A5100711373- Mass Spectrometry Techniques and Applications
- Hemoglobin structure and function
- Microtubule and mitosis dynamics
- SARS-CoV-2 and COVID-19 Research
- Cancer-related Molecular Pathways
- Hemoglobinopathies and Related Disorders
- SARS-CoV-2 detection and testing
- Diabetes, Cardiovascular Risks, and Lipoproteins
- Heme Oxygenase-1 and Carbon Monoxide
- Computational Drug Discovery Methods
- Protein Structure and Dynamics
- Metabolomics and Mass Spectrometry Studies
- Advanced Glycation End Products research
- Iron Metabolism and Disorders
- Photosynthetic Processes and Mechanisms
- Diabetes Management and Research
- Hormonal and reproductive studies
- Neonatal Health and Biochemistry
- Cancer therapeutics and mechanisms
- Alzheimer's disease research and treatments
- COVID-19 Clinical Research Studies
- Molecular Biology Techniques and Applications
- Erythrocyte Function and Pathophysiology
- Porphyrin Metabolism and Disorders
- Microbial metabolism and enzyme function
Indian Institute of Science Education and Research Kolkata
2020-2024
St. John's National Academy of Health Sciences
2012-2021
Institute of Molecular Medicine
2015
St. John's University
2012-2015
Bangalore University
2015
Indian Association for the Cultivation of Science
2014
Merck & Co., Inc., Rahway, NJ, USA (United States)
2010
Indian Institute of Science Bangalore
2007
Dynamics of excited state proton transfer (ESPT) in the lysosome region live lung cells (normal and cancer) is studied by picosecond time-resolved confocal microscopy. For this, we used a fluorescent probe, pyranine (8-hydroxy-pyrene-1,3,6-trisulfonate, HPTS). From colocalization HPTS with lysotracker dye (lysotracker yellow), confirmed that resides for both cells. The diffusion coefficient (Dt) was obtained from fluorescence correlation spectroscopy (FCS). Dt, viscosity estimated to be ∼40...
The SoxXAYZB(CD)2 -mediated pathway of bacterial sulfur-chemolithotrophy explains the oxidation thiosulfate, sulfide, sulfur and sulfite but not tetrathionate. Advenella kashmirensis, which oxidizes tetrathionate to sulfate, besides forming it as an intermediate during thiosulfate oxidation, possesses a soxCDYZAXOB operon. Knock-out mutations proved that only SoxBCD is involved in A. kashmirensis whereas thiosulfate-to-tetrathionate conversion Sox independent. Expression two glutathione...
ABSTRACT Cervical cancer, which is the fourth most common gynaecological cancer across globe, has a poorly understood molecular pathogenesis and etiology. Current methods of diagnosis are based on cytology, histology presence Human Papillomavirus (HPV). Shortcomings these lie with poor quality smears very in Papanicolaou (pap) smears, limited sensitivity terms early detection, dependence HPV, etc. Due to its high non-targeted approach, Matrix Assisted Laser Desorption Ionization Imaging Mass...
The imidazo-[1,2-a]-pyrazine (1) is a dual inhibitor of Aurora kinases A and B with modest cell potency (IC50 = 250 nM) low solubility (5 μM). Lead optimization guided by the binding mode led to acyclic amino alcohol 12k (SCH 1473759), which picomolar (TdF K d Aur 0.02 nM 0.03 improved (phos-HH3 inhibition IC50 25 intrinsic aqueous (11.4 mM). It also demonstrated efficacy target engagement in human tumor xenograft mouse models.
NP-Fe<sub>4</sub>(P<sub>2</sub>O<sub>7</sub>)<sub>3</sub>significantly improved hemoglobin level in iron-deficient rats and Fetuin-B showed differential biological response across NP doses through plasma proteomics.
Glutathionyl hemoglobin, a post-translationally modified form of has been reported to serve as marker oxidative stress in several clinical conditions. This modification causes perturbations the hemoglobin functionality by increasing oxygen affinity and reducing cooperativity. Moreover, glutathionylation sickle was lead significant reduction propensity sickling erythrocytes. The root cause above functional abnormality is not known detail, crystal structure molecule yet be discovered. In this...
To gain insight into the underlying mechanisms of various biological events, it is important to study structure-function correlation proteins within cells. Structural probes used in spectroscopic tools investigate protein conformation are similar across all proteins. Therefore, structural studies restricted purified vitro and these findings extrapolated cells correlate their functions vivo. However, due cellular complexity, vivo environments radically different. Here, we show a novel way...
The infection caused by Severe Acute Respiratory Syndrome–CoronaVirus-2 (SARS-CoV-2) resulted in a pandemic across the globe with huge death toll. symptoms from SARS-CoV2 appear somewhat similar to SARS-CoV-1 that appeared early 21st century but infectivity is far higher for SARS-CoV-2. virus attaches itself exposed human epithelial cells through spike protein. Recently discovered crystal structure of complex protein SARS-CoV-2 angiotensin-converting enzyme 2 (ACE2) receptor indicated binds...
The infection caused by Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) resulted in a pandemic with huge death toll and economic consequences. virus attaches itself to the human epithelial cells through noncovalent bonding of its spike protein angiotensin-converting enzyme-2 (ACE2) receptor on host cell. Based silico studies we hypothesized that perturbing functionally active conformation reduction solvent accessible disulfide bonds, thereby disintegrating structural...
Besides multiple life-threatening diseases like lung cancer and cardiovascular disease, cigarette smoking is known to produce hypoxia, a state of inadequate oxygen supply tissues. Hypoxia plays pivotal role in the development chronic obstructive pulmonary disease. Smoking during pregnancy imposes risk for unborn child. In addition carbon monoxide, conjugation para-benzoquinone (pBQ), derived from smoke, with human hemoglobin (HbA) was also reported contribute hypoxia. fact, pBQ more alarming...
Glycated hemoglobin (GHb) is the indicator of long‐term glycemic index an individual. GHb formed by irreversible modification N‐terminal α‐amino group β globin chain with glucose via Amadori rearrangement. Cation exchange chromatography exploits difference in surface charges between and native (HbA 0 ) for their separation quantification. However, condensation specific to primary amino groups. Therefore, structural characterization synthesized vivo essential as multiple glycation may...
Nutritional anemia is a significant public health issue with 50-80% prevalence in Indian children. Fortification of food, specifically milk, iron potential approach to increase dietary intake. Ferric pyrophosphate [Fe4(P2O7)3] organoleptically neutral and less soluble acid medium and, further, has low bioavailability milk. However, since ascorbic potent enhancer absorption, the coadministration Fe4(P2O7)3 might enhance absorption iron. We evaluated effect on from an fortified milk beverage...
<p>The infection caused by Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) resulted in a pandemic with huge death toll and economic consequences. The virus attaches itself to the human epithelial cells through noncovalent bonding of its spike protein angiotensin-converting enzyme-2 (ACE2) receptor on host cell. We hypothesized that perturbing functionally active conformation reduction solvent accessible disulfide bond, thereby disintegrating structural architecture, may be...
Abstract Electrostatic attraction between α and β globin chains holds the subunits together in a tetrameric human hemoglobin molecule (α 2 ). Compared to normal chains, affinity of mutant chain its partner might be different genetic variants hemoglobin. This leads an unequal abundance variant heterozygous samples, even though rates synthesis both are same. The aforementioned affinities across various assessed by quantification forms tetramers present sample. In study, exploiting mass...