A5043752309- Vector-borne infectious diseases
- Antimicrobial Resistance in Staphylococcus
- Clostridium difficile and Clostridium perfringens research
- Mosquito-borne diseases and control
- Viral Infections and Vectors
- 3D Printing in Biomedical Research
- Plant Virus Research Studies
- Cancer therapeutics and mechanisms
- Leptospirosis research and findings
- Pharmacological Effects of Natural Compounds
- Viral gastroenteritis research and epidemiology
- Parasites and Host Interactions
- Antibiotic Resistance in Bacteria
- Escherichia coli research studies
- Quinazolinone synthesis and applications
- Plant Pathogenic Bacteria Studies
- Bacterial biofilms and quorum sensing
- Gut microbiota and health
- Microscopic Colitis
- Bacterial Identification and Susceptibility Testing
- Studies on Chitinases and Chitosanases
- Antibiotics Pharmacokinetics and Efficacy
- Diagnosis and treatment of tuberculosis
- Dialysis and Renal Disease Management
- Infectious Diseases and Mycology
Hamad Medical Corporation
2025
Christian Medical College & Hospital
2012-2024
Massachusetts General Hospital
2024
Christian Medical College
2012-2024
University of Notre Dame
2016-2023
Emulate (United States)
2021-2023
Tufts Medical Center
2014
Indira Gandhi Medical College
2014
Scrub typhus, a bacterial zoonosis caused by Orientia tsutsugamushi, may cause multiorgan dysfunction syndrome (MODS) and is associated with significant mortality. This study was undertaken to document the clinical laboratory manifestations complications time trends factors mortality in patients scrub typhus infection.This retrospective study, done at university teaching hospital, included 623 admitted between 2005 2010 typhus. The diagnosis established positive IgM ELISA and/or...
Conventional preclinical models often miss drug toxicities, meaning the harm these drugs pose to humans is only realized in clinical trials or when they make it market. This has caused pharmaceutical industry waste considerable time and resources developing destined fail. Organ-on-a-Chip technology potential improve success development pipelines, as can recapitulate organ-level pathophysiology responses; however, systematic quantitative evaluations of Organ-Chips' predictive value have not...
SummaryObjectivesThis study sought to document the clinical and laboratory manifestations, genetic variability, outcomes of scrub typhus, an often severe infection caused by Orientia tsutsugamushi, in South India.MethodsPatients admitted a large teaching hospital with IgM ELISA-confirmed typhus were evaluated. Clinical examination thorough search for eschar, testing, chest X-ray, outcome documented analyzed. Additionally, 410-bp region 56-kDa type-specific antigen gene O. tsutsugamushi was...
Abstract Scrub typhus, an acute febrile illness that is widespread in the Asia-Pacific region, caused by bacterium Orientia tsutsugamushi, which displays high levels of antigenic variation. We conducted investigation to identify circulating genotypes O. tsutsugamushi 3 scrub typhus–endemic geographic regions India: South India, Northern and Northeast India. Eschar samples collected during September 2010–August 2012 from patients with typhus were subjected 56-kDa type-specific PCR sequencing...
The quinazolinones are a new class of antibacterials with in vivo efficacy against methicillin-resistant Staphylococcus aureus (MRSA). target cell wall biosynthesis and have unique mechanism action by binding to the allosteric site penicillin-binding protein 2a (PBP 2a).
Scrub typhus, caused by Orientia tsutsugamushi, is endemic in the Asia-Pacific region. Mortality high if untreated, and even with treatment as 10-20%, further knowledge of immune response during scrub typhus needed. The current study was aimed at comparing plasma levels a variety inflammatory mediators patients controls South India order to map broader cytokine profile their relation disease severity clinical outcome.We examined several cytokines (n = 129) compared healthy 31) infectious...
We report herein the syntheses of 79 derivatives 4(3H)-quinazolinones and their structure–activity relationship (SAR) against methicillin-resistant Staphylococcus aureus (MRSA). Twenty one analogs were further evaluated in vitro assays. Subsequent investigation pharmacokinetic properties singled out compound 73 ((E)-3-(5-carboxy-2-fluorophenyl)-2-(4-cyanostyryl)quinazolin-4(3H)-one) for study. The synergized with piperacillin-tazobactam (TZP) both vivo a clinically relevant mouse model MRSA...
Abstract Human organ-on-a-chip (Organ-Chip) technology has the potential to disrupt preclinical drug discovery and improve success in development pipelines as it can recapitulate organ-level pathophysiology clinical responses. The Innovation Quality (IQ) consortium formed by multiple pharmaceutical biotechnology companies confront this challenge published guidelines that define criteria for qualifying models, however, systematic quantitative evaluation of predictive value Organ-Chips not yet...
ABSTRACT Background and Aims The neutrophil‐to‐lymphocyte ratio (NLR) is a cost‐effective indicator of inflammation, which may impact decisions regarding therapy for patients undergoing continuous renal replacement (CRRT), even with ongoing clinical arguments. This study aimed to examine the correlation between NLR prognosis critically ill CRRT, specifically about mortality morbidity. Additionally, sought assess NLR's potential as prognostic CRRT initiation. Methods Data were retrospectively...
ABSTRACT The oxadiazole antibacterials target the bacterial cell wall and are bactericidal. We investigated synergism of ND-421 with commonly used β-lactams non-β-lactam antibiotics by checkerboard method time-kill assays. synergizes well β-lactam antibiotics, it also exhibits a long postantibiotic effect (4.7 h). evaluated in vivo efficacy murine neutropenic thigh infection model alone combination oxacillin. has itself clinically relevant (1.49 log 10 reduction for ND-321 versus 0.36...
Clostridioides difficile is an anaerobic Gram-positive bacterium that colonizes the gut of patients treated with broad-spectrum antibiotics. The normal microflora prevents C. colonization; however, dysbiosis by treatment antibiotics causes recurrent infection (CDI) in 25% patients. There are no fully effective for multiple CDIs. We report herein oxadiazole exhibit bactericidal activity against vegetative cells. screened a library 75 oxadiazoles ATCC 43255. findings from this collection...
Clostridioides difficile infection (CDI) is the most lethal of five CDC urgent public health treats, resulting in 12,800 annual deaths United States alone [Antibiotic Resistance Threats States, 2019 (2019), www.cdc.gov/DrugResistance/Biggest-Threats.html]. The high recurrence rate and inability antibiotics to treat such infections mandate discovery new therapeutics. A major challenge with CDI production spores, leading multiple recurrences 25% patients [C. P. Kelly, J. T. LaMont, N. Engl....
A structure–activity relationship (SAR) for the oxadiazole class of antibacterials was evaluated by syntheses 72 analogs and determination minimal-inhibitory concentrations (MICs) against ESKAPE panel bacteria. Selected compounds were further in vitro toxicity, plasma protein binding, pharmacokinetics (PK), a mouse model methicillin-resistant Staphylococcus aureus (MRSA) infection. Oxadiazole 72c shows potent antibacterial activity, exhibits low clearance, high volume distribution, 41% oral...
The ribosomally produced antimicrobial peptides of bacteria (bacteriocins) represent an unexplored source membrane-active antibiotics. We designed a library linear from circular bacteriocin and show that pore-formation dynamics in bacterial membranes are tunable via selective amino acid substitution. observed antibacterial interpeptide synergy indicating fundamentally altering interactions with the membrane enables synergy. Our findings suggest approach for engineering through rational...
The aim of this study was to clarify the contentious taxonomic classification Rhinosporidium seeberi, cause human rhinosporidiosis, which may have treatment implications.PCR used amplify internal transcribed spacer (ITS)-2 region from genomic DNA aetiological agent obtained a sample rhinosporidiosis lesions. amplicon sequenced and organism identified using Basic Local Alignment Search Tools (BLAST).Phylogenetic analysis revealed that clustered along with R. seeberi isolated humans also...
Immunity protective against shigella infection targets the bacterial O-specific polysaccharide (OSP) component of lipopolysaccharide. A multivalent vaccine would ideally target most common global Shigella species and serotypes such as flexneri 2a, S. 3a, 6, sonnei. We previously reported development conjugate vaccines (SCVs) targeting 2a (SCV-Sf2a) 3a (SCV-Sf3a) using a platform squaric acid chemistry conjugation approach carrier protein rTTHc, 52 kDa recombinant fragment heavy chain tetanus...
Staphylococcus aureus (S. aureus) causes a variety of infections, ranging from mild skin infection to blood stream infections and deep seated infections. As Stapylococcus bacteremia (SAB) has the tendency cause endovascular metastatic complications can occur at almost all sites body. Hence, SAB is associated with increased morbidity mortality in spite appropriate antimicrobial treatment. The virulence S. determined by presence adhesins toxins, which behave like superantigens (SAgs) leads...
A major challenge for chemotherapy of bacterial infections is perturbation the intestinal microbiota. Clostridioides difficile a Gram-positive bacterium gut that can thrive under this circumstance. Its production dormant and antibiotic-impervious spores results in chronic disruption normal flora debilitating diarrhea infection. C. responsible 12,800 deaths per year United States. Here, we report discovery 2-(4-(3-(trifluoromethoxy)phenoxy)picolinamido)benzo[d]oxazole-5-carboxylate as an...