A5062032064- Genomics and Chromatin Dynamics
- X-ray Diffraction in Crystallography
- Crystallization and Solubility Studies
- T-cell and B-cell Immunology
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Immune Cell Function and Interaction
- Single-cell and spatial transcriptomics
- Computational Drug Discovery Methods
- RNA Research and Splicing
- RNA and protein synthesis mechanisms
- RNA modifications and cancer
- Chromosomal and Genetic Variations
- Advanced biosensing and bioanalysis techniques
- Ion channel regulation and function
- Advanced Drug Delivery Systems
- Epigenetics and DNA Methylation
- Analytical Chemistry and Chromatography
- Drug Solubulity and Delivery Systems
- Potassium and Related Disorders
- Diet, Metabolism, and Disease
- Probiotics and Fermented Foods
- DNA Repair Mechanisms
- Synthesis of Organic Compounds
- Microbial Metabolites in Food Biotechnology
- Fermentation and Sensory Analysis
Yale University
2019-2025
University of New Haven
2025
Merck & Co., Inc., Rahway, NJ, USA (United States)
2014-2024
State Key Laboratory of Food Science and Technology
2021-2024
Nanchang University
2021-2024
Friedrich-Alexander-Universität Erlangen-Nürnberg
2021
Dalian University
2019-2021
Dalian University of Technology
2018-2021
Central China Normal University
2021
United States Military Academy
2020
Artemisin combination therapy (ACT) is the main treatment option for malaria, which caused by intracellular parasite Plasmodium. However, increased resistance to ACT highlights importance of finding new drugs. Recently, aspartic proteases Plasmepsin IX and X (PMIX PMX) were identified as promising drug targets. In this study, we describe dual inhibitors PMIX PMX, including WM382, that block multiple stages Plasmodium life cycle. We demonstrate PMX a master modulator merozoite invasion direct...
The three-dimensional architecture of the genome affects genomic functions. Multiple architectures at different length scales, including chromatin loops, domains, compartments, and lamina- nucleolus-associated regions, have been discovered. However, how these structures are arranged in same cell they mutually correlated types mammalian tissue largely unknown. Here, we develop Multiplexed Imaging Nucleome Architectures that measures multiscale folding, copy numbers numerous RNA species,...
The subcellular localization of mRNAs plays a pivotal role in biological processes, including cell migration. For instance, β-actin mRNA and its associated RNA-binding protein (RBP), ZBP1/IGF2BP1, are recruited to focal adhesions (FAs) support localized synthesis, crucial for However, whether other RBPs also localize at FAs remains unclear. Here, we identify hundreds that enriched (FA-mRNAs). FA-mRNAs share characteristics with stress granule (SG) found ribonucleoprotein (RNP) complexes the...
Fluorescence in situ hybridization (FISH) is a powerful method to visualize the spatial positions of specific genomic loci and RNA species. Recent technological advances have leveraged FISH these features highly multiplexed manner. Notable examples include chromatin tracing, error-robust (MERFISH), imaging nucleome architectures (MINA), sequential single-molecule FISH. However, one obstacle broad adoption methods complexity probe design. In this paper, we introduce an easy-to-use, versatile,...
Correct 3D genome organization is essential for the proper functioning of genome. Recent advances in image-based genomics techniques have enabled direct tracing chromatin folding and multiplexed imaging nucleome architectures single cells several important biological systems. Here, we discuss these future directions genomics. The spatial genomic DNA nucleus controls functions including gene expression regulation, replication, mutation, repair, recombination, crucially involved many...
Abstract Three-dimensional (3D) genome organization becomes altered during development, aging, and disease 1–23 , but the factors regulating chromatin topology are incompletely understood currently no technology can efficiently screen for new regulators of multiscale organization. Here, we developed an image-based high-content screening platform (Perturb-tracing) that combines pooled CRISPR screen, a cellular barcode readout method (BARC-FISH), tracing. We performed loss-of-function in human...
Abstract Background Topologically associating domains (TADs) are important building blocks of three-dimensional genome architectures. The formation TADs has been shown to depend on cohesin in a loop-extrusion mechanism. Recently, advances an image-based spatial genomics technique known as chromatin tracing lead the discovery cohesin-independent TAD-like structures, also single-cell domains, which highly variant self-interacting with boundaries that occasionally overlap TAD but tend differ...
Computer-supported collaborative concept mapping (CSCCM) integrates technology and to support students' knowledge understanding, much research on the behavioral patterns involved in CSCCM activities has been conducted. However, there is limited understanding of differences between students with different levels collaboration perception. This study examined impact perceptions their activity. A total 36 individuals from same university participated this study. The findings suggested that...
Abstract The spatial organization of the genome plays a vital role in maintaining cell type-specific chromatin structures and regulating gene expression. three-dimensional (3D) can be measured by sequencing-based techniques such as Hi-C on ensemble level or imaging-based tracing single-molecule level. Chromatin is multiplexed DNA fluorescence situ hybridization (FISH) technique that directly trace 3D positions genomic loci along individual chromosomes single cells. By designing primary...
The imidazo-[1,2-a]-pyrazine (1) is a dual inhibitor of Aurora kinases A and B with modest cell potency (IC50 = 250 nM) low solubility (5 μM). Lead optimization guided by the binding mode led to acyclic amino alcohol 12k (SCH 1473759), which picomolar (TdF K d Aur 0.02 nM 0.03 improved (phos-HH3 inhibition IC50 25 intrinsic aqueous (11.4 mM). It also demonstrated efficacy target engagement in human tumor xenograft mouse models.
Abstract Non‐alcoholic fatty liver disease (NAFLD) can progress to the more serious non‐alcoholic steatohepatitis (NASH), characterized by inflammatory injury and fibrosis. The pathogenic basis of NAFLD progressing NASH is currently unknown, but growing evidence suggests MD2 (myeloid differentiation factor 2), an accessory protein TLR4, important signalling component contributing this disease. We evaluated effectiveness specific inhibitor, L6H21, in reducing a relevant high‐fat diet (HFD)...
Mushroom derived phytochemical has become the promising agent to treat inflammatory bowel disease (IBD). Here, we investigated effect of flavonoids from Agrocybe aegirita (AAF) on dextran sodium sulfate‐induced colitis. Our results showed that had a certain physical signs in mice (improving weight loss mice, reducing DAI index and spleen mice). AAF could also significantly reduce shortening colon, improve level tissue damage colon inflammation. Besides, alleviate status including levels...
A cross 1,3-dipolar cycloaddition of two different ylides between C,N-cyclic azomethine imines with an in situ-generated nonstabilized ylide from N-benzyl precursor was realized. The reactions afforded a clean and facile access to diverse fused tricyclic 1,2,4-hexahydrotriazines high yields (up 96%). chemical structures the typical compounds were confirmed by X-ray single-crystal structure analysis.
We study how the design of work environments in open laboratories affects engagement visitors innovation processes. For this purpose, we present evidence from an embedded case five projects laboratory Central Europe. Theoretically, build on concept boundary objects and investigate which types boundaries are addressed by different environments. identify three interaction schemes very differently. find that as spaces for between organisations communities can give account multiple perspectives...
A rare example of crystal form-dependent, gamma radiation-induced degradation is presented. Islatravir known to exist in several polymorphic forms, but only one these forms shows the generation a specific dimer product under irradiation. Extended irradiation studies demonstrated that crystalline an appreciable rate formation. Additionally, this not observed form other forced stress conditions. We present structural elucidation impurity and rationalize its form-dependent based on analysis...
Abstract Messenger RNA (mRNA) compartmentalization within the cytosol is well-recognized as a key mechanism of local translation-mediated regulation protein levels, but whether such localization could be means exercising non-coding mRNA function unknown. Here, we explore functions for mRNAs associated with focal adhesions (FAs), cellular structures responsible mediating cell adhesion and response to changes in extracellular matrix (ECM). Using high-throughput single molecule imaging genomic...