A5012898672- Advanced biosensing and bioanalysis techniques
- Nanoparticle-Based Drug Delivery
- Supramolecular Self-Assembly in Materials
- Nanoplatforms for cancer theranostics
- Microtubule and mitosis dynamics
- RNA Interference and Gene Delivery
- Graphene and Nanomaterials Applications
- Ubiquitin and proteasome pathways
- Mitochondrial Function and Pathology
- Photodynamic Therapy Research Studies
- Alzheimer's disease research and treatments
- ATP Synthase and ATPases Research
- Click Chemistry and Applications
- Nanocluster Synthesis and Applications
- 14-3-3 protein interactions
- Advanced Nanomaterials in Catalysis
- Graphene research and applications
- Lipid Membrane Structure and Behavior
- Antimicrobial Peptides and Activities
- Carbon and Quantum Dots Applications
- Molecular Sensors and Ion Detection
- Nanofabrication and Lithography Techniques
- Porphyrin and Phthalocyanine Chemistry
- Photosynthetic Processes and Mechanisms
- Polydiacetylene-based materials and applications
Indian Institute of Science Education and Research Kolkata
2024-2025
Ulsan National Institute of Science and Technology
2018-2024
Indian Institute of Chemical Biology
2013-2021
Government of the Republic of Korea
2019-2021
Academy of Scientific and Innovative Research
2016-2017
Council of Scientific and Industrial Research
2016-2017
Abstract Targeted drug delivery using nanoparticles can minimize the side effects of conventional pharmaceutical agents and enhance their efficacy. However, translating nanoparticle-based into clinical applications still remains a challenge due to difficulty in regulating interactions on interfaces between biological systems. Here, we present targeting strategy for incorporated with supramolecularly pre-coated recombinant fusion protein which HER2-binding affibody combines glutathione- S...
Metal-organic framework (MOF) nanoparticles with high porosity and greater tunability have emerged as new drug delivery vehicles. However, premature release still remains a challenge in the MOF system. Here, we report an enzyme-responsive, polymer-coated gatekeeper system using hyaluronic acid (HA) PCN-224 nanoMOF. The external surface of nanoMOF can be stably covered by HA through multivalent coordination bonding between Zr cluster carboxylic HA, which acts gatekeeper. allows selective...
Abstract Nanomedicines exploit the unusual physicochemical and biological properties of nanoparticles, including their nanoscopic size, high surface‐area‐to‐volume ratio, easy surface modification, biocompatibility. Among various therapeutic diagnostic applications nanomedicines, cancer‐targeted drug delivery systems (DDS) have been found to be a promising area research. Effective DDS rely on two major factors: slow sustained release chemotherapeutic drugs targeted tumor sites. Both...
Abstract Indolicidin (IR13), a 13‐residue antimicrobial peptide from the cathelicidin family, is known to exhibit broad spectrum of activity against various microorganisms. This inhibits bacterial DNA synthesis resulting in cell filamentation. However, precise mechanism remains unclear and requires further investigation. The central PWWP motif IR13 provides unique structural element that can wrap around, thus stabilize, duplex B‐type structures. Replacements Trp‐Trp pair with Ala‐Ala,...
Metal-organic framework (MOF) nanoparticles have recently emerged as a promising vehicle for drug delivery with high porosity and feasibility. However, employing MOF-based system remains challenge due to the difficulty in controlling interfaces of particles biological environment. In this paper, protein corona-blocked Zr6 -based MOF (PCN-224) are presented targeted cancer therapy efficiency. The unmodified PCN-224 surface is precoated glutathione transferase (GST)-fused targetable affibody...
Lysosomes remain powerful organelles and important targets for cancer therapy because cell proliferation is greatly dependent on effective lysosomal function. Recent studies have shown that membrane permeabilization induces death an way to treat by bypassing the classical caspase-dependent apoptotic pathway. However, most lysosome-targeted anticancer drugs very low selectivity cells. Here, we show intra-lysosomal self-assembly of a peptide amphiphile as technique overcome this problem. We...
Senolytics, which eliminate senescent cells from tissues, represent an emerging therapeutic strategy for various age-related diseases. Most senolytics target antiapoptotic proteins, are overexpressed in cells, limiting specificity and inducing severe side effects. To overcome these limitations, we constructed self-assembling targeting with intracellular oligomerization system. Intracellular aryl-dithiol-containing peptide occurred only inside the mitochondria of due to selective localization...
A novel neuro-compatible peptide-based hydrogel has been designed and developed, which contains microtubule stabilizing neuroprotective short peptide. This shows strong three-dimensional cross-linked fibrillary networks, can capture water molecules. Interestingly, this serves as excellent biocompatible soft material for 2D 3D (neurosphere) neuron cell culture provides stability of key cytoskeleton filaments such actin. Remarkably, it was observed that slowly enzymatically degrades releases...
Recent advances in supramolecular chemistry research have led to the development of artificial chemical systems that can form self-assembled structures imitate proteins involved regulation cellular function. However, intracellular polymerization operate inside living cells been seldom reported. In this study, we developed an intramitochondrial polymerization-induced self-assembly system for regulating fate cancer cells. It showed polymeric disulfide formation occurred due high reactive...
Dynamics of excited state proton transfer (ESPT) in the lysosome region live lung cells (normal and cancer) is studied by picosecond time-resolved confocal microscopy. For this, we used a fluorescent probe, pyranine (8-hydroxy-pyrene-1,3,6-trisulfonate, HPTS). From colocalization HPTS with lysotracker dye (lysotracker yellow), confirmed that resides for both cells. The diffusion coefficient (Dt) was obtained from fluorescence correlation spectroscopy (FCS). Dt, viscosity estimated to be ∼40...
Blue, green, orange-red, red and NIR emitting gold quantum clusters have been prepared in aqueous media by using a bioactive peptide glutathione (reduced) at physiological pH. Matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOF MS) analyses show that the core structure sizes of five different are Au7 (blue), Au16 (green), Au19 (orange-red), Au21 (red) Au22 (NIR). The photo-stability pH-stability these measured, photo-stable against continuous UV...
Targeted drug delivery systems based on metal-organic frameworks (MOFs) have progressed tremendously since inception and are now widely applicable in diverse scientific fields. However, translating MOF agents directly to targeted remains a challenge due the biomolecular corona phenomenon. Here, we observed that supramolecular conjugation of antibodies surface particles (MOF-808) via electrostatic interactions coordination bonding can reduce protein adhesion biological environments show...
To address the issue of nonspecific biodistribution a chemotherapeutic drug, stable [2]pseudorotaxane complexes (PK@CAOPP and PR@CAOPP) are used to demonstrate proof concept. Cationic -PPh3 + moiety in CAOPP allows specific localization PK@CAOPP/ PR@CAOPP mitochondrial membrane (MM). Electrostatic interaction between cationic LysinePK or ArgininePR negatively charged phosphoesterCAOPP functionality favours strong adduct formation. The ALP-induced hydrolytic cleavage phosphoester cancer cells...
Fluorescent gold nanoclusters (AuNCs) capped with lysozymes are used to deliver the anticancer drug doxorubicin cancer and noncancer cells. Doxorubicin-loaded AuNCs cause highly selective efficient killing (90 %) of breast cells (MCF7) (IC50 =155 nm). In contrast, (MCF10A) by doxorubicin-loaded is only 40 % =4500 By using a confocal microscope, fluorescence spectrum decay were recorded inside cell. The maxima (at ≈490-515 nm) lifetime (≈2 ns), correspond Au10-13 . intracellular release from...
Time resolved confocal microscopy indicates that the cytoplasmic lipid droplets (CLDs) in live cells (normal and cancer lung cells) are less polar, exhibit slower diffusion (motility) solvation dynamics than cytoplasm. The number of CLDs a human cell (A549) is ∼10 times higher non-cancer fibroblast (WI38). This may result accumulation non-polar signaling agents cell.
An ideal nano drug delivery agent must be potent enough to carry high dose of therapeutics and competent in targeting specific cell interest, having adequate optimized physiochemical properties biocompatibility. Carrying differentially polar simultaneously will make them superior their class. However, it is enormous challenge the researchers find such a unique nanocarrier engineer all above-mentioned features into it. In this manuscript, we have shown for first time that apoferritin (Apf)...
Mitochondria-targeting nucleopeptide (MNP) can sequester ATP by self-assembling with ATP. A small nanosized MNP/ADP complex forms a large assembly Thus, intramitochondrial co-assembly causes stress structures and apoptosis.
Tubulin is the key cytoskeleton component, which plays a crucial role in eukaryotic cell division. Many anticancer drugs have been developed targeting tubulin surface. Recently, it has shown that few polyhydroxy carbohydrates perturb polymerization. Cyclodextrin (CD), carbohydrate, extensively used as delivery vehicle for of hydrophobic to cancer cell. However, interaction CD with intracellular components not addressed before. In this Article, we first time α-CD interacts close vinblastine...
Lyso-CORM is very stable under dark conditions CO and singlet oxygen generation ( 1 O 2 ) was effectively triggered light irradiation conditions.
Development of green light responsive fluorescein-based photoremovabl protecting group and nanoparticle formulation for controlled release bioactive molecules with real-time monitoring abilit.
Herein, we report a novel hexapeptide, derived from activity dependent neuroprotective protein (ADNP), that spontaneously self-assembles to form antiparallel β-sheet structure and produces nanovesicles under physiological conditions. This peptide not only strongly binds with β-tubulin in the taxol binding site but also microtubule lattice vitro as well intracellular networks. Interestingly, it shows inhibition of amyloid fibril formation upon co-incubation Aβ following an interesting...
Many anticancer drugs are developed for the treatment of cancer from natural sources. Photosystem I (PSI), a protein complex present in chloroplast, is involved photosynthesis and generates reactive oxygen species (ROS) plant. Here, we used ROS generation property PSI therapy. We show that can enter into different kinds cell like human lung carcinoma (A549) mouse melanoma (B16F10) lines generate inside cells. It inhibits proliferation causes apoptotic death also induces apoptosis through...