A5004951996- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- CRISPR and Genetic Engineering
- Cancer-related gene regulation
- Protein Kinase Regulation and GTPase Signaling
- Cytokine Signaling Pathways and Interactions
- Animal Virus Infections Studies
- RNA modifications and cancer
- Cancer Cells and Metastasis
- Virus-based gene therapy research
- Immunotherapy and Immune Responses
- Epigenetics and DNA Methylation
- Glycosylation and Glycoproteins Research
- Aquaculture disease management and microbiota
- Monoclonal and Polyclonal Antibodies Research
- HIV Research and Treatment
- Invertebrate Immune Response Mechanisms
- Chromosomal and Genetic Variations
Max Planck Institute of Immunobiology and Epigenetics
2018-2023
Instituto Gulbenkian de Ciência
2013-2016
Cytidine deaminases of the AID/APOBEC family catalyze C-to-U nucleotide transitions in mRNA or DNA. Members APOBEC3 branch are involved antiviral defense, whereas AID contributes to diversification antibody repertoires jawed vertebrates via somatic hypermutation, gene conversion, and class switch recombination. In extant jawless vertebrate, lamprey, two members implicated generation diversity variable lymphocyte receptors (VLRs). Expression studies linked CDA1 CDA2 genes assembly VLRA/C...
The antibodies of jawless vertebrates consist leucine-rich repeat arrays encoded by somatically assembled VLRB genes. It is unknown how the incomplete germline loci are converted into functional antibody genes during B lymphocyte development in lampreys. In Lampetra planeri larvae lacking cytidine deaminase CDA2 gene, assembly fails, whereas T lineage-associated VLRA and VLRC antigen receptor gene assemblies occur normally. Thus, acts a cell lineage-specific fashion to support somatic...
Recombination-Activating Genes (RAG) 1 and 2 form the site specific recombinase that mediates V(D)J recombination, a process of DNA editing required for lymphocyte development responsible their diverse repertoire antigen receptors. Mistargeted RAG activity associates with genome alteration is various lymphoid tumors. Moreover several non-lymphoid tumors express ectopically. A practical powerful tool to perform quantitative assessment score putative RAG-Recognition signal sequences (RSS) in...
DNA methylation is a universal epigenetic mechanism involved in regulation of gene expression and genome stability. The maintenance methylase DNMT1 ensures that patterns are faithfully transmitted to daughter cells during cell division. Because loss lethal, pan-organismic analysis function lacking. We identified new recessive dnmt1 alleles medaka zebrafish and, guided by the structures mutant proteins, generated variant mouse Dnmt1. Each three missense mutations studied here distorts...
The RAG recombinase is a domesticated transposable element co-opted in jawed vertebrates to drive the process of so-called V(D)J recombination, which hallmark adaptive immune system produce antigen receptors. targets, namely, Recombination Signal Sequences (RSS), are rather long and degenerated sequences, highlights ability interact with wide range target including outside receptor loci. recognition such cryptic targets by threatens genome integrity promoting aberrant DNA as observed...