Background: The rapid emergence of antimicrobial resistance among Gram-positive organisms, especially staphylococci, has become a serious clinical challenge. Efflux machinery and biofilm formation are considered two the main causes therapy failure. Aim: Our study aims to evaluate antibiofilm efflux pump inhibitory activity antifungal ketoconazole against multidrug-resistant (MDR) Staphylococcus aureus. Methods: Ketoconazole was tested for its effect on following: minimum concentrations...

A PCR technique to differentiate pathogenic enteric Escherichia coli strains in a field setting was evaluated. Among 76 children with acute diarrhea, this identified 12 (16%) enterotoxigenic E. coli, 6 (8%) enteropathogenic and 1 (1%) enteroinvasive infection. Compared the conventional assays, method proved be simpler, more rapid, inexpensive therefore suitable for application developing-country setting.

Hemophagocytic lymphohistiocytosis (HLH) is a rare and life-threatening disorder of immune regulation that can rapidly progress to end-organ damage death. HLH characterised by uncontrolled activation cytotoxic T lymphocytes, natural killer cells, macrophages lead cytokine storm. Primary genetic seen predominantly in the pediatric population, resulting from inherited defects genes regulating function. Secondary HLH, on other hand, affect individuals any age triggered various conditions such...

Streptococcus pneumoniae infection is a serious problem worldwide and the case fatality rate remains high. The aim of this study was to analyze distribution pneumococcal serotypes causing invasive disease (IPD), survey potential coverage present future vaccines, investigate differences between groups with regard manifestation, rate, age, other risk factors. Isolates from 244 consecutive patients IPD were collected at Christian Medical College, Vellore, India January 2007 June 2011, clinical...

Aim: The aim was to evaluate the in vitro activity of fosfomycin against common uropathogens a fosfomycin-naive population India. Methods: authors prospectively collected and tested all consecutive isolates Escherichia coli Enterococcus spp. from urine samples between December 2009 April 2010. Results: A total 3141 were included study, 2416 E. 725 Fosfomycin most active antibiotic both pathogens with an overall susceptibility 83 99% for spp, respectively. Among coli, 47.6% extended-spectrum...

An immunoblotting system was developed for typing of oxacillin-resistant Staphylococcus aureus. Clinical isolates recovered during a 40-month period at single institution were evaluated with this scheme. Results compared susceptibility patterns and bacteriophage results 100 clinical plasmid fingerprints 14 isolates. Immunoblotting found to be useful method good reproducibility that distinguished seven major groups patient clinically epidemiologically related. Susceptibility showed specific...

BackgroundCo production of 16S rRNA methylases gene and β-Lactamase among Enterobacteriaceae isolates conferring resistance to both therapeutic options has serious implications for clinicians worldwide.MethodsTo study co existence (armA, rmtA, rmtB, rmtC, rmtD, npmA) (blaTEM-1, blaSHV-12, blaCTX-M-14) genes, we screened all phenotypic positive producing enterobacteriaceae by polymerase chain reaction (PCR) targeting above genes. A total 330 strains were collected during period out that 218...

Background: Lung cancer is one of the main human health threats. Survival lung patients depends on timely detection and diagnosis. Among genetic irregularities that control development progression, there are microRNAs (miRNAs). This study aimed to assess plasma level circulating miRNA-17 miRNA-222 as non-invasive markers in non-small-cell (NSCLC) patients. Patients methods: A total 40 with NSCLC 20 healthy controls who were matched terms age sex patient group included this case-control...

Escherichia coli serotype O157: H7 and E. O104: H4 are well known foodborne pathogens causing sever enteric illness. Using bacteriophages as biocontrol agents of some multidrug-resistant (MDR) bacteria has a great attention nowadays. This study aims to test the effect cocktail phages on growth MDR coli. Routine conventional PCR was used confirm identification isolates. Double-layered culture technique isolate from sewage water. Morphology bacteriophage described using transmission electron...

The widespread use of antimicrobials has increased the occurrence multidrug resistant microbes. commonest mechanism antimicrobial resistance in Enterobacteriaceae is production β-lactamases such as metallo-β-lactamases (MBL) and extended spectrum (ESBL). Few studies have used a molecular approach to characterize prevalence β-lactamases. Here, different was characterized by performing three multiplex PCRs targeting genes similar those described earlier publications. Antimicrobial...

Streptococcus pneumoniae causes life-threatening infections such as meningitis, pneumonia, and febrile bacteremia, particularly in young children. The increasing number of drug-resistant isolates has highlighted the necessity for intervening controlling disease. To achieve this, information is needed on serotype distribution patterns antibiotic resistance children.All cases invasive pneumococcal disease (IPD) children aged less than 15 yr recorded at King Khalid University Hospital, Saud...

Secretory N-acyl homoserine lactones (AHLs) mediate quorum sensing (QS) in bacteria. AHLs are shown to be inhibitory for an unrelated group of bacteria and might mimic host signalling elements, thereby subverting the regulatory events cells. This study investigated AHL produced by Acinetobacter baumannii analysed its effect on other bacterial species mammalian Chemically characterized had m/z value 325 with a molecular formula C18H31NO4 showed potential against Staphylococcus aureus....

Objective To evaluate changes in the expression of biofilm‐related genes when exposed to tobacco smoke and oxidative stress. Study Design Experimental, vitro. Setting Laboratories Rhinology Microbiology, University Pennsylvania. Subjects Methods Bacterial biofilm mass was measured using crystal violet staining measurement optical density. Biofilm‐related Pseudomonas aeruginosa PAO1 strain ( pilF, flgK, lasI, lasB, rhlA , algC ) were studied following repetitive exposure exogenous hydrogen...

Streptococcus pneumoniae causes life threatening infections and necessitate for impediment controlling disease; to conquer this, information is needed about serotype distribution patterns of antibiotic resistance. The present study was determine the S. isolated from entire age group individual correlate this with susceptibility. Cases pneumococcal have been reviewed serotyping Out 117 isolates 45 (39%) were penicillin-resistant, 84 (72%) erythromycin-resistant 100% co-trimoxazole resistant....

Multidrug resistance (MDR) of Acinetobacter baumannii increasingly jeopardizes the health care setting leading to substantial mortality and morbidity globally. During past decade, entirely resistant A. strains presented a real challenge clinicians posed difficulties in therapy. MDR baumannii– associated infections, with adverse clinical outcomes involving respiratory tract, blood, soft tissues, urinary central nervous system, significantly increases outlay infirmary. Being Gram-negative...