fc-receptor binding Luminex arrays non-SARS-CoV-2 SARS-CoV-2 among antiretroviral therapy-treated (ART-treated) PWH. Among entire cohort, infection was associated cytomegalovirus (CMV) (vs. COVID- cohort ), potentially signifying increased susceptibility or a consequence...
Shigellosis is the second leading cause of diarrheal death in children younger than five years age globally. At present, there no broadly licensed vaccine against shigella infection. Previous candidates have failed at providing protection for young endemic settings. Improved understanding correlates Shigella infection and severe shigellosis living settings needed. Here, we applied a functional antibody profiling approach to define Shigella-specific responses versus older individuals with...
SUMMARY Pregnancy represents a unique tolerogenic immune state which may alter susceptibility to infection and vaccine-response. Here we characterized humoral immunity seasonal influenza vaccine strains in pregnant non-pregnant women. Pregnant women had reduced hemagglutinin subtype-1 (H1)-IgG, IgG1, IgG2, hemagglutination inhibition group 1 2 stem IgG. However, H1-specific avidity FcγR1 binding increased. Influenza-antibodies pregnancy distinct Fc Fab glycans by di-galactosylation...