A5029414873- Genomics and Chromatin Dynamics
- Cancer Genomics and Diagnostics
- Proteoglycans and glycosaminoglycans research
- RNA modifications and cancer
- Cervical Cancer and HPV Research
- Cancer-related gene regulation
- Single-cell and spatial transcriptomics
- Developmental Biology and Gene Regulation
- Breast Lesions and Carcinomas
- Pancreatic function and diabetes
- Cancer, Hypoxia, and Metabolism
- Mesenchymal stem cell research
- Lung Cancer Treatments and Mutations
- Chromosomal and Genetic Variations
- CRISPR and Genetic Engineering
- Epigenetics and DNA Methylation
- Microtubule and mitosis dynamics
- Cell Image Analysis Techniques
- Planarian Biology and Electrostimulation
- RNA Interference and Gene Delivery
- Acute Myeloid Leukemia Research
- Pluripotent Stem Cells Research
- Pulmonary Hypertension Research and Treatments
- Hippo pathway signaling and YAP/TAZ
- Silk-based biomaterials and applications
Yale University
2016-2025
University of New Haven
2022-2023
Northeastern University
2017-2021
University of Calgary
2016
Intermountain Medical Center
2016
University of Utah
2016
Vanderbilt University
2016
Calgary Laboratory Services
2016
Johns Hopkins University
2016
Centre Hospitalier de l’Université de Montréal
2016
The prevailing dogma for morphological patterning in developing organisms argues that the combined inputs of transcription factor networks and signalling morphogens alone generate spatially temporally distinct expression patterns. However, metabolism has also emerged as a critical developmental regulator
Abstract Three-dimensional (3D) genome organization becomes altered during development, aging, and disease 1–23 , but the factors regulating chromatin topology are incompletely understood currently no technology can efficiently screen for new regulators of multiscale organization. Here, we developed an image-based high-content screening platform (Perturb-tracing) that combines pooled CRISPR screen, a cellular barcode readout method (BARC-FISH), tracing. We performed loss-of-function in human...
In mammalian cells, gene copy number is tightly controlled to maintain expression and genome stability. However, a common molecular feature across cancer types oncogene amplification, which promotes progression by drastically increasing the of tumor-promoting genes. For example, in tyrosine kinase inhibitor (TKI)-resistant lung adenocarcinoma (LUAD), amplification occurs over 40% patients' tumors. Despite prevalence TKI-resistant tumors, mechanisms facilitating are not fully understood....
Ambystoma mexicanum, the axolotl salamander, is a classic model organism used to study vertebrate regeneration. It assumed that axolotls regenerate most tissues, but exploration of lung regeneration has not been performed until now.Unlike blastema-based response during appendage regeneration, amputation led organ-wide proliferation. Pneumocytes and mesenchymal cells responded injury by increased proliferation throughout injured lung, which recovery in mass morphology 56 days post-amputation....
ABSTRACT The induction of new lung tissue after disease or trauma has the potential to save lives and transform patient outcomes. Ambystoma mexicanum, axolotl salamander, is a classic model organism used study vertebrate regeneration, primarily limb amputation. While it hypothesized that axolotls regenerate all their tissues, exploration regeneration not been performed until now. Proliferation injury was observed be global response, suggesting utilizes compensatory mechanism, in contrast...
Abstract Although three-dimensional (3D) genome structures are altered in cancer cells, little is known about how these changes evolve and diversify during progression. Leveraging genome-wide chromatin tracing to visualize 3D folding directly tissues, we generated atlases of murine lung pancreatic adenocarcinoma. Our data reveal stereotypical, non-monotonic, stage-specific alterations heterogeneity, compaction, compartmentalization as cancers progress from normal preinvasive ultimately...
Efforts to elucidate novel mechanisms of lung regeneration have the potential change lives and transform health care. The axolotl salamander, Ambystoma mexicanum, has long been held as a model organism vertebrate regeneration, particularly in limbs. It is thought that they regenerate all their tissues, but exploration not previously characterized. In this study, we conducted experiments demonstrating axolotls are capable significant after amputation distal third lung. We shown importance...
Unlike humans, axolotl salamanders are capable of restoring sensory and motor function after spinal cord injury. Upon tail amputation or transection, endogenous neural stem cells (NSCs) increase proliferation differentiate into multiple glial cell types the central peripheral nervous system. How NSCs transition from a resting state to regenerative coordinate proper differentiation is poorly understood. Here, we study global changes that occur in injury as well specific gene transcription NSC...
Abstract Lung cancer is the leading cause of cancer-related death worldwide and affects over 2 million people each year. In lung adenocarcinoma (LUAD), somatic activating mutations epidermal growth factor receptor (EGFR) occur in approximately 15% patients. The first-line therapy for patients with EGFR-mutant LUAD administration osimertinib, an EGFR tyrosine kinase inhibitor (TKI). While many patients’ tumors initially respond to osimertinib treatment, resistance inevitably develops most...