A5083094256- Microbial Inactivation Methods
- Microfluidic and Bio-sensing Technologies
- Toxin Mechanisms and Immunotoxins
- Carcinogens and Genotoxicity Assessment
- Bee Products Chemical Analysis
- RNA Interference and Gene Delivery
- Genetically Modified Organisms Research
- Plant Virus Research Studies
- Advanced biosensing and bioanalysis techniques
- Forensic and Genetic Research
- Pesticide and Herbicide Environmental Studies
- Electrostatic Discharge in Electronics
- Cutaneous Melanoma Detection and Management
- Dye analysis and toxicity
- Molecular Biology Techniques and Applications
- Botany, Ecology, and Taxonomy Studies
- CAR-T cell therapy research
- Listeria monocytogenes in Food Safety
- Sperm and Testicular Function
- Electromagnetic Fields and Biological Effects
- Viral Infectious Diseases and Gene Expression in Insects
- Nanoparticle-Based Drug Delivery
- Plant Disease Management Techniques
- Fungal Plant Pathogen Control
- Medical and Biological Ozone Research
Institute of Molecular Biology
2005-2014
Bulgarian Academy of Sciences
2005-2014
University Specialized Hospital for Active Treatment of Endocrinology
2011-2012
Eisai (Japan)
2005
Electrochemotherapy using rectangular biphasic stimuli and bursts of stimuli, as compared to monophasic pulses obtained by capacitor discharge, are at least the same efficacy. The use small surface electrodes in dermatological treatment yields electrode-skin impedances range 200-400 ohms, with being almost entirely resistive. Therefore, generator output resistances can be 10 20 ohms. a single burst 50+50 μsec pulses, an overall duration about 7 msec, is well tolerated patients, causing just...
Comblike polyethylenimines with varying degrees of polymerization both the main and side chains as well different grafting densities were evaluated gene delivery vectors. They able to condense linear plasmid DNA into nanosized polyplex particles dimensions surface potentials in 130-330 nm -30 +15 mV ranges, respectively, depending on amine/phosphate (N/P) ratio. The polyplexes remained stable aqueous buffer solutions from several hours up days. moderate colloidal stability was also...
Different kinds of additives are widely applied in food industry. The rationale for their use is preservation, coloring or sweetening diverse foods. Though it has been proven that some possess cytotoxic effect they still used practice. As a justification being low concentration application. We have the method Comet assay to detect minimal concentrations at which group selected could damage DNA. Five substances, commonly added foods and one pharmaceutical drugs showed DNA damaging effectively...
Electrochemotherapy is based on electroporation, which creates aqueous pathways in the cell membrane as a result of applied short intensive electric field usually generated by high voltage electrical pulses. The phenomenon enhances simultaneous administration cytotoxic drugs. Recently, several researchers forewarn some adverse effects that could occur with electrochemotherapy tumors located close to heart.We developed equipment Chemipulse III, consisting portable low-cost electroporator, QRS...
We report on novel gene delivery vector systems based hybrid polymer-magnetic micelles. The micelles were prepared by codissolution of hydrophobically surface modified iron oxide and amphiphilic polystyrene-b-poly(quaternized 2-vinylpyridine) block copolymer (PS-b-P2QVP) in organic solvent. After extensive dialysis against water, with positively charged hydrophilic corona PQVP hydrophobic PS core prepared, which magnetic nanoparticles randomly distributed. used to form complexes linear...
Comet assay gain its popularity because it is a fast and relatively easy-to-perform method for assessing damages in DNA. The two persisting variants of the method: alkaline neutral, lead to some doubts which one would be suitable certain purpose. Here we present comparison between versions developed by us Yeast Assay (YCA). Since DNA can quite diverse, i.e. single- or double- stranded cuts, oxidative damages, photodimers, etc., warn that YCA has applied with attention level understanding...
Electrochemotherapy could be considering as a good method for treatment of cutaneous and subcutaneous tumor lesions. Here we report the case 59 year-old woman with melanoma malignum cutis, who developed after axillary dissection, metastasis not appropriate surgical operation. BCG vaccine resulted in local control disease, complete response treated lesion.
Propolis is a natural product with different biological activities. Current research focused on its ability to suppress tumor growth by induction of apoptosis. However, selective influence normal and transformed cells not fully studied yet.Our previous investigations determined the McCoy-Plovdiv cell line as model system for in vitro examination propolis. In present study we analysed ethanol extracts propolis from region East Rodopi mountains final concentrations 0,001; 0,1; 1,0; 10...
The problem of personal identity in rape cases is solved by DNA analysis as a standard procedure forensic laboratories. However, fixing the exact time sexual abuse still an unsolved issue science. Here, we present our attempt to apply method Comet assay measure kinetics sperm degradation. Modification has enabled us obtain good correlation between ejaculation and test performance. We see this work proof principle that degradation could be used molecular clock for better estimation when...
Sulfadrugs, the first chemically-synthesised antibiotics, have saved millions of lives over past seven decades.Their effectiveness, however, is declining due to emergence drug resistance.Studies sulfadrug resistance in major pathogens, Pneumocystis and Plasmodium are impossible or difficult so our work has focused on development yeast models address action sulfa drugs, mechanisms develop new strategies for treatment.The sulfadrugs mimic p-aminobenzoate which condenses with dihydropteridine...