A5014344399- Advanced NMR Techniques and Applications
- Cancer Immunotherapy and Biomarkers
- Advanced MRI Techniques and Applications
- Cancer, Hypoxia, and Metabolism
- Metabolomics and Mass Spectrometry Studies
- Reproductive Biology and Fertility
- Immunotherapy and Immune Responses
- Nanoplatforms for cancer theranostics
- Atomic and Subatomic Physics Research
- NMR spectroscopy and applications
- Assisted Reproductive Technology and Twin Pregnancy
- Electron Spin Resonance Studies
- Cancer, Lipids, and Metabolism
- Solid-state spectroscopy and crystallography
- Metabolism, Diabetes, and Cancer
- Immune cells in cancer
- Mitochondrial Function and Pathology
- Cancer Research and Treatments
- Ferroptosis and cancer prognosis
- Chemokine receptors and signaling
- Sperm and Testicular Function
- Nanoparticle-Based Drug Delivery
- CAR-T cell therapy research
- Radiopharmaceutical Chemistry and Applications
- MRI in cancer diagnosis
The University of Texas MD Anderson Cancer Center
2016-2025
Scripps MD Anderson Cancer Center
2024
University of Arkansas for Medical Sciences
2024
Institute on Aging
2024
Indian Institute of Science Bangalore
2012-2023
Baylor College of Medicine
2019
Roswell Park Comprehensive Cancer Center
2019
Center for Theoretical Biological Physics
2019
Texas A&M University
2019
Rice University
2019
Abstract Despite the clinical success of T-cell checkpoint blockade, most patients with cancer still fail to have durable responses immunotherapy. The molecular mechanisms driving blockade resistance, whether preexisting or evolved, remain unclear. To address this critical knowledge gap, we treated B16 melanoma combination CTLA-4, PD-1, and PD-L1 a Flt3 ligand vaccine (≥75% curative), isolated tumors resistant therapy, serially passaged them in vivo same treatment regimen until they...
There has been growing interest in understanding energy metabolism human embryos generated using assisted reproductive techniques (ART) for improving the overall success rate of method. Using NMR spectroscopy as a noninvasive tool, we studied embryo to identify specific biomarkers assess quality their implantation potential. The study was based on estimation pyruvate, lactate and alanine levels growth medium, ISM1, used culture embryos. An involving 127 from 48 couples revealed that...
In recent years, magnetic core–shell nanoparticles have received widespread attention due to their unique properties that can be used for various applications.
Porous silicon nanoparticles have recently garnered attention as potentially-promising biomedical platforms for drug delivery and medical diagnostics. Here, we demonstrate porous contrast agents 29 Si magnetic resonance imaging. Size-controlled were synthesized by magnesiothermic reduction of silica surface activated further functionalization. Particles hyperpolarized via dynamic nuclear polarization to enhance their MR signals; the particles demonstrated long spin-lattice relaxation (T1 )...
Laser assisted zona hatching (LAH) is a routinely used therapeutic intervention in reproductive technology for patients with poor prognosis. However, results are not conclusive demonstrating the benefits of improving pregnancy rate. Recent observations on LAH induced genetic instability animal embryos prompted us to look into effects laser human preimplantation embryo quality and metabolic uptake using high resolution nuclear magnetic resonance (NMR) technology. This experimental prospective...
Early development of certain mammalian embryos is protected by complex checkpoint systems to maintain the genomic integrity. Several metabolic pathways are modulated in response genetic insults cells. The present study investigated relationship between integrity, embryo metabolites and developmental competence preimplantation stage mouse with aim identify early biomarkers which can predict embryonic integrity using spent medium profiling NMR spectroscopy. Embryos carrying induced DNA lesions...
Silicon particles have garnered attention as promising biomedical probes for hyperpolarized 29Si magnetic resonance imaging and spectroscopy. However, due to the limited levels of hyperpolarization nanosized silicon particles, microscale primarily been focus dynamic nuclear polarization (DNP) applications, including in vivo (MRI). To address these current challenges, we developed a facile synthetic method partially 29Si-enriched porous nanoparticles (NPs) (160 nm) examined their usability...
Abstract An NMR‐based approach for rapid characterization of translational diffusion molecules has been developed. Unlike the conventional method acquiring a series 2D 13 C and 1 H spectra, proposed involves single NMR spectrum, which can be acquired in minutes. Using this method, it was possible to detect presence intermediate oligomeric species diphenylalanine solution during process its self‐assembly form nanotubular structures.
Peroxisome proliferator-activated receptor delta (PPARD) is a nuclear known to play an essential role in regulation of cell metabolism, proliferation, inflammation, and tumorigenesis normal cancer cells. Recently, we found that newly generated villin-PPARD mouse model, which PPARD overexpressed villin-positive gastric progenitor cells, demonstrated spontaneous development large, invasive tumors as the mice aged. However, downstream metabolism tumor cells elusive. The aim present study was...
While aerobic glycolysis, or the Warburg effect, has for a long time been considered hallmark of tumor metabolism, recent studies have revealed far more complex picture. Tumor cells exhibit widespread metabolic heterogeneity, not only in their presentation effect but also nutrients and pathways they are dependent on. Moreover, can switch between different phenotypes response to environmental cues therapeutic interventions. A framework analyze observed heterogeneity plasticity is, however,...
Hyperpolarized magnetic resonance spectroscopy enables quantitative, non-radioactive, real-time measurement of imaging probe biodistribution and metabolism in vivo. Here, we investigate report on the development characterization hyperpolarized acetylsalicylic acid (aspirin) its use as a nuclear (NMR) probe. Aspirin derivatives were synthesized with single- double-
"Tumor-educated platelets" have recently generated substantial interest for the diagnosis of cancer. We hypothesized that tumor educated platelets from patients with brain tumors will reflect altered metabolism compared to healthy volunteers. Here, in a pilot study, we employed nuclear magnetic resonance (NMR) spectroscopy demonstrate obtained
Abstract Introduction: Pancreatic cancer is difficult to diagnose due its asymptomatic presentation at early stages. Therefore, there an unmet need for non-invasive imaging markers that can help identify the aggressive pancreatic lesions time point. One of most promising biomarkers conversion hyperpolarized (HP) pyruvate lactate1-3. With HP metabolic imaging, will be monitored among different premalignant models timepoints as well tested against pancreatitis, a known confounder in clinic....
OBJECTIVES/GOALS: Acidity and the lactate-to-pyruvate ratio correlate with immunotherapy resistance. AcidoCEST MRI hyperpolarized magnetic resonance spectroscopy (HP-MRS) measure extracellular pH ratio. We will establish a baseline for these biomarkers then observe changes after combination esomeprazole immunotherapy. METHODS/STUDY POPULATION: used multiple melanoma models created via serial in vivo passage under immunotherapeutic pressure (FVAX, CTLA-4, PD-1, PD-L1). four of corresponding...
Peroxisome proliferator-activated receptor delta (PPARD) is a ligand-dependent nuclear transcription factor that regulates multiplicity of pathophysiological processes vital to cell metabolism. Recent discovery PPARD overexpressed in villin-positive gastric progenitor cells, demonstrated spontaneous development large, invasive tumors as the mice aged. These unique animal models allowed us address knowledge gap PPARD-regulated downstream metabolic changes and determine significance...