Platycodon grandiflorum is a flowering plant that used in traditional medicine for treating pulmonary and respiratory disorders. It exerts various pharmacological effects, including immunomodulatory anti-cancer activities. The purpose of this study was to confirm the vitro vivo immune-enhancing effects P. extract (PGE) on splenocytes isolated from cyclophosphamide (CP)-induced immunosuppressed rats.For analysis, were treated with PGE at doses along CP. Cell viability measured by WST-1 assay,...

Soy isoflavones are popular ingredients with anti-adipogenic and anti-lipogenic properties. The properties of genistein well-known, but those genistin glycitein remain unknown, daidzein characterized by contrasting data. Therefore, the purpose our study was to investigate effects daidzein, glycitein, genistein, on adipogenesis lipogenesis in 3T3-L1 cells. Proliferation preadipocytes unaffected it affected 50 100 µM for 48 h. Among four isoflavones, only markedly suppressed lipid accumulation...

Transient receptor potential channel ankryn 1 (TRPA1) expressed in the gastrointestinal tract is associated with gastric motility, emptying, and food intake. In this study, we investigated effects of methyl syringate, a specific selective TRPA1 agonist, on intake, gut hormone levels imprinting control region (ICR) mice. The administration syringate suppressed cumulative intake emptying. addition, treatment ruthenium red (RR), general cation blocker, HC-030031, antagonist, inhibited...

Abstract Although the role of agrin in formation neuromuscular junction is well established, other functions for have remained elusive. The present study was undertaken to assess neurite outgrowth mediated by heparin‐binding growth factor basic fibroblast (FGF‐2), which we shown previously bind with high affinity and that has been mediate from a number neuronal cell types. Using both an established line, PC12 cells, primary chick retina cultures, find potentiates ability FGF‐2 stimulate...

The antidiabetic effect of the Citrus junos Tanaka (also known as yuja or yuzu) was examined. Ethanol extract peel (YPEE) significantly stimulated 2-[ N -(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino]-2-deoxy-D-glucose (2-NBDG) uptake in C2C12 myotubes. However, ethanol pulp (YpEE) and water (YPWE) (YpWE) did not stimulate glucose uptake. In addition, peroxisome proliferator-activated receptor gamma (PPAR-<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML"...

Cinnamyl alcohol (CA) is an aromatic compound found in several plant-based resources and has been shown to exert anti-inflammatory anti-microbial activities. However, the anti-adipogenic mechanism of CA not sufficiently studied. The present study aimed investigate effect on regulation adipogenesis. As evidenced by Oil Red O staining, Western blotting, real-time PCR (RT-PCR) analyses, treatment (6.25-25 μM) for 8 d significantly inhibited lipid accumulation a concentration-dependent manner...

Lemon myrtle essential oil (LMEO), a popular food-flavoring and perfume, has anti-inflammatory antioxi-dant properties, contains citral in abundance. Citral-enriched oils exhibit anti-obesity effects; how-ever, the effect of LMEO on adipogenesis precise mechanism have not been elucidated. This study evaluated anti-adipogenic activity commercial its components, geranial, neral (a mixture geranial neral), 3T3-L1 cell differentiation identified citral. Treatment preadipocytes with various...

Isoeugenol (IEG), a natural component of clove oil, possesses antioxidant, anti-inflammatory, and antibacterial properties. However, the effects IEG on adipogenesis have not yet been elucidated. Here, we showed that blocks in 3T3-L1 cells at an early stage. inhibits lipid accumulation adipocytes concentration-dependent manner reduces expression mature adipocyte-related factors including PPARγ, C/EBPα, FABP4. treatment different stages inhibited adipocyte differentiation by suppressing stage,...

Although the five basic taste qualities—sweet, sour, bitter, salty and umami—can be recognized by respective gustatory system, interactions between these qualities are often experienced when food is consumed. Specifically, umami has been investigated in terms of whether it enhances or reduces other modalities. These studies, however, based on individual perception not a molecular level. In this study we umami-sweet using compounds including monosodium glutamate (MSG), 5'-mononucleotides...

Atopic dermatitis (AD) is a chronic inflammatory skin disease that associated with intestinal microflora. Since specific probiotics may have better efficacy for AD, we determined the of Pediococcus acidilactici SRCM102024 (PA) treating AD in HaCaT cells and NC/Nga mice explored mechanism action. AD-like pathology was induced dorsal Nc/Nga by local exposure to 2,4-dinitrochlorobenzene (DNCB). In AD-lesion mice, PA low-, medium- high-dosages (5 × 10E6, 5 10E7 10E8 CFU/kg bw, respectively)...

Obese Asians are more susceptible to metabolic diseases than obese Caucasians of the same body mass index (BMI). We hypothesized that genetic variants associated with obesity risk interact lifestyles middle-aged and elderly adults, possibly allowing development personalized interventions based on genotype. aimed examine this hypothesis in a large city hospital-based cohort Korea. The participants cancers, thyroid diseases, chronic kidney disease, or brain-related were excluded. divided into...

Context: Boehmeria nivea (Linn.) Gaudich (Urticaceae), a natural herb, has long history of treating several diseases including wound healing. However, the anti-inflammatory effect B. not been investigated.

Skeletal muscle atrophy is characterized by reduced function and size. Oxidative stress contributes to but can be treated with antioxidants. This study investigated the antioxidant activity of a castor oil plant leaf (Ricinus communis L.) extract (RC) its effects on atrophy. Rutin was identified as major compound among thirty compounds in RC via LC-MS/MS found inhibit dexamethasone (DEX)-induced mitochondrial oxidative stress. Rutin-rich showed DPPH ABTS radical scavenging activities...

Exercise is an effective strategy to prevent sarcopenia, but high physical inactivity in the elderly requires alternative therapeutic approaches. mimetics are compounds that simulate beneficial effects of exercise on skeletal muscles. However, toxicity and adverse raise serious concerns. We aimed search novel plant-based alternatives activate induced-signaling. used open databases luciferase assays identify plant-derived exercise-induced signaling compared its efficacy mild intensity...

Abstract This study presents an approach that can be used to search for lead peptide candidates, including unconstrained structures in a recognized sequence. was performed using the design of competitive inhibitor 3‐hydroxy‐3‐methylglutaryl CoA reductase (HMGR). In previous constrained peptides, head‐to‐tail cyclic structure as model linear analog searches peptides with close active conformation. Analysis conformational space occupied by suggests analogical applied finding sequence via...

Imipramine (IMI) is a tricyclic synthetic antidepressant that used to treat chronic psychiatric disorders, including depression and neuropathic pain. IMI also has inhibitory effects against various cancer types, prostate cancer; however, the mechanism of its anticancer activity not well understood. In present study, we investigated antimetastatic anti-invasive in metastatic castration-resistant PC-3 cells, with an emphasis on serine/threonine protein kinase AKT-mediated nuclear factor kappa...

This study investigated the effects of conditioned medium (CM) from canine amniotic membrane-derived MSCs (cAMSCs) on dog sperm cryopreservation. For this purpose, flow cytometry analysis was performed to characterize cAMSCs. The CM prepared cAMSCs subjected proteomic for identification proteins present in medium. Sperm samples were treated with freezing supplemented 0%, 5%, 10%, and 15% CM, kinetic parameters evaluated after 4-6 h chilling at 4 °C select best concentration before proceeding...

The effects of commercial vegetable oils and duck skin on quality characteristics a reduced-fat meat emulsion were examined. cooking loss, stability, hardness lower for emulsions preemulsified with (P < 0.05) than the control. Storage modulus (G′) loss (G″) treated corn, grape seed, soy, olive similar to values control; highest G′ G″ reported coconut oil. Myofibril protein solubility was oil 0.05). Replacing pork back fat different emulsification may impart superior emulsion. We recommend...

In age-related macular degeneration, N-retinylidene-N-retinylethanolamine (A2E) accumulates in retinal pigment epithelium (RPE) cells and generates oxidative stress, which further induces cell death. Polyphenols are well known for their antioxidant beneficial effects on vision. Chrysanthemum boreale Makino (CB) flowers, contain flavonoids, have activity. We hypothesized that polyphenols ethanolic extracts of CB (CBE) its fractions suppressed A2E-mediated ARPE-19 damage, a human RPE line. CBE...