A5059623265- Mosquito-borne diseases and control
- Viral Infections and Vectors
- Parvovirus B19 Infection Studies
- Malaria Research and Control
- Viral Infections and Outbreaks Research
- Cuban History and Society
- Vector-Borne Animal Diseases
- Biosensors and Analytical Detection
- Zoonotic diseases and public health
- Dermatological and COVID-19 studies
- Dengue and Mosquito Control Research
- Histiocytic Disorders and Treatments
- Fire effects on ecosystems
- Vector-borne infectious diseases
- Maternal and Neonatal Healthcare
- Health, Nursing, Elderly Care
- Phytoplasmas and Hemiptera pathogens
- Urologic and reproductive health conditions
Fundação de Medicina Tropical
2014-2024
Endesa (Spain)
2021
Universidade do Estado do Amazonas
2012
Instituto de Medicina Tropical
2004
National Institute of Amazonian Research
1999
We characterized 3 autochthonous dengue virus serotype cases and 1 imported case from 2 states in the North South Regions of Brazil, 15 years after Brazil's last outbreak involving this serotype. also identified a new Asian lineage recently introduced into Americas, raising concerns about future outbreaks.
Abstract We report dengue virus type 4 (DENV-4) in Amazonas, Brazil. This was isolated from serum samples of 3 patients treated at a tropical medicine reference center Manaus. All cases were confirmed by serologic and molecular tests; 1 patient co-infected with DENV-3 DENV-4.
Oropouche fever is the second most frequent arboviral infection in Brazil, surpassed only by dengue. virus (OROV) causes large and explosive outbreaks of acute febrile illness cities villages Amazon Central-Plateau regions. Cerebrospinal fluid (CSF) samples from 110 meningoencephalitis patients were analyzed. The RNA extracted was submitted to reverse transcription-polymerase chain reaction sequencing identify OROV. Three CSF showed presence OROV causing central nervous system (CNS). These...
Malaria and dengue are the most prevalent vector-borne diseases worldwide represent major public health problems. Both endemic in tropical regions, propitiating co-infection. Only few co-infection cases have been reported around world, with insufficient data so far to enhance understanding of effects clinical presentation severity.A cross-sectional study was conducted (2009 2011) hospitalized patients acute febrile syndrome Brazilian Amazon. All were submitted thick blood smear PCR for...
INTRODUCTION: Manaus, the capital city of state Amazon with nearly 2 million inhabitants, is located in middle rain forest and has suffered dengue outbreaks since 1998. METHODS: In this study, blood samples were investigated using reverse transcriptase-polymerase chain reaction (RT-PCR), aimed at identifying virus serotypes. RESULTS: Acute phase sera from 432 patients tested for presence virus. Out patients, 137 (31.3%) found to be positive. All four serotypes observed. CONCLUSIONS: The...
Human Parvovirus B19 (B19V) infection in asymptomatic Immunodeficiency Virus (HIV) patients often presents as an exanthematous illness with joint pain, potentially leading to the misdiagnosis dengue or other diseases. This study a case of B19V/HIV co-infection patient initially suspected having who was undergoing treatment at reference hospital Manaus, Amazonas. The sample tested for HIV and virus then stored -80°C. HIV-positive dengue-negative, B19V DNA recently detected. should be included...
Objetivo: relatar a detecção molecular do B19V em tecido cardíaco de uma paciente pediátrica, com suspeita clínica dengue. Métodos: o DNA viral foi extraído mediante coração, seguido pela reação PCR e pelo sequenciamento nucleotídeos. Resultado: DNAB19V detectado na amostra (AM199801), negativa para dengue; análise da sequência nucleotídica indicou genótipo 1 B19V. Conclusão: este achado mostra subnotificação áreas endêmicas dengue, destacando importância investigação laboratorial associada...
Em 1998, a Fundação de Medicina Tropical/Instituto Tropical do Amazonas implementou o sistema vigilância para síndromes febris agudas indiferenciadas, com propósito manter ativa e passiva na Amazônia Ocidental, Brasil, permitindo identificar diagnosticar os agentes etiológicos causadores febres agudas. O diagnóstico foi realizado através estudos sorológicos detecção anticorpos IgM, utilizando-se técnicas ELISA (Enzyme-linked-immunosorbent assay) kits comerciais. Foram analisadas 8.557...
The natural co-infection with dengue virus can occur in highly endemic areas where different serotypes have been observed for many years. We report here four cases of DENV-3/DENV-4 detected by serological and molecular tests among 674 patients acute undifferentiated fever from the tropical medicine reference center Manaus City, Brazil, between 2005 2010. Analysis sequences obtained indicated presence genotype 3 1 DENV-3 DENV-4 respectively.
Hantavirus disease is caused by the hantavirus, which an RNA virus belonging to family Bunyaviridae. anthropozoonotic infection transmitted through inhalation of aerosols from excreta hantavirus-infected rodents. In county Itacoatiara in state Amazonas (AM), Brazil, first human cases hantavirus pulmonary and cardiovascular syndrome were described July 2004. These followed two fatal cases, one municipality Maués 2005 another 2007. this study, we investigated antibody levels a population 1,731...
Abstract In 2023, three autochthonous DENV-3 cases were detected in Roraima and one imported case Paraná, fifteen years after the last outbreak Brazil. Phylogenetic analyses confirmed all belonging to a new Asian lineage recently introduced Americas, raising concerns about future large dengue outbreaks this region.
Proof of concept an immunoassay, which is easy to implement, for rapid Dengue virus (DENV) serotype diagnosis, in the early infection stage, reported. The four-layer assay immobilized onto a thin gold film and relies on low cost, disposable polymer biochip optical surface plasmon resonance sensing detection. protocol comprises Neutravidin-Biotin mediated monoclonal antibody (MAB) attachment as functionalized element. Formation MAB-DENV complex results pronounced thickness change that...
INTRODUCTION Mayaro virus (MAYV) was found in Pará state, Brazil, 1955. Since then, sporadic outbreaks have occurred different regions of the country. METHODS: Serum sample were collected from 49 individuals 2016 and initially tested for dengue (DENV) by real-time (RT) polymerase chain reaction (PCR). DENV-negative samples MAYV Oropouche (OROV) multiplexed RT quantitative PCR. RESULTS: All negative DENV OROV, but detected four samples. CONCLUSIONS: Differential diagnoses acute febrile...
Several orthobunyaviruses are important arthropod-borne pathogens, responsible for a variety of diseases in humans, from acute febrile illness to encephalitis.We collected serum samples series dengue suspected cases Tefé, mid-size city located the interior Amazonas state, Brazil. Viral RNA extraction was performed, and specimens were tested virus using RT-PCR. Thirty negative further Mayaro (MAYV) Oropouche (OROV) an RT-qPCR protocol previously described. Positive characterized by MegaBLAST...
Introduction In Manaus, the first autochthonous cases of dengue fever were registered in 1998. Since then, diagnosed by isolation viruses 1, 2, 3, and 4. Methods One hundred eighty-seven mosquitoes collected with BioGents (BG)-Sentinel traps 15 urban residential areas Northern Zone Manaus processed molecular tests. Results Infections 4 a case co-infection 2 3 identified. Conclusions These findings corroborate detection clinical samples reinforce need for epidemiological surveillance Health...
Human parvovirus B19 (B19V) is a common pathogen, which on infection causes variety of clinical conditions from benign self-limiting exanthematous disease and other similar pathologies to fetal death.We collected 341 serum samples between the first fourth day after onset symptoms all patients suspected dengue fever who were attended at Regional Hospital Tefé. Initially, screened for malaria by blood smear test negative sent Fundação de Medicina Tropical Doutor Heitor Vieira Dourado (FMT-HVD)...
Investigation of 1,107 cases exanthematous diseases in Manaus allowed the identification first 47 human parvovirus B19 city. Parvovirus was characterized by a combination signs and symptoms such as fever, chronic headache, arthralgia, myalgia exanthema. The frequency exanthema higher individuals less than fifteen years old. In adults, fever arthropathy were most frequent symptoms. highest number registered 1999. Regarding gender, male sex predominant among infected younger old, female...