A5052290692- Protein Structure and Dynamics
- Enzyme Structure and Function
- Advanced NMR Techniques and Applications
- NMR spectroscopy and applications
- Metabolomics and Mass Spectrometry Studies
- Advanced MRI Techniques and Applications
- Molecular spectroscopy and chirality
- RNA and protein synthesis mechanisms
- Reproductive Biology and Fertility
- Genomics and Phylogenetic Studies
- Assisted Reproductive Technology and Twin Pregnancy
- Growth Hormone and Insulin-like Growth Factors
- Mass Spectrometry Techniques and Applications
- Nanoparticle-Based Drug Delivery
- Antimicrobial Peptides and Activities
- Graphene and Nanomaterials Applications
- Chemical Synthesis and Analysis
- Analytical Chemistry and Chromatography
- Supramolecular Self-Assembly in Materials
- Cancer, Hypoxia, and Metabolism
- Glycosylation and Glycoproteins Research
- interferon and immune responses
- Immune Response and Inflammation
- Graphene research and applications
- Advanced biosensing and bioanalysis techniques
Indian Institute of Science Bangalore
2015-2024
Jain University
2022
Bangalore University
2014-2020
Resonance Research (United States)
2020
Solid State Physics Laboratory
2014
Kasturba Medical College, Manipal
2012
Manipal Academy of Higher Education
2012
University at Buffalo, State University of New York
2004-2010
Tata Institute of Fundamental Research
1999-2005
Joint Center for Structural Genomics
2005
Abstract Small molecule immune checkpoint inhibitors targeting PD-1 and other pathways may offer advantages including ease of dosing, ability to manage immune-related adverse events (irAEs) due their shorter pharmacokinetic exposure opportunity target more than one pathway for improving efficacy. Here we describe the identification characterization CA-170, an amino acid inspired small inhibitor PD-L1 VISTA derived from interface PD-L1. CA-170 exhibited potent rescue proliferation effector...
Abstract The recent rise of multidrug resistant microbial strains requires development new and novel therapeutic alternatives. In this study, we present a antibacterial system that comprises modified naturally abundant antimicrobial peptides in conjugation with silver nanoparticles. Further, propose simple route to incorporate cysteine residue either at the N- or C-terminal parent peptide. Tagging terminals not only enhances binding propensity resultant peptide nanoparticle, but also...
A G-matrix Fourier transform (GFT) NMR spectroscopy-based strategy for resonance assignment of proteins is described. Each the GFT experiments presented here rapidly affords four-, five-, or six-dimensional spectral information in combination with precise measurements chemical shifts. The resulting high content enables one to obtain nearly complete assignments by using only four experiments. For backbone amide proton detected “out-and-back” experiments, data collection was further...
Factors responsible for the folding of aromatic oligoamides with backbones rigidified by local three-center H-bonds were investigated. The stability was quantified half-lives amide proton-deuterium exchange reactions, which show that largely intact at room temperature in oligomer examined. This result is consistent our current and previous 2D NMR studies. overall helical conformation nonamer 1 found variable-temperature NOESY studies to be dynamic. As rose, end-to-end NOEs rapidly...
NMR-based metabolomics, which emerged along with mass spectrometry techniques, is the preferred method for studying metabolites in medical research and food industries. However, NMR techniques suffer from inherently low sensitivity, regardless of their superior reproducibility. To overcome this, we made two beneficial modifications: detuned probe to reach a position called "Spin Noise Tuning Optimum" (SNTO), replaced conventional cylindrical 5 mm tube an electric field component-optimized...
A standardized protocol enabling rapid NMR data collection for high-quality protein structure determination is presented that allows one to capitalize on high spectrometer sensitivity: a set of five G-matrix Fourier transform experiments resonance assignment based highly resolved 4D and 5D spectral information acquired in conjunction with single simultaneous 3D 15 N, 13 C aliphatic , aromatic -resolved [ 1 H, H]-NOESY spectrum providing H- H upper distance limit constraints. The was...
Sequence specific resonance assignment constitutes an important step towards high-resolution structure determination of proteins by NMR and is aided selective identification amino acid types. The traditional approach to labeling yields only the chemical shifts particular being selected does not help in establishing a link between adjacent residues along polypeptide chain, which for sequential assignments. An alternative method 'unlabeling' or reverse labeling, involves unlabeling types...
There has been growing interest in understanding energy metabolism human embryos generated using assisted reproductive techniques (ART) for improving the overall success rate of method. Using NMR spectroscopy as a noninvasive tool, we studied embryo to identify specific biomarkers assess quality their implantation potential. The study was based on estimation pyruvate, lactate and alanine levels growth medium, ISM1, used culture embryos. An involving 127 from 48 couples revealed that...
We report the first peptide based hDHFR inhibitors designed on basis of structural analysis dihydrofolate reductase (DHFR). A set peptides were rationally and synthesized using solid phase synthesis characterized nuclear magnetic resonance enzyme immunoassays. The best candidate among them, a tetrapeptide, was chosen molecular mechanics calculations evaluated in human lung adenocarcinoma cell line A549. It showed significant reduction proliferation an IC50 82 µM obtained. interaction with...
The dynamic interaction of ubiquitin with graphene oxide can be modulated by changing the oxidation degree oxide.
A protocol for high-quality structure determination based on G-matrix Fourier transform (GFT) NMR is presented. Five through-bond chemical shift correlation experiments providing 4D and 5D spectral information at high digital resolution are performed resonance assignment. These combined with a newly implemented (4,3)D GFT NOESY experiment which encodes of 15N/15N-, 13Calipahtic/15N-, 13Caliphatic/13Caliphatic-resolved [1H,1H]-NOESY in two subspectra, each containing one component doublets...
In recent years, magnetic core–shell nanoparticles have received widespread attention due to their unique properties that can be used for various applications.
J-proteins are obligate cochaperones of Hsp70s and stimulate their ATPase activity via the J-domain. Although functions have been well understood in context Hsp70s, additional co-evolved "physiological functions" still elusive. We report here solution structure mechanism novel iron-mediated functional roles human Dph4, a type III J-protein playing vital role diphthamide biosynthesis normal development. The NMR Dph4 reveals two domains: conserved J-domain CSL-domain connected flexible...
DNA-binding protein domains (DBDs) sample diverse conformations in equilibrium facilitating the search and recognition of specific sites on DNA over millions energetically degenerate competing sites. We hypothesize that DBDs have co-evolved to sense exploit strong electric potential from array negatively charged phosphate groups DNA. test our hypothesis by employing intrinsically disordered DBD cytidine repressor (CytR) as a model system. CytR displays graded increase structure, stability...