A5022466461- RNA Interference and Gene Delivery
- Advanced biosensing and bioanalysis techniques
- Retinal Development and Disorders
- Lipid Membrane Structure and Behavior
- Cellular transport and secretion
- DNA and Nucleic Acid Chemistry
- Connexins and lens biology
- Venomous Animal Envenomation and Studies
- Advanced Polymer Synthesis and Characterization
- Neonatal Respiratory Health Research
- Retinal Diseases and Treatments
- Cytokine Signaling Pathways and Interactions
- Probiotics and Fermented Foods
- Medicinal plant effects and applications
- Nanopore and Nanochannel Transport Studies
- Neuroscience and Neuropharmacology Research
- Pharmacological Effects of Natural Compounds
- Marine Invertebrate Physiology and Ecology
- Immune Response and Inflammation
- Protein Kinase Regulation and GTPase Signaling
- Carbon dioxide utilization in catalysis
- Neuroscience of respiration and sleep
- Sphingolipid Metabolism and Signaling
- Nanocluster Synthesis and Applications
- RNA regulation and disease
Sofia University "St. Kliment Ohridski"
2015-2025
Institut de la Vision
2010
Centre National de la Recherche Scientifique
2010
Inserm
2010
Sorbonne Université
2010
University of Hohenheim
2005
Autosomal-recessive retinitis pigmentosa (arRP) was recently associated with mutations in a novel gene EYS, spanning over 2 Mb, making it the largest known expressed human eye. The purpose of this study to establish prevalence and nature EYS clinically well-characterized cohort 239 sporadic arRP French cases. Direct sequencing performed 186 subjects for whom had previously been excluded by applying microarray technology. We mostly identified total 29 patients: Fifteen were predicted create...
Abstract Chlorogenic (5‐CQA), 1,5‐, 3,5‐, 4,5‐ and 3,4‐dicaffeoylquinic (DCQA) acids were identified quantified in the methanol extracts of Inula oculus‐christi L., I. bifrons aschersoniana Janka var. , ensifolia conyza ( Griess .) DC. germanica L. by HPLC analysis. The amount 5‐CQA varied from 5.48 to 28.44 mg/g DE highest content was detected . 1,5‐DCQA (4.05–55.25 DE) most abundant dicaffeoyl ester quinic acid followed 3,5‐DCQA, 4,5‐DCQA 3,4‐DCQA. extract showed total phenolic...
Polymer micelles represent one of the most attractive drug delivery systems due to their design flexibility based on a variety macromolecular synthetic methods. The environmentally safe chemistry in which use or generation hazardous materials is minimized has an increasing impact polymer-based nanosystems. In this work, solvent-free green procedure was applied for preparation amphiphilic diblock copolymer consisting biodegradable hydrophobic poly(acetylene-functional carbonate) and...
Mutations in BEST1 gene, encoding the bestrophin-1 (Best1) protein are associated with macular dystrophies. Best1 is predominantly expressed retinal pigment epithelium (RPE), and inserted its basolateral membrane. We investigated cellular localization polarized MDCKII cells of disease-associated mutant proteins to study specific sorting motifs Best1. Real-time PCR western blots for endogenous expression MDCK were performed. constructs generated using site-directed mutagenesis transfected...
Comblike polyethylenimines with varying degrees of polymerization both the main and side chains as well different grafting densities were evaluated gene delivery vectors. They able to condense linear plasmid DNA into nanosized polyplex particles dimensions surface potentials in 130-330 nm -30 +15 mV ranges, respectively, depending on amine/phosphate (N/P) ratio. The polyplexes remained stable aqueous buffer solutions from several hours up days. moderate colloidal stability was also...
Abstract The present contribution is focused on feasibility of using comb‐like copolymers polyethylenimine with poly(2‐ethyl‐2‐oxazoline) (LPEI‐comb‐PEtOx) varying grafting densities and degrees polymerization PEI PEtOx to deliver DNA molecules into cells. form small well‐defined particles at elevated temperatures, which are used as platforms for binding condensing DNA. electrostatic interactions between result in formation sub‐100 nm polyplex narrow size distribution different morphology...
Spherical nucleic acids (SNAs) have gained significant attention due to their unique properties allowing them overcome the challenges that face current nanocarriers used for gene therapies. The aim of this study is synthesize and characterize polymer-oligonucleotide conjugates different architecture evaluate possibility forming SNAs with biodegradable cores. Initially, two types azide (multi)functional polyester-based (co)polymers were successfully synthesized characterized. In next step,...
The synergistic effect of drug and gene delivery is expected to significantly improve cancer therapy. However, it still challenging design suitable nanocarriers that are able load simultaneously anticancer drugs nucleic acids due their different physico-chemical properties. In the present work, an amphiphilic block copolymer comprising a biocompatible poly(ethylene glycol) (PEG) multi-alkyne-functional biodegradable polycarbonate (PC) was modified with number poly(2-(dimethylamino)ethyl...
Best1 protein (also known as bestrophin-1) is a highly conservative protein, member of the bestrophin family anion channels. It product BEST1 gene and expressed predominantly on basolateral plasma membrane retinal pigment epithelium cells in human retina. The exact functions are still under discussion, but its role an channel, regulator Ca2+ homeostasis development eye have been proposed. Mutations associated with several ocular diseases, named Bestrophinopathies (Best vitelliform macular...
Interleukin-6 (IL-6) is a multifunctional cytokine that important in inflammatory processes, cell differentiation and growth.Pulmonary surfactant protein-lipid complex, which reduce surface tension at the alveolar air-liquid interface, thereby preventing lung collapse. Surfactant protein A (SP-A) most abundant of four proteins (SP-A, SP-B, SP-C SP-D). It binds to macrophages, stimulates their chemotaxis serves as an opsonin phagocytosis.Low SP-A levels are associated with increased...
Lamium album L. is a perennial herb widely used in folk medicine. It possesses wide spectrum of therapeutic activities (anti-inflammatory, astringent, antiseptic, antibiotic, antispasmodic, antioxidant and anti-proliferative). Preservation medicinal plant could be done by vitro propagation to avoid depletion from their natural habitat. important know whether extracts plants grown possess similar properties as vivo. For these reasons, it examine changes the composition secondary metabolites...
An original hybrid biomacromolecule, consisting of a lipid-mimetic residue and 21-base long nucleic acid strand, spontaneously self-associates co-associates with other lipids into acid-grafted vesicular structures.
Abstract The present work describes the development, characterization, and in vitro evaluation of novel poly(L‐lysine)‐based polyplexes as nonviral gene delivery systems. Initially, a well‐defined hybrid block copolymer comprising poly(ethylene glycol) methacrylate) (POEGMA) poly(L‐lysine) (PLL) blocks was successfully synthesized characterized. shows high ability to condense DNA into stable aqueous media with sizes approx. 100 nm. nanoplexes were evaluated for cellular toxicity A549...
Misfolded amyloid beta (Aβ) peptides aggregate and form neurotoxic oligomers. Membrane mitochondrial damages, calcium dysregulation, oxidative stress, fibril deposits are among the possible mechanisms of Aβ cytotoxicity. Galantamine (GAL) prevents apoptosis induced by mainly through ability to stimulate allosterically α7 nAChRs regulate cytosolic concentration. Here, we examined cytoprotective effects two GAL derivatives, namely compounds
Spherical nucleic acids have emerged as a class of nanostructures, exhibiting wide variety properties, distinctly different from those linear acids, and plethora applications in therapeutics diagnostics. Herein, we report on preparation 3D acid prepared by self-assembly polymer–oligonucleotide conjugates. The latter are obtained grafting multiple alkyne-functionalized oligonucleotide strands onto azide-modified homo-, block, random (co)polymers chloromethylstyrene via initiator-free click...
Human bestrophin-1 protein (hBest1) is a transmembrane channel associated with the calcium-dependent transport of chloride ions in retinal pigment epithelium as well glutamate and GABA nerve cells. Interactions between hBest1, sphingomyelins, phosphatidylcholines cholesterol are crucial for hBest1 association cell membrane domains its biological functions. As plays key role formation lipid rafts, motional ordering lipids modeling/remodeling lateral structure, we examined effect different...