A5046129840- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Immunotherapy and Immune Responses
- Cancer Immunotherapy and Biomarkers
- Blood groups and transfusion
- Immune cells in cancer
- Platelet Disorders and Treatments
- Hemoglobinopathies and Related Disorders
- Diabetes and associated disorders
- COVID-19 Clinical Research Studies
- Inflammatory Biomarkers in Disease Prognosis
- Immune Response and Inflammation
- Chronic Lymphocytic Leukemia Research
- Iron Metabolism and Disorders
- Allergic Rhinitis and Sensitization
- Acute Myeloid Leukemia Research
- Reproductive System and Pregnancy
- Lysosomal Storage Disorders Research
- Multiple Myeloma Research and Treatments
- Asthma and respiratory diseases
- Cancer Cells and Metastasis
- Autoimmune and Inflammatory Disorders Research
- Galectins and Cancer Biology
- Long-Term Effects of COVID-19
- MicroRNA in disease regulation
Adelphi University
2025
Assiut University
2015-2024
Al-Azhar University
2023
Minia University
2007-2022
Children Cancer Hospital
2012-2019
King Saud University
2016
Background and aim The study aimed to determine whether the MPs levels platelet activation are affected by COVID-19 infection in both malignant non-malignant patients compared healthy individuals define their contribution associated coagulopathy relation of these other hematologic parameters. Patients methods We recruited 23 with reverse transcription polymerase chain reaction (RT-PCR) positive COVID-19, also, 19 patients, 20 volunteers were also enrolled for comparison. Blood samples...
Abstract Background Choroid plexus (CP) tumors are rare brain neoplasms that mainly affect the pediatric population. Unlike benign CP papilloma (CPP), carcinoma (CPC) is an aggressive cancer with a dismal survival rate. Despite chromosome-wide rearrangements, drivers of most remain elusive except recurrent alterations in TP53. Studies signaling dysregulation may bring biological understanding these malignancies. Previous studies implicated NOTCH tumors; we developed mouse models driven by...
Lung Cancer (LC) is a major cancer killer worldwide, and 5-yr survival extremely poor (≤15%), accentuating the need for more effective diagnostic therapeutic strategies. Studies have shown cell-free microRNAs (miRNAs) circulating in serum plasma with specific expression cancer, indicating potential of using miRNAs as biomarkers diagnosis therapy. This study aimed to identify differentially-expressed two Non-Small Cell (NSCLC) patients that might be clinically useful tool lung early...
Neutrophil-to-lymphocyte ratio (NLR) was introduced as a potential inflammatory marker in sickle cell disease (SCD). This study aimed to evaluate the impact of hydroxyurea (HU) treatment on value NLR and some mediators SCD.The hematological parameters clinical events were analyzed 35 children with SCD under HU followed up for 1 year 20 healthy controls. Enzyme-linked immunosorbent assay performed evaluation proinflammatory cytokines, including interleukin (IL) 6, IL-8, high-sensitivity...
We evaluated the effect of varicocelectomy on semen parameters and levels sperm DNA damage in infertile men. A total 75 men with varicocele 40 fertile (controls) were included this study. Semen analysis expressed as fragmentation index using acridine orange staining chromatin condensation test by flow cytometry assessed before 6 months after varicocelectomy. The patients also followed up for 1 year pregnancy outcome. significantly lower compared to controls (P < 0.05). Mean percentages...
Background: Accurate anthropometric measurements and critical analysis of growth data allow the clinician to promptly recognize children with short stature. The aim this study was determine frequency etiological factors causing stature among referred pediatric endocrinology clinic Assiut University Children’s Hospital, main tertiary care center in Upper Egypt. Methods: We conducted descriptive observational from May 2012 December 2015, analyze 637 (boys 354, girls 283) Evaluation included:...
Abstract Pseudomonas aeruginosa is the most common pathogenic gram-negative bacteria causing corneal ulcers globally. In severe cases, often after trauma and eye injury, destruction progresses rapidly may be completed within 24–48 h blindness. our preliminary work, we have established an ultrasensitive polyaniline (PANI)/gold nanoparticles (Au NPs)/indium tin oxide (ITO) modified sensor for rapid detection of pyocyanin (PYO) in P . infections with a linear range from 238 μM to 1.9 limit 500...
Regulatory B cells (Bregs) are unique subpopulations of with immune-regulating or immune-suppressing properties and play a role in peripheral tolerance. Due to the current limitations human Breg studies among periodontal diseases, present study, we tried analyze change circulating Bregs, pro-inflammatory, anti-inflammatory cytokines patients periodontitis. Peripheral blood from 55 stage 2 periodontitis 20 healthy controls was analyzed using flow cytometry evaluate frequency CD19+CD24+CD38+...
We aimed to determine the levels of follicular helper T (Tfh) and regulatory (Tfr) cells in COVID-19 patients whether their correlated with disease severity presence hyperglycemia. This study was carried out 34 hospitalized 20 healthy controls. Levels total circulating Tfh, inducible T-cell costimulator (ICOS)+ activated Tfr were assessed all participants by flow cytometry. Total CD4+CXCR5+ Tfh ICOS+Foxp3-activated increased ICOS+Foxp3+ decreased patients, especially diabetic those severe...
Hematopoietic stem cells (HSCs) and hematopoietic progenitor (HPCs) play a crucial role in the context of viral infections their associated diseases. The link between HSCs HPCs disease status COVID-19 patients is largely unknown. This study aimed to monitor kinetics contributions severe non-severe evaluate diagnostic performance differentiating healthy as well cases. Peripheral blood (PB) samples were collected from 48 patients, 16 recovered, 27 controls subjected deep flow cytometric...
This study aimed to investigate the oxidative stress, hypoxia biomarkers, and circulating microparticles (MPs) in β thalassemia major. The included 56 children with 46 healthy controls. Hypoxia stress total plasma fragmented DNA (fDNA) were detected by standard methods. MPs assessed flow cytometry. fDNA, higher antioxidant capacity (TAC) was lower patients than In splenectomized those who had complications, vascular endothelial growth factor (VEGF), malondialdehyde, endothelial, platelet,...
To our knowledge, no previous studies have focused on the immunomodulatory effects of fresh royal jelly (RJ) administration systemic lupus erythematosus (SLE) in humans. Our aim was to study effect RJ disease course children with SLE some immunological markers (CD4+ and CD8+ regulatory T cells lymphocytes apoptosis).This an open-label which 20 received 2 g freshly prepared daily, for 12 weeks.The percentages CD4+ CD25+high FOXP3+cells cells) CD8+CD25+high (CD8+ were significantly increased...
BACKGROUND: The bone marrow immunosuppressive microenvironment of AML patients sustains and modulates proliferation, survival drug resistance through deregulation both innate adaptive immune response. We aimed to investigate the level Tregs, expression Tim-3 on peripheral bl ood T cells, CD200 in myeloid blasts newly diagnosed with normal cytogenetics (AML-NC) their prognostic impact. PATIENTS AND METHODS: This study included 40 de novo AML-NC 20 healthy controls. Flow-cytometry was used for...
Abstract Background and aim Myeloid‐derived suppressor cells (MDSCs) play a pivotal role in tumor immunity induction of immune tolerance to variety antitumor effectors, including T lymphocytes. Herein, we tried evaluate the frequency clinical significance MDSCs different lymphocyte subsets hepatitis C virus (HCV)‐related hepatocellular carcinoma (HCC). Methods Four groups were enrolled; chronic HCV (CHC; n = 40), HCV‐related liver cirrhosis (n HCC (HCV‐HCC; 75), healthy control group 20)....
The inflammatory basis of diabetes mellitus directed the researchers' attention to immune system for better management and prevention complications. Metoclopramide (MCA; only US Food Drug Administration-approved gastroparesis) has ability restore function through increasing prolactin secretion. This study aimed test effect BSA/MCA nanoparticles (NPs) on modulating response.BSA/MCA NPs were fabricated by desolvation evaluated in vitro via measuring loading efficiency, particle size, surface...
Background: Lung cancer is one of the main human health threats. Survival lung patients depends on timely detection and diagnosis. Among genetic irregularities that control development progression, there are microRNAs (miRNAs). This study aimed to assess plasma level circulating miRNA-17 miRNA-222 as non-invasive markers in non-small-cell (NSCLC) patients. Patients methods: A total 40 with NSCLC 20 healthy controls who were matched terms age sex patient group included this case-control...
Growing evidence highlighted the primary role of immune system in disease course triple-negative breast cancer (TNBC). The study aim was to investigate expression PD-1 and CD39 on CD4+ CD8+ cells infiltrating tumor tissue compared their counterparts peripheral blood explore its association with characteristics, progression, prognosis females TNBC.
We aimed to examine the levels of lymphocyte subsets and regulatory T cells in patients with newly diagnosed immune thrombocytopenia (ITP) their correlation course ITP. The study included 40 pediatric acute ITP 30 controls. Lymphocytes were analyzed by flow cytometry. percentages CD19(+) CD8(+) significantly increased while that CD4(+) CD4(+)/CD8(+) ratio decreased. CD4(+)CD25(+High) forkhead box protein 3 (Foxp3(+)) expression Foxp3(+) decreased patients. Age, platelet count, mean volume...
This study aimed to analyze the frequency of peripheral Mo-myeloid-derived suppressor cells (Mo-MDSCs) in newly diagnosed CLL patients and correlate their level with other prognostic factors such as CD38 ZAP-70 clinical response survival outcomes these patients. Fifty 20 age-matched healthy controls were included this study. Flow cytometric detection ZAP 70, CD38, Mo-MDSCs was done. Mo-MDSC levels wer significantly higher (27.51 ± 1.70) than (16.79 0.66; p < .0001). Higher detected advanced...