A5022238882- SARS-CoV-2 and COVID-19 Research
- Viral gastroenteritis research and epidemiology
- Animal Virus Infections Studies
- Immunotherapy and Immune Responses
- Influenza Virus Research Studies
- Lanthanide and Transition Metal Complexes
- Analytical chemistry methods development
- Bacterial Infections and Vaccines
- Nanoparticle-Based Drug Delivery
- Monoclonal and Polyclonal Antibodies Research
- Hepatitis Viruses Studies and Epidemiology
- RNA Interference and Gene Delivery
- Respiratory viral infections research
University of North Carolina at Chapel Hill
2022-2024
Moderna Therapeutics (United States)
2021
Cornell University
2015-2017
Lipid nanoparticles (LNP) are effective delivery vehicles for messenger RNA (mRNA) and have shown promise vaccine applications. Yet there no published reports detailing how LNP biophysical properties can impact performance. In our hands, a retrospective analysis of mRNA in vivo studies revealed relationship between particle size immunogenicity mice using LNPs various compositions. To further investigate this, we designed series to systematically change without altering lipid composition...
Group 2B β-coronaviruses (sarbecoviruses) have caused regional and global epidemics in modern history. Here, we evaluate the mechanisms of cross-sarbecovirus protective immunity, currently less clear yet important for pan-sarbecovirus vaccine development, using a panel alphavirus-vectored vaccines covering bat to human strains. While vaccination does not prevent virus replication, it protects against lethal heterologous disease outcomes both severe acute respiratory syndrome coronavirus 2...
Antibodies perform both neutralizing and non-neutralizing effector functions that protect against certain pathogen-induced diseases. A human antibody directed at the SARS-CoV-2 Spike N-terminal domain (NTD), DH1052, was recently shown to be non-neutralizing, yet it protected mice cynomolgus macaques from severe disease. The mechanisms of NTD antibody-mediated protection are unknown. Here we show Fc mediate (non-nAb) MA10 viral challenge in mice. Though non-nAb prophylactic infusion did not...
Abstract The biodistribution of biodegradable nanoparticles can be difficult to quantify. We report a method using time resolved fluorescence (TRF) from lanthanide chelate minimize background autofluorescence and maximize the signal noise ratio detect nanoparticle distribution in mice. Specifically, antenna chelates containing europium were entrapped within composed polylactic acid-polyethylene glycol diblock copolymers. Tissue accumulation following intravenous injection was quantified TRF...
Two group 2B β-coronaviruses (sarbecoviruses) have caused regional and global epidemics in modern history. The mechanisms of cross protection driven by the sarbecovirus spike, a dominant immunogen, are less clear yet critically important for pan-sarbecovirus vaccine development. We evaluated cross-sarbecovirus protective immunity using panel alphavirus-vectored vaccines covering bat to human strains. While vaccination did not prevent virus replication, it protected against lethal...